Biogeochemistry of Iron, Iodine and Boron in Marine Plant-like Linages: Synchrotron Radiation Methods for Visualizing Chemical State, Localization and Potential Interactions

海洋植物类谱系中铁、碘和硼的生物地球化学：用于可视化化学状态、定位和潜在相互作用的同步辐射方法

• 批准号: 1664657
• 负责人: Carl Carrano
• 金额: $ 14.51万
• 依托单位: San Diego State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2022-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1664657&HistoricalAwards=false
• 关键词: Biogeochemistry; Iron; Iodine; Boron; Marine

With this award the Chemistry of Life Processes Program of the Chemistry Division of NSF are supporting Carl J. Carrano and Frithjof C. Kupper's study of how important trace elements (iron, boron and iodine) are taken up and stored in seaweeds, and how these elements interact with each other so as to affect the plant biology. Seaweeds represent an important resource with a wide range of uses in the food, cosmetic, and fertilizer industries. They are also attracting increasing attention as a source for biofuels that do not compete with terrestrial plants for food production. The project utilizes advanced high resolution synchrotron radiation methods to define the precise locations and chemical states of iron, iodine and boron in two important seaweeds and uses this information to assist in determining the biological importance of interactions between them. This work also provide training for graduate and undergraduate students in a wide range of important techniques. In addition, since San Diego State University has a high minority population (10th largest grantor of minority bachelor's degrees in the USA), this research project is thus expected to favorably impact minority training in the sciences. Outreach activities are also in progress.While in the cytoplasm many metalloproteins are present as a complicated mixture not easily amenable to simple analysis without prior potentially destructive, and certainly disruptive, isolation and purification. Many other metalloproteins are stored, sequestered or localized in discrete locations within the cell/organelles and are also difficult to isolate and study. As the resolution and flux density of synchrotron radiation (SR) increases, it may be possible to pinpoint such systems and analyze and characterize them directly in situ without need for isolation or purification. Thus, one goal of this research is to see what the limits are for SR methods for visualizing the chemical state, localization and potential interactions of trace elements as applied to biological samples given the increasing capabilities of 3rd generation synchrotrons where significant progress (both in terms of brilliance and resolution) has been evident. The project is using utilizing advanced high resolution (micron to submicron) SR methods, i.e. microprobe, 3D tomography, IR/Raman, XANES and EXAFS to define the precise locations and chemical states of iron, iodine and boron in two model marine macroalgae (Ectocarpus siliculosis, Macrocystis pyrifera). The investigators use this information to assist in determining the biological importance of interactions between the elements. Seaweeds represent an important resource with a wide range of uses in the food, cosmetic, and fertilizer industries. They are also attracting increasing attention as a source for biofuels that do not compete with terrestrial plants for food production.

有了这个奖项，NSF化学部的生命过程化学计划将支持卡尔·J·卡拉诺和弗里思约夫·C·Kupper研究了海藻如何吸收和储存重要的微量元素（铁、硼和碘），以及这些元素如何相互作用从而影响植物生物学。海藻是一种重要的资源，在食品、化妆品和肥料工业中具有广泛的用途。它们作为不与陆地植物竞争粮食生产的生物燃料来源也越来越受到关注。该项目利用先进的高分辨率同步辐射方法来确定铁、碘和硼在两种重要海藻中的精确位置和化学状态，并利用这一信息来协助确定它们之间相互作用的生物重要性。这项工作还为研究生和本科生提供了广泛的重要技术培训。 此外，由于圣地亚哥州立大学少数民族人口众多（美国第十大少数民族学士学位授予者），因此预计该研究项目将对少数民族的科学培训产生有利影响。在细胞质中，许多金属蛋白是一种复杂的混合物，如果不事先进行可能具有破坏性的分离和纯化，就不容易进行简单的分析。 许多其他金属蛋白被储存，隔离或定位在细胞/细胞器内的离散位置，也难以分离和研究。随着同步辐射（SR）的分辨率和通量密度的增加，有可能精确定位这样的系统，并直接在原位分析和表征它们，而不需要分离或纯化。因此，本研究的一个目标是看到SR方法的限制是什么，用于可视化的化学状态，局部化和潜在的相互作用的微量元素应用于生物样品，鉴于第三代同步加速器的能力不断提高，其中显着的进步（无论是在亮度和分辨率方面）是显而易见的。该项目利用先进的高分辨率（微米至亚微米）SR方法，即微探针、三维层析、IR/拉曼、XANES和EXAFS，确定两种模式海洋大型藻类（Ectocarpus siliculosis和Macrocystis pyrifera）中铁、碘和硼的精确位置和化学状态。 研究人员使用这些信息来帮助确定元素之间相互作用的生物重要性。海藻是一种重要的资源，在食品、化妆品和肥料工业中具有广泛的用途。它们作为不与陆地植物竞争粮食生产的生物燃料来源也越来越受到关注。

项目成果

Iron and Harmful Algae Blooms: Potential Algal-Bacterial Mutualism Between Lingulodinium polyedrum and Marinobacter algicola

铁和有害藻类大量繁殖：Lingulodinium polyedrum 和 Marinobacter algicola 之间潜在的藻类-细菌互惠关系

• DOI: 10.3389/fmars.2018.00180
• 发表时间: 2018
• 期刊: Frontiers in Marine Science
• 影响因子: 3.7
• 作者: Yarimizu, Kyoko;Cruz-López, Ricardo;Carrano, Carl J.
• 通讯作者: Carrano, Carl J.

The influence of marine algae on iodine speciation in the coastal ocean

海藻对近海碘形态的影响

• DOI: 10.4490/algae.2020.35.5.25
• 发表时间: 2020
• 期刊: ALGAE
• 影响因子: 3.2
• 作者: Carrano, Mary W.;Yarimizu, Kyoko;Gonzales, Jennifer L.;Cruz-López, Ricardo;Edwards, Matthew S.;Tymon, Teresa M.;Küpper, Frithjof C.;Carrano, Carl J.
• 通讯作者: Carrano, Carl J.

Distribution of dissolved iron and bacteria producing the photoactive siderophore, vibrioferrin, in waters off Southern California and Northern Baja

南加州和北巴哈海域溶解的铁和产生光活性铁载体、弧菌铁蛋白的细菌的分布

• DOI: 10.1007/s10534-018-00163-3
• 发表时间: 2019
• 期刊: BioMetals
• 影响因子: 3.5
• 作者: Yarimizu, Kyoko;Cruz-López, Ricardo;García-Mendoza, Ernesto;Edwards, Matthew;Carter, Melissa L.;Carrano, Carl J.
• 通讯作者: Carrano, Carl J.