CAREER: Control Information Transfer in Complex DNA Structures

职业：控制复杂 DNA 结构中的信息传递

• 批准号: 1654485
• 负责人: Yonggang Ke
• 金额: $ 50万
• 依托单位: Emory University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1654485&HistoricalAwards=false
• 关键词: CAREER; Control; Information; Transfer; Complex

Non-technical: This award by the Biomaterials program in the Division of Materials Research to Emory University is to develop a novel DNA self-assembly paradigm that will allow scalable construction of large DNA structures that are complex and dynamic. Through evolution, biology showed the power of molecular self-assembly as it is shown in a huge variety of organisms in the planet that exhibit extremely sophisticated forms and functions via self-assembly of biomolecules, such as DNA and proteins. A key challenge in synthetic molecular self-assembly is to construct artificial, controllable systems that imitate intricate structures and complex behaviors seen in biological systems. This project is to harness the power of DNA self-assembly to design and construct scalable, modular, dynamic nanostructures that simulate some of the key aspects of information transfer observed in signaling cascades (e.g. T cell activation signaling cascades initiated by T cell receptor binding), including programmable initiation, propagation, and regulation of information transfer within the artificial DNA nanostructures. The project will provide an enabling platform for self-assembly of dynamic nanomaterials and nanodevices for a variety of important scientific research and applications. The students participating in this project will receive training in cutting-edge biomolecular assembly and nanoscience research. The research program will also be integrated with development of extensive educational outreach activities that are designed to recruit, educate and train the next generation scientists, and to increase the overall scientific literacy of the community, especially the underrepresented minority community in the Atlanta metropolitan area. Technical:Information transfer at the molecular level is an essential phenomenon in chemical and biological processes. This project aims to develop a novel molecular self-assembly paradigm to control long-range information transfer in artificial molecular arrays assembled from modular DNA structural units. The proposed studies on dynamic DNA molecular arrays are scalable, and their transformation can be initiated at selected units, then propagated to neighboring units through prescribed information pathways. Through systematic study of information transfer within 1D, 2D, and 3D dynamic DNA arrays, this project is expected to gain comprehensive understanding on: 1) the thermodynamic and kinetic behaviors of the DNA arrays; 2) the scalability and versatility of the new method; and 3) the programmable initiation, propagation, and regulation of information transfer in the DNA arrays. The new DNA arrays may be used as molecular devices to detect and translate molecular interactions to conformational changes in DNA structures, or to amplify single molecule signals (e.g. using FRET), via information propagation in the DNA arrays. The project would generate novel computational tools, physical models, and new courses that provide interdisciplinary training for postdocs, and graduate and undergraduate students. The outreach program will be tailored to educate and train the next generation scientists and to increase the overall scientific literacy of the community.

非技术性：该奖项由埃默里大学材料研究部的生物材料项目授予，旨在开发一种新的DNA自组装范例，该范例将允许可扩展地构建复杂和动态的大型DNA结构。通过进化，生物学显示了分子自组装的力量，因为它在地球上的各种生物体中显示，这些生物体通过DNA和蛋白质等生物分子的自组装表现出极其复杂的形式和功能。合成分子自组装的一个关键挑战是构建人工可控系统，模仿生物系统中的复杂结构和复杂行为。该项目是利用DNA自组装的力量来设计和构建可扩展的，模块化的，动态的纳米结构，模拟信号级联中观察到的信息传递的一些关键方面（例如由T细胞受体结合引发的T细胞活化信号级联），包括人工DNA纳米结构内信息传递的可编程启动，传播和调节。该项目将为各种重要的科学研究和应用提供一个动态纳米材料和纳米器件自组装的有利平台。参加该项目的学生将接受尖端生物分子组装和纳米科学研究的培训。该研究计划还将与旨在招募，教育和培训下一代科学家的广泛教育推广活动的发展相结合，并提高社区的整体科学素养，特别是亚特兰大大都市地区代表性不足的少数民族社区。技术：分子水平的信息传递是化学和生物过程中的一个基本现象。本项目旨在开发一种新的分子自组装模式，以控制由模块化DNA结构单元组装的人工分子阵列中的远程信息传递。所提出的动态DNA分子阵列的研究是可扩展的，它们的转换可以在选定的单元开始，然后通过规定的信息通路传播到相邻的单元。通过系统研究一维、二维和三维动态DNA阵列中的信息传递，本项目有望全面了解：1）DNA阵列的热力学和动力学行为; 2）新方法的可扩展性和通用性; 3）DNA阵列中信息传递的可编程启动、传播和调节。新的DNA阵列可用作分子装置，通过DNA阵列中的信息传播来检测分子相互作用并将其转化为DNA结构的构象变化，或放大单分子信号（例如使用FRET）。该项目将产生新的计算工具，物理模型和新的课程，为博士后，研究生和本科生提供跨学科的培训。该推广计划将专门用于教育和培训下一代科学家，并提高社区的整体科学素养。

项目成果

DNA‐Guided Assembly of Molecules, Materials, and Cells

• DOI: 10.1002/aisy.201900101
• 发表时间: 2019-12
• 期刊: Advanced Intelligent Systems
• 影响因子: 7.4
• 作者: Donglei Yang;Chunyan Zhou;Fei Gao;Pengfei Wang;Yonggang Ke

Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides

• DOI: 10.1021/jacs.7b08087
• 发表时间: 2017-10-11
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Jiang, Tao;Meyer, Travis A.;Ke, Yonggang
• 通讯作者: Ke, Yonggang

Programming the Curvatures in Reconfigurable DNA Domino Origami by Using Asymmetric Units

• DOI: 10.1021/acs.nanolett.0c03348
• 发表时间: 2020-11-11
• 期刊: NANO LETTERS
• 影响因子: 10.8
• 作者: Wang, Dongfang;Yu, Lei;Ke, Yonggang
• 通讯作者: Ke, Yonggang

Tunable DNA Origami Motors Translocate Ballistically Over μm Distances at nm/s Speeds

• DOI: 10.1002/anie.201916281
• 发表时间: 2020-04-01
• 期刊: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
• 影响因子: 16.6
• 作者: Bazrafshan, Alisina;Meyer, Travis A.;Salaita, Khalid
• 通讯作者: Salaita, Khalid

Dynamic DNA Structures

动态DNA结构

• DOI: 10.1002/smll.201900228
• 发表时间: 2019
• 期刊: Small
• 影响因子: 13.3
• 作者: Zhang Yingwei;Pan Victor;Li Xue;Yang Xueqin;Li Haofei;Wang Pengfei;Ke Yonggang
• 通讯作者: Ke Yonggang