Collaborative Research: Mapping interactions mediated by secondary metabolites in a fungus-growing ant microbiome

合作研究：绘制真菌生长的蚂蚁微生物组中次生代谢物介导的相互作用

• 批准号: 1656475
• 负责人: Jonathan Klassen
• 金额: $ 80.09万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656475&HistoricalAwards=false
• 关键词: Collaborative; Research; Mapping; interactions; mediated

Most microbes produce small molecule secondary metabolites as signals and/or antibiotics that control interactions between species. Despite their immense medical importance, the natural diversity and function of these molecules remains obscure. This prevents a full understanding of how microbial communities maintain critical ecosystem functions such as healthy host-microbe relationships. To further understand these systems, this project will generate networks of microbial interactions and their associated secondary metabolites based on the co-occurrence of particular microbes and secondary metabolites in environmental samples. Work will be conducted on the fungus-growing ant Trachymyrmex septentrionalis as an experimental model for these studies, profiling host-microbe interactions that are mediated by secondary metabolites within ant colonies collected throughout the Eastern USA. The T. septentrionalis symbiosis will also be utilized to broadly demonstrate the basics of animal-microbe interactions via a public display that will be linked to newly designed web resources. In addition, postdoctoral, graduate, and undergraduate researchers will be trained in microbiology and chemical ecology, including members of underrepresented groups. This project will therefore broadly advance understanding of the diversity and natural function of secondary metabolites and their impacts of host-microbe interactions.Nearly all microbes produce secondary metabolites. These molecules are particularly common in symbioses, where they mediate host-microbe interactions. Such interactions are typically studied using low-throughput approaches that remove secondary metabolite-producing organisms from their natural communities. These experiments are therefore insufficient to determine in situ secondary metabolite diversity, and cannot unambiguously link specific molecules to the ecological interactions that they naturally mediate. To overcome these limitations, this project will develop an approach to comprehensively identify interspecific interactions mediated by secondary metabolites by identifying patterns of co-variation between interacting taxa and the secondary metabolites that mediate these interactions, using the fungus-growing ant Trachymyrmex septentrionalis as an experimental model. In this approach, taxa that interact mutualistically will co-occur with both each other and the metabolite that mediates this mutualistic interaction. Reciprocally, taxa that interact antagonistically will rarely co-occur, and the metabolite that mediates this antagonistic interaction will co-occur with the producing taxon but not the target taxon. In situ interactions will be recapitulated using laboratory ant colonies microbial cultures. Researchers at various levels will be trained in these molecular and chemical ecology methods and museum and online resources to disseminate results to the broader public will be developed. Together, this project will provide a methodological approach to map interactions mediated by secondary metabolites in microbial communities and identify the in situ functions of such molecules.

大多数微生物产生小分子次生代谢物作为信号和/或控制物种间相互作用的抗生素。尽管它们在医学上具有巨大的重要性，但这些分子的自然多样性和功能仍然不清楚。这妨碍了对微生物群落如何维持关键生态系统功能的充分理解，例如健康的宿主-微生物关系。为了进一步了解这些系统，该项目将根据特定微生物和次生代谢物在环境样本中的共存情况，生成微生物相互作用及其相关次生代谢物的网络。将以真菌生长的蚂蚁Trachymyrmex Sepentrionaris作为这些研究的实验模型，描绘由在美国东部收集的蚂蚁群体内的次级代谢物介导的宿主与微生物的相互作用。北欧锥虫的共生也将被用来通过公共展示广泛地展示动物-微生物相互作用的基础，该公共展示将链接到新设计的网络资源。此外，博士后、研究生和本科生研究人员将接受微生物学和化学生态学方面的培训，包括代表人数不足的群体的成员。因此，该项目将广泛促进对次生代谢物的多样性和自然功能及其对宿主-微生物相互作用的影响的了解。几乎所有的微生物都会产生次生代谢物。这些分子在共生体中特别常见，在那里它们调节宿主和微生物的相互作用。这种相互作用通常使用低通量方法进行研究，这种方法将产生次生代谢物的生物体从其自然群落中移除。因此，这些实验不足以确定原位次生代谢物的多样性，也不能明确地将特定分子与它们自然调节的生态相互作用联系起来。为了克服这些限制，该项目将开发一种方法，通过识别相互作用的类群和介导这些相互作用的次生代谢物之间的协变模式，以生长真菌的蚂蚁Trachymyrmex Septentrionalis作为实验模型，全面识别由次生代谢物介导的种间相互作用。在这种方法中，相互作用的类群将彼此共生，并与调节这种互惠作用的代谢物共同出现。相反，相互拮抗的分类群将很少共生，调节这种拮抗相互作用的代谢物将与产生的分类单元共生，但不与目标分类单元共生。将使用实验室蚂蚁群体微生物培养重述原位相互作用。各级研究人员将接受这些分子和化学生态学方法的培训，并将开发博物馆和在线资源，向更广泛的公众传播结果。总之，该项目将提供一种方法学方法来绘制微生物群落中次级代谢物介导的相互作用图，并确定这些分子的原位功能。

项目成果

Bridging the Gap: Plant‐Endophyte Interactions as a Roadmap to Understanding Small‐Molecule Communication in Marine Microbiomes

弥合差距：植物与内生菌相互作用作为了解海洋微生物组中小分子通讯的路线图

• DOI: 10.1002/cbic.202000064
• 发表时间: 2020
• 期刊: ChemBioChem
• 影响因子: 3.2
• 作者: Samples, Robert M.;Balunas, Marcy J.
• 通讯作者: Balunas, Marcy J.

Evaluation of DESS as a storage medium for microbial community analysis

• DOI: 10.7717/peerj.6414
• 发表时间: 2019-02-05
• 期刊: PEERJ
• 影响因子: 2.7
• 作者: Lee, Kevin M.;Adams, Madison;Klassen, Jonathan L.
• 通讯作者: Klassen, Jonathan L.

Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing

• DOI: 10.1186/s12864-018-5381-7
• 发表时间: 2019-01-09
• 期刊: BMC GENOMICS
• 影响因子: 4.4
• 作者: Goldstein, Sarah;Beka, Lidia;Klassen, Jonathan L.
• 通讯作者: Klassen, Jonathan L.

Ecology helps bound causal explanations in microbiology

生态学有助于界定微生物学中的因果解释

• DOI: 10.1007/s10539-019-9728-5
• 发表时间: 2020
• 期刊: Biology & Philosophy
• 影响因子: 2.5
• 作者: Klassen, Jonathan L.

High-Quality Draft Genome Sequences of Eight Bacteria Isolated from Fungus Gardens Grown by Trachymyrmex septentrionalis Ants

• DOI: 10.1128/mra.00871-18
• 发表时间: 2018-07-01
• 期刊: MICROBIOLOGY RESOURCE ANNOUNCEMENTS
• 影响因子: 0.8
• 作者: Kopac, Sarah;Beatty, Hannah;Klassen, Jonathan L.
• 通讯作者: Klassen, Jonathan L.