DISSERTATION RESEARCH: The role of phyllosphere microbiomes in plant species coexistence and plant-pathogen interactions

论文研究：叶际微生物组在植物物种共存和植物与病原体相互作用中的作用

• 批准号: 1701816
• 负责人: Keith Clay
• 金额: $ 2.01万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1701816&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; role; phyllosphere; microbiomes

Microorganisms are species that are so small that you cannot see them with the naked eye. Microorganisms live all over the surfaces and inside of every type of larger organism on the planet, forming what is known as the "microbiome" of the larger hosts. This research will focus on the microbiomes of plants, which have profound impacts on plant health and performance. This research will focus on how microbiomes affect resistance of a host to another microorganism: a disease-causing fungus. By experimentally manipulating combinations of microbiomes, host species, and whether or not the disease-causing fungus is present, the researchers will be able to find out how microbiomes affect the ability of different plant species to compete with one another and to resist disease. By growing microbes found in natural microbiomes and by using state-of-the-art DNA sequencing approaches, they will begin to identify how important microbial players affect plant health. This project will involve the training of a graduate student in cutting-edge bioinformatics techniques. Because one outcome of this work will be to identify how and whether inoculating plants with the right microbiome protects against disease, the results from this work will also contribute to the development of new approaches to fighting costly pathogens in crops, and to helping land managers promote biodiversity in natural systems. In the past three decades, the plant-soil feedback framework has been used to achieve theoretical and empirical breakthroughs. Phyllosphere microbial communities, which reside in or on aboveground plant tissues, are similarly diverse, but current research suffers from an inability to connect presence and identity of phyllosphere microbes to larger ecological questions. In this project, researchers will test how pathogen infection alters the fitness and growth outcomes of con- vs. heterospecific phyllosphere microbial communities, with a focus on the fungal constituents of these microbiomes. Next-generation sequencing technologies will be combined with culture-dependent approaches to study the mechanisms of fungal community assembly in the phyllosphere. Functional trait data will be collected on select fungal cultures as metrics of plant-fungal and pathogen-fungal interactions. This trait-based approach will then be used to determine whether specific traits of the fungi measured in the lab can be used to understand their abundance and role on plants. This body of work has the potential to expand the scientific community's understanding of phyllosphere community assembly, and the mechanisms by which different phyllosphere communities inhibit or facilitate plant-species coexistence on their own or in conjunction with pathogens.

微生物是非常小的物种，你不能用肉眼看到它们。 微生物生活在地球上每一种较大生物的表面和内部，形成了所谓的较大宿主的“微生物组”。这项研究将集中在植物的微生物组，这对植物的健康和性能有深远的影响。 这项研究将集中在微生物组如何影响宿主对另一种微生物的抵抗力：致病真菌。通过实验操纵微生物组、宿主物种以及致病真菌是否存在的组合，研究人员将能够发现微生物组如何影响不同植物物种相互竞争和抵抗疾病的能力。通过培养在天然微生物组中发现的微生物，并使用最先进的DNA测序方法，他们将开始确定微生物对植物健康的重要影响。该项目将涉及对一名研究生进行尖端生物信息学技术方面的培训。由于这项工作的成果之一将是确定具有正确微生物组的植物如何以及是否可以抵御疾病，这项工作的结果也将有助于开发新方法来对抗作物中昂贵的病原体，并帮助土地管理者促进自然系统中的生物多样性。在过去的三十年里，植物-土壤反馈框架已经被用来实现理论和经验的突破。叶圈微生物群落，居住在地上植物组织中或地上，同样是多样化的，但目前的研究无法连接的存在和叶圈微生物的身份更大的生态问题。在这个项目中，研究人员将测试病原体感染如何改变con-vs.异特异性叶圈微生物群落的适应性和生长结果，重点是这些微生物群的真菌成分。下一代测序技术将与依赖培养的方法相结合，研究叶际真菌群落组装的机制。功能性状数据将收集在选择的真菌培养物作为植物-真菌和病原体-真菌相互作用的指标。然后，这种基于特征的方法将用于确定实验室中测量的真菌的特定特征是否可用于了解它们在植物上的丰度和作用。这项工作有可能扩大科学界对叶圈群落组装的理解，以及不同叶圈群落抑制或促进植物物种共存的机制。