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Development of dielectrophoresis chromatography employing asymmetric insulating structures and electric fields

采用不对称绝缘结构和电场的介电泳色谱的开发

基本信息

批准号：
1705895
负责人：
Blanca Lapizco-Encinas
金额：
$ 29.96万
依托单位：
Rochester Institute of Tech
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1705895&HistoricalAwards=false
关键词：
Development dielectrophoresis chromatography employing asymmetric

项目摘要

A lab-on-a-chip is capable of automatically performing multiple complex chemical diagnostics in an electronic device that will easily fit on your thumb. As a lab-on-a chip manipulates an extremely small volume of fluid, it is also termed a microfluidic device. Miniaturization enables portability and accessibility, and opens the door for taking the chemical laboratory to where it is needed, be that a production line, a remote medical clinic, a sensitive environment, or real time monitoring of food and water safety. From a technical standpoint, miniaturization leads to higher resolution and sensitivity, improved integration, lower cost, and an extremely rapid processing time. To manipulate and sort biological particles, such as cells and complex macromolecules, dielectrophoresis is one of the most common techniques, as it relies on differences in particle response to an applied electric field. Living and dead cells respond differently to a nonuniform electric field than dead cells, as do cells from different organisms. Insulator-based dielectrophoresis adds another "knob" for particle sorting, with the placement of three-dimensional insulating structures as obstacles in the path of particle migration which "squeeze" the electric field. Theory predicts that using asymmetric obstacles provides yet another "knob" to sort particles. This project will experimentally validate the use of asymmetric insulating structures and nonuniform electric fields to sort and isolate biological particles in a microfluidic device. Asymmetric insulating dielectrophoresis will induce acceleration in some biological particles, and deceleration in others, with instantaneous velocity determined by particle geometry and charge. Differences in acceleration will lead to different residence times, creating a separation that is similar to a chromatographic process, creating the first demonstration of dielectrophoretic chromatography. A combination of carefully designed insulating post geometries with customized DC-biased, low frequency AC electric signals in insulator-based dielectrophoresis will be used to screen various bioparticles of interest, with variations in size, shape, and charge. The chromatographic effect will lead to distinct interaction with the stationary phase, and differential migration of the particles through the device. Particles will be eluted from the stationary phase as a ?peak? of concentrated particles and directed to a specific reservoir within the device for collection. This project will advance the state of the art in microfluidics by developing a new technique to sort and isolate particles. The present project will provide a premier research opportunity for undergraduate and graduate students, and will also assist in providing research opportunities for female students through the Women in Engineering programs.

芯片上的实验室能够在一个电子设备中自动执行多种复杂的化学诊断，这将很容易适合你的拇指。由于芯片上的实验室操作极小体积的流体，它也被称为微流体装置。小型化实现了便携性和可访问性，并为将化学实验室带到需要的地方打开了大门，无论是生产线，远程医疗诊所，敏感环境还是对食品和水安全的实时监控。从技术角度来看，小型化导致更高的分辨率和灵敏度，改进的集成度，更低的成本和极快的处理时间。为了操纵和分类生物粒子，如细胞和复杂的大分子，电泳术是最常用的技术之一，因为它依赖于粒子对外加电场的反应差异。与死细胞相比，活细胞和死细胞对非均匀电场的反应不同，来自不同生物体的细胞也是如此。基于绝缘体的介质电泳为粒子分选增加了另一个“旋钮”，在粒子迁移路径上放置三维绝缘结构作为障碍物，“挤压”电场。理论预测，使用不对称障碍物提供了另一个“旋钮”来对粒子进行分类。本项目将实验验证使用不对称绝缘结构和非均匀电场在微流控装置中对生物颗粒进行分类和分离。不对称绝缘介质电泳会导致一些生物粒子加速，而另一些生物粒子减速，其瞬时速度由粒子的几何形状和电荷决定。加速度的差异将导致不同的停留时间，创建类似于色谱过程的分离，创建介电色谱的第一个演示。精心设计的绝缘柱几何形状与定制的直流偏置、低频交流电信号在绝缘子基介电电泳中的组合将用于筛选各种感兴趣的生物颗粒，具有大小、形状和电荷的变化。色谱效应将导致与固定相的不同相互作用，以及颗粒通过该装置的不同迁移。粒子将从固定相中洗脱出来，形成一个峰。浓缩的颗粒，并被引导到设备内的特定储存器中进行收集。该项目将通过开发一种分类和分离颗粒的新技术来推进微流体技术的发展。本项目将为本科生和研究生提供一个重要的研究机会，并将通过“工程中的女性”项目协助为女学生提供研究机会。