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Iron uptake by marine bacteria: regulation and function of weak and strong siderophores

海洋细菌对铁的吸收：弱铁载体和强铁载体的调节和功能

基本信息

批准号：
1657639
负责人：
Francois Morel
金额：
$ 49.5万
依托单位：
Princeton University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-02-01 至 2020-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1657639&HistoricalAwards=false
关键词：
Iron uptake marine bacteria regulation

项目摘要

Organic molecules that bind and transport iron are called siderophores. Because iron is an essential trace element for biological systems and exists at very, very low concentrations in the open ocean, siderophores perform a critical role in capturing iron for cellular function. It is known that marine bacteria can produce two different types of siderophores that either tightly bind iron or only weakly do so, with different ecological consequences. This researcher will leverage an exceptional career on metal-organism interactions to examine the unsolved question of exactly what environmental and biochemical conditions (for example the availability of iron) control bacterial production of various siderophores. Results will generate significant new understanding of a critical chemical oceanographic process, and cap this researcher's groundbreaking discoveries that have built to this project. Funding for this research will also support the advancement of women in science by both providing the highest quality training of a female scientist and providing the opportunity for her to host an oceanography booth at the Princeton Plasma Physics Lab's "Young Women in Science" conference.This study will use Vibrio harveyi as a model organism to investigate a variety of questions surrounding the marine bacterial production of weak and strong siderophores. To start, the investigation will look into how siderophore production is controlled by varying iron availability and quorum sensing (i.e. a coordinated response correlated to population density and/or certain signaling molecules). This also includes in-depth investigation of the impact of life phase and biochemical changes with growth as they relate to coordinated use of weak and strong siderophores. Using established protocols for genetic manipulation of V. harveyi, the researcher plans to discover how varying combinations of weak and strong siderophores maximize the uptake of iron. The broader biogeochemical implications of this study to the field of chemical oceanography, with regard to the microbial use of, and cellular responses to, many essential micronutrients in the ocean would be to significantly influence understanding of elemental distributions beyond the specific study of iron and siderophore cycling in the ocean.

结合和运输铁的有机分子被称为铁载体。由于铁是生物系统必需的痕量元素，并且在开放的海洋中以非常低的浓度存在，铁载体在捕获铁以实现细胞功能方面发挥着关键作用。众所周知，海洋细菌可以产生两种不同类型的铁载体，它们要么紧密结合铁，要么只能微弱结合，产生不同的生态后果。这位研究人员将利用在金属-有机体相互作用方面的非凡职业生涯来研究尚未解决的问题，即到底是什么环境和生化条件(例如铁的可获得性)控制各种铁载体的细菌生产。这些结果将产生对一个关键的化学海洋学过程的重大新理解，并结束这位研究人员对该项目的突破性发现。这项研究的资金还将通过为女科学家提供最高质量的培训并为她提供机会在普林斯顿等离子体物理实验室的“科学中的年轻女性”会议上主持海洋学摊位来支持女性在科学领域的进步。这项研究将使用哈维氏弧菌作为模式生物来研究围绕着弱的和强的铁载体的海洋细菌产生的各种问题。首先，调查将研究铁的可获得性和群体感应(即与种群密度和/或某些信号分子相关的协调反应)如何控制铁载体的生产。这还包括深入调查生命阶段和生化变化对生长的影响，因为它们与弱铁载体和强铁载体的协调使用有关。利用已建立的对哈维氏弧菌进行遗传操作的方案，研究人员计划发现弱铁载体和强铁载体的不同组合如何最大限度地吸收铁。这项研究对化学海洋学领域的更广泛的生物地球化学影响，涉及海洋中许多基本微量营养素的微生物利用和细胞反应，将大大影响对元素分布的理解，而不仅仅是对海洋中铁和铁载体循环的具体研究。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Quorum sensing and iron regulate a two-for-one siderophore gene cluster in Vibrio harveyi

DOI：
10.1073/pnas.1805791115
发表时间：
2018-07-17
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
McRose, Darcy L.;Baars, Oliver;Morel, Francois M. M.
通讯作者：
Morel, Francois M. M.

Crochelins: Siderophores with an Unprecedented Iron-Chelating Moiety from the Nitrogen-Fixing Bacterium Azotobacter chroococcum