RAPID: Waterborne Elizabethkingia disinfection studies in response to ongoing U.S. outbreak

RAPID：水性 Elizabethkingia 消毒研究，以应对美国持续爆发的疫情

• 批准号: 1700604
• 负责人: Brooke Mayer
• 金额: $ 4.99万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-01-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1700604&HistoricalAwards=false
• 关键词: RAPID; Waterborne; Elizabethkingia; disinfection; studies

1700604MayerThere is an ongoing outbreak in the U.S caused by Elizabethkingia bacteria. Elizabethkingia is a ubiquitous group of bacteria whose presence in soil and water ordinarily pose little risk to human health. However, the current outbreak includes 63 confirmed cases, and has been associated with 18 confirmed fatalities since November 2015. This opportunistic human pathogen is particularly alarming because it tends to exhibit a high degree of antibiotic resistance and mortality. Given the emergence of this pathogen and the ongoing outbreak, it is important to develop a better understanding of how to effectively control these bacteria in drinking water. This RAPID research project will provide the first direct assessment of inactivation of waterborne Elizabethkingia bacteria using chlorination, chloramination, ozonation, and ultraviolet (UV) irradiation. Specific objectives of the proposed work include the following. Characterize the disinfection response of: 1) planktonic cells, 2) attached cells, and 3) develop a deeper understanding of the relationship between disinfection efficacy and Elizabethkingia composition. Laboratory experiments will be used to establish concentration (or UV intensity) x time relationships (referred to as CT relationships). This disinfectant response data is currently unknown, and is urgently needed to develop effective disinfection strategies to curtail the current outbreak and avoid future public health disasters. Additionally, this project will probe the relationship between the bacteria's composition (nucleic and amino acids) and susceptibility to different modes of disinfection. The proposed research will substantially contribute to advancing broader societal outcomes including improved understanding of drinking water disinfection strategies to better protect human health, which has immediate implications for curtailing the ongoing outbreak caused by Elizabethkingia bacteria and for preventing future outbreaks. Findings from this project will also be integrated into course modules/discussions in the PIs regular teaching rotation, a summer experience for high school students, and at the Emerging Contaminants Short Course offered annually at Marquette University for academics and professionals.

1700604MayerThere is a going outbreak in the US caused by Escherichkingia bacteria.嗜酸菌是一种普遍存在的细菌，它们在土壤和水中的存在通常对人类健康构成的风险很小。然而，目前的疫情包括63例确诊病例，自2015年11月以来已与18例确诊死亡病例有关。这种机会性人类病原体特别令人担忧，因为它往往表现出高度的抗生素耐药性和死亡率。鉴于这种病原体的出现和持续的爆发，更好地了解如何有效控制饮用水中的这些细菌是很重要的。这个快速研究项目将提供第一个直接评估的灭活水环境中使用氯化，氯胺化，臭氧化，紫外线（UV）照射。拟议工作的具体目标如下。表征以下各项的消毒反应：1）寄生细胞，2）附着细胞，以及3）更深入地了解消毒功效与嗜酸菌成分之间的关系。实验室实验将用于建立浓度（或UV强度）x时间关系（称为CT关系）。这种消毒剂反应数据目前尚不清楚，迫切需要制定有效的消毒策略，以遏制目前的疫情，避免未来的公共卫生灾难。此外，该项目将探讨细菌的组成（核酸和氨基酸）与对不同消毒模式的敏感性之间的关系。拟议的研究将大大有助于推动更广泛的社会成果，包括提高对饮用水消毒策略的理解，以更好地保护人类健康，这对减少由嗜酸菌引起的持续爆发和预防未来爆发具有直接影响。该项目的研究结果也将被纳入课程模块/讨论中，包括PI定期教学轮换、高中生暑期体验以及马奎特大学每年为学者和专业人士提供的新兴污染物短期课程。