Collaborative Research: Mapping interactions mediated by secondary metabolites in a fungus-growing ant microbiome

合作研究：绘制真菌生长的蚂蚁微生物组中次生代谢物介导的相互作用

• 批准号: 1656481
• 负责人: Pieter Dorrestein
• 金额: $ 31.22万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656481&HistoricalAwards=false
• 关键词: Collaborative; Research; Mapping; interactions; mediated

Most microbes produce small molecule secondary metabolites as signals and/or antibiotics that control interactions between species. Despite their immense medical importance, the natural diversity and function of these molecules remains obscure. This prevents a full understanding of how microbial communities maintain critical ecosystem functions such as healthy host-microbe relationships. To further understand these systems, this project will generate networks of microbial interactions and their associated secondary metabolites based on the co-occurrence of particular microbes and secondary metabolites in environmental samples. Work will be conducted on the fungus-growing ant Trachymyrmex septentrionalis as an experimental model for these studies, profiling host-microbe interactions that are mediated by secondary metabolites within ant colonies collected throughout the Eastern USA. The T. septentrionalis symbiosis will also be utilized to broadly demonstrate the basics of animal-microbe interactions via a public display that will be linked to newly designed web resources. In addition, postdoctoral, graduate, and undergraduate researchers will be trained in microbiology and chemical ecology, including members of underrepresented groups. This project will therefore broadly advance understanding of the diversity and natural function of secondary metabolites and their impacts of host-microbe interactions.Nearly all microbes produce secondary metabolites. These molecules are particularly common in symbioses, where they mediate host-microbe interactions. Such interactions are typically studied using low-throughput approaches that remove secondary metabolite-producing organisms from their natural communities. These experiments are therefore insufficient to determine in situ secondary metabolite diversity, and cannot unambiguously link specific molecules to the ecological interactions that they naturally mediate. To overcome these limitations, this project will develop an approach to comprehensively identify interspecific interactions mediated by secondary metabolites by identifying patterns of co-variation between interacting taxa and the secondary metabolites that mediate these interactions, using the fungus-growing ant Trachymyrmex septentrionalis as an experimental model. In this approach, taxa that interact mutualistically will co-occur with both each other and the metabolite that mediates this mutualistic interaction. Reciprocally, taxa that interact antagonistically will rarely co-occur, and the metabolite that mediates this antagonistic interaction will co-occur with the producing taxon but not the target taxon. In situ interactions will be recapitulated using laboratory ant colonies microbial cultures. Researchers at various levels will be trained in these molecular and chemical ecology methods and museum and online resources to disseminate results to the broader public will be developed. Together, this project will provide a methodological approach to map interactions mediated by secondary metabolites in microbial communities and identify the in situ functions of such molecules.

大多数微生物产生小分子次级代谢产物作为控制物种之间相互作用的信号和/或抗生素。尽管它们具有巨大的医学重要性，但这些分子的天然多样性和功能仍然不清楚。这阻碍了对微生物群落如何维持关键生态系统功能（如健康的宿主-微生物关系）的充分理解。为了进一步了解这些系统，该项目将根据环境样本中特定微生物和次级代谢物的共存来生成微生物相互作用及其相关次级代谢物的网络。工作将进行真菌生长的蚂蚁Trachymyrmex septentrionalis作为这些研究的实验模型，剖析宿主微生物的相互作用，介导的次级代谢产物收集在整个美国东部的蚂蚁殖民地。霸王还将利用septentrionalis symbiosis，通过与新设计的网络资源链接的公开展示，广泛展示动物-微生物相互作用的基本知识。此外，博士后，研究生和本科生研究人员将接受微生物学和化学生态学的培训，包括代表性不足的群体的成员。因此，该项目将广泛推进对次级代谢产物的多样性和天然功能及其对宿主-微生物相互作用的影响的理解。这些分子在共生体中特别常见，它们介导宿主-微生物相互作用。通常使用低通量方法研究这种相互作用，这些方法将产生次级代谢物的生物体从其自然群落中去除。因此，这些实验不足以确定原位次生代谢物的多样性，也不能明确地将特定分子与它们天然介导的生态相互作用联系起来。为了克服这些局限性，本项目将开发一种方法，以全面确定种间相互作用介导的次生代谢产物，通过确定相互作用的类群和介导这些相互作用的次生代谢产物之间的协变模式，使用真菌生长的蚂蚁Trachymyrmex septentrionalis作为实验模型。在这种方法中，相互作用的分类群将与彼此以及介导这种相互作用的代谢物共存。相反，拮抗相互作用的类群将很少共同出现，和代谢产物，介导这种拮抗相互作用将共同出现与生产类群，但不是目标taxon. In原位相互作用将重演使用实验室蚂蚁菌落微生物培养。各级研究人员将接受这些分子和化学生态学方法的培训，并将开发博物馆和在线资源，向更广泛的公众传播成果。总之，该项目将提供一种方法学途径来绘制微生物群落中次级代谢产物介导的相互作用，并确定这些分子的原位功能。

项目成果

Virulence as a Side Effect of Interspecies Interaction in Vibrio Coral Pathogens

• DOI: 10.1128/mbio.00201-20
• 发表时间: 2020-07
• 期刊: mBio
• 影响因子: 6.4
• 作者: Esther Rubio-Portillo;Ana-Belén Martín-Cuadrado;A. Caraballo-Rodríguez;F. Rohwer;P. Dorrestein;J. Antón

Untargeted mass spectrometry-based metabolomics approach unveils molecular changes in raw and processed foods and beverages

• DOI: 10.1016/j.foodchem.2019.125290
• 发表时间: 2020-01-01
• 期刊: FOOD CHEMISTRY
• 影响因子: 8.8
• 作者: Gauglitz, Julia M.;Aceves, Christine M.;Dorrestein, Pieter C.
• 通讯作者: Dorrestein, Pieter C.

MolNetEnhancer: Enhanced Molecular Networks by Integrating Metabolome Mining and Annotation Tools

• DOI: 10.3390/metabo9070144
• 发表时间: 2019-07-01
• 期刊: METABOLITES
• 影响因子: 4.1
• 作者: Ernst, Madeleine;Kang, Kyo Bin;van der Hooft, Justin J. J.
• 通讯作者: van der Hooft, Justin J. J.

Reproducible molecular networking of untargeted mass spectrometry data using GNPS

• DOI: 10.1038/s41596-020-0317-5
• 发表时间: 2020-05-13
• 期刊: NATURE PROTOCOLS
• 影响因子: 14.8
• 作者: Aron, Allegra T.;Gentry, Emily C.;Dorrestein, Pieter C.
• 通讯作者: Dorrestein, Pieter C.