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Experimental Evolution of Insect Nuptial Food Gifts and Female Responses

昆虫结婚食品礼物和女性反应的实验进化

基本信息

批准号：
1654028
负责人：
Scott Sakaluk
金额：
$ 65万
依托单位：
Board of Trustees of Illinois State University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-05-01 至 2023-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1654028&HistoricalAwards=false
关键词：
Experimental Evolution Insect Nuptial Food

项目摘要

Insects have an enormous economic impact on society, negatively through their roles as agricultural pests and transmitters of disease, and positively as critical pollinators of a wide range of agricultural products. Their influence is amplified by their ability to reproduce rapidly and in great numbers, which is determined by the number of offspring each female can produce. Differences between females in their reproduction is partially controlled by males, who can manipulate females by transferring chemicals that influence their physiology and receptivity during mating. This project involves fundamental research to identify the chemicals transferred by males that influence female mating behavior, the underlying genes, and the mechanisms by which females protect themselves from these substances. These studies may lead to products that inhibit female reproduction, and which can be used for safe and effective biological control of insects. The project will also contribute to the training of the next generation of scientists by supporting undergraduate, graduate, and post-doctoral students. These students will disseminate their work through scientific scholarship, and will participate in community outreach programs centered on the public's fascination with insects. These include activities that foster an interest in science in elementary-school children and that enhance scientific literacy by providing resources for the study of insects to high school teachers. Nuptial food gifts are food items given by males to females and are an integral feature of various insect mating systems. In decorated crickets, nuptial food gifts take the form of a spermatophylax, a gelatinous mass forming part of the male's spermatophore and consumed by the female. Although nuptial gifts advance the fitness interests of males by maximizing sperm transfer, their effect on the fitness of females remains unclear. The researchers will employ experimental evolution to explore the role of conflicting male and female goals in driving the evolution of the chemical composition of male nuptial gifts and female responses to them in decorated crickets. Specifically, they will vary the intensity of selection and conflict by manipulating the ratio of males and females in replicate populations, and then monitor, simultaneously, the evolutionary responses of males and females. They will use gas chromatography and mass spectrometry to track evolutionary modifications in the free amino acid profile of the spermatophylax, and targeted gene expression to assess changes in key genes. Female evolutionary responses will be assessed using behavioral assays to assess the gustatory appeal of gifts, and a comparative transcriptomics approach will be used to characterize differential gene expression in brain and gut tissue of females from different selection lines. By coupling a powerful experimental evolution design with state-of-the art functional genomics, they aim to provide one of the most comprehensive examinations of how selection and conflict contribute to the evolution of a widespread reproductive adaptation in insects.

昆虫对社会产生了巨大的经济影响，它们作为农业害虫和疾病传播者的作用是消极的，而作为各种农产品的关键传粉者则是积极的。它们的影响力被它们快速和大量繁殖的能力所放大，这是由每只雌性能够产生的后代数量决定的。雌性之间的差异在一定程度上由雄性控制，雄性可以通过转移化学物质来操纵雌性，这些化学物质会在交配过程中影响雌性的生理和接受能力。该项目涉及基础研究，以确定雄性转移的影响雌性交配行为的化学物质、潜在的基因，以及雌性保护自己免受这些物质伤害的机制。这些研究可能导致抑制雌性生殖的产品，并可用于安全和有效的昆虫生物防治。该项目还将通过支持本科生、研究生和博士后学生，为培养下一代科学家做出贡献。这些学生将通过科学奖学金来传播他们的工作，并将参加以公众对昆虫的迷恋为中心的社区推广计划。这些活动包括培养小学生对科学的兴趣，以及通过向高中教师提供昆虫研究资源来提高科学素养。婚礼食物礼物是雄性送给雌性的食物，是各种昆虫交配系统的组成部分。在装饰的蟋蟀中，婚礼食物礼物以精原细胞的形式存在，这是一种凝胶状的物质，构成雄性精原细胞的一部分，由雌性消耗。尽管婚礼礼物通过最大限度地转移精子来促进男性的健康兴趣，但它们对女性健康的影响尚不清楚。研究人员将通过实验进化来探索男性和女性目标的冲突在推动男性结婚礼物的化学成分和女性对装饰蟋蟀的反应的进化中所起的作用。具体地说，它们将通过操纵复制种群中的雄性和雌性的比例来改变选择和冲突的强度，然后同时监测雄性和雌性的进化反应。他们将使用气相色谱和质谱学来跟踪精巢中游离氨基酸图谱的进化修改，并通过有针对性的基因表达来评估关键基因的变化。将通过行为分析来评估雌性进化反应，以评估礼物的味觉吸引力，并将使用比较转录转录方法来表征来自不同选择系的雌性大脑和肠道组织中的差异基因表达。通过将强大的实验性进化设计与最先进的功能基因组学相结合，他们的目标是提供最全面的研究之一，研究选择和冲突如何有助于昆虫广泛的生殖适应的进化。

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Male and female genotype and a genotype-by-genotype interaction mediate the effects of mating on cellular but not humoral immunity in female decorated crickets

DOI：
10.1038/s41437-020-00384-8
发表时间：
2020-11-20
期刊：
HEREDITY
影响因子：
3.8
作者：
Hampton, Kylie J.;Duffield, Kristin R.;Sadd, Ben M.
通讯作者：
Sadd, Ben M.

Effects of inbreeding on life-history traits and sexual competency in decorated crickets

近亲繁殖对装饰蟋蟀生活史特征和性能力的影响

DOI：
10.1016/j.anbehav.2019.05.027
发表时间：
2019
期刊：
Animal Behaviour
影响因子：
2.5
作者：
Sakaluk, Scott K.;Oldzej, Jeannine;Poppe, Christine J.;Harper, Jenny L.;Rines, Ian G.;Hampton, Kylie J.;Duffield, Kristin R.;Hunt, John;Sadd, Ben M.
通讯作者：
Sadd, Ben M.

Dynamic terminal investment in male burying beetles

雄性埋甲虫的动态终端投资

DOI：
10.1016/j.anbehav.2020.02.015
发表时间：
2020
期刊：
Animal Behaviour
影响因子：
2.5
作者：
Farchmin, Paige A.;Eggert, Anne-Katrin;Duffield, Kristin R.;Sakaluk, Scott K.
通讯作者：
Sakaluk, Scott K.

Transcriptomic responses of females to consumption of nuptial food gifts as a potential mediator of sexual conflict in decorated crickets