EAGER: Identification of molecular parameters defining the fine line between thermostability and thermophilic properties
EAGER:识别定义热稳定性和嗜热特性之间细微界限的分子参数
基本信息
- 批准号:1662080
- 负责人:
- 金额:$ 19.31万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-02-01 至 2020-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Enzymes from thermophilic organisms are often more suitable for applications in biotechnology because they are thermostable and have optimal activities at high temperatures. This project will use novel experimental and computational approaches to define the molecular basis for these highly useful properties. Ultimately, these studies will provide important information for the selection of enzymes that are stable and optimally active at high temperatures and for the engineering of such enzymes for applications in biotechnology. This project will contribute to the training of undergraduate and graduate students and will be incorporated into outreach efforts to the public. The elucidation of molecular principles that render proteins more stable under extreme conditions such as high temperatures has been subject of a large body of research. Earlier work suggested that thermophilic variants of enzymes have more polar interactions or more H-bonds or increased hydrophobic interactions, better packing of hydrophobic core or some combination of them. Other studies showed that some of these properties were not applicable to many other mesophilic to thermophilic comparisons and more subtle and distributed effects and dynamic properties of proteins may play a significant role in attaining thermophilicity. Sequence-based comparisons also failed to explain the basis of thermophilicity and so far no clear and unifying explanation has emerged. This project aims to determine dynamic and thermodynamic properties that separate thermophilic enzymes from their mesophilic counterparts by dissecting the effects of protein dynamics and solvent reorganization on the enzyme ligand interactions. To do this, thermophilic and mesophilic variants of the same protein (aminoglycoside-N3-nucleotidyltransferase) will be used; these variants have minimal differences in the primary sequence.
来自嗜热生物的酶通常更适合于生物技术中的应用,因为它们是热稳定的,并且在高温下具有最佳活性。 该项目将使用新的实验和计算方法来定义这些非常有用的特性的分子基础。 最终,这些研究将为选择在高温下稳定和最佳活性的酶以及为生物技术中应用的此类酶的工程设计提供重要信息。 该项目将有助于培训本科生和研究生,并将纳入对公众的外联工作。阐明使蛋白质在极端条件如高温下更稳定的分子原理一直是大量研究的主题。 早期的工作表明,酶的嗜热变体具有更多的极性相互作用或更多的H-键或增加的疏水相互作用,更好地包装疏水核心或它们的某种组合。 其他研究表明,这些性质中的一些不适用于许多其他嗜温性与嗜热性的比较,并且蛋白质的更微妙和分布的效应和动力学性质可能在获得嗜热性方面发挥重要作用。 基于序列的比较也未能解释嗜热性的基础,到目前为止还没有出现明确和统一的解释。 该项目旨在通过解剖蛋白质动力学和溶剂重组对酶配体相互作用的影响来确定将嗜热酶与其嗜温酶分离的动力学和热力学性质。 为此,将使用相同蛋白质(氨基糖苷-N3-核苷酸转移酶)的嗜热和嗜温变体;这些变体在一级序列中具有最小差异。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The Thermodynamics of Ligand Binding to the Aminoglycoside O- Nucleotidyltransferase(4′) and Variants Yields Clues about Thermophilic Properties
配体与氨基糖苷 O-核苷酸转移酶 (4-2) 和变体结合的热力学提供了有关嗜热特性的线索
- DOI:10.1021/acs.biochem.8b01201
- 发表时间:2019
- 期刊:
- 影响因子:2.9
- 作者:Kocaman, Seda;Serpersu, Engin H.
- 通讯作者:Serpersu, Engin H.
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Gladys Alexandre其他文献
Digital holographic microscopy for bacterial species classification and motility characterization
用于细菌种类分类和运动表征的数字全息显微镜
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
Jian Gao;Hadi Parsian;Jarl Carnahan;Gladys Alexandre;James Trolinger - 通讯作者:
James Trolinger
Gladys Alexandre的其他文献
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{{ truncateString('Gladys Alexandre', 18)}}的其他基金
Mechanisms linking bacterial chemotaxis signaling to nitrogen fixation in beneficial plant-associated bacteria
将细菌趋化信号与有益植物相关细菌固氮联系起来的机制
- 批准号:
2130556 - 财政年份:2021
- 资助金额:
$ 19.31万 - 项目类别:
Continuing Grant
Chemotaxis sensing preference in plant-microbe associations
植物-微生物关联中的趋化性传感偏好
- 批准号:
1715185 - 财政年份:2017
- 资助金额:
$ 19.31万 - 项目类别:
Standard Grant
Real Time Chemotaxis in Commensal Plant-microbe Associations
共生植物-微生物关联中的实时趋化性
- 批准号:
1330344 - 财政年份:2013
- 资助金额:
$ 19.31万 - 项目类别:
Continuing Grant
Chemotaxis And The Regulation Of Multiple Cellular Functions In A Bacterium
细菌的趋化性和多种细胞功能的调节
- 批准号:
0919819 - 财政年份:2009
- 资助金额:
$ 19.31万 - 项目类别:
Standard Grant
CAREER: Chemosensory Behavior in Plant-Microbe Association
职业:植物-微生物关联中的化学感应行为
- 批准号:
0622277 - 财政年份:2005
- 资助金额:
$ 19.31万 - 项目类别:
Continuing Grant
CAREER: Chemosensory Behavior in Plant-Microbe Association
职业:植物-微生物关联中的化学感应行为
- 批准号:
0347218 - 财政年份:2004
- 资助金额:
$ 19.31万 - 项目类别:
Continuing Grant
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