EAGER: Linking the Chlorine and Carbon Cycles in the Arctic Coastal Plain

EAGER：将北极沿海平原的氯和碳循环联系起来

• 批准号: 1712774
• 负责人: David Lipson
• 金额: $ 21.14万
• 依托单位: San Diego State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1712774&HistoricalAwards=false
• 关键词: EAGER; Linking; Chlorine; Carbon; Cycles

The element, chlorine, is not normally studied in natural environments, except in areas that have been contaminated with toxic compounds like pesticides, industrial solvents or radioactive 36Cl. It is often assumed that chlorine enters non-contaminated ecosystems mostly in the form of chloride (the same negative ion in table salt), and that chloride does not interact with plants or soil microorganisms. However, there is growing evidence that chloride is taken up and transformed by plants and soil microorganisms into complex chlorine-containing organic compounds. In environments where oxygen is scarce, some bacteria can use these chlorinated organic compounds instead of oxygen in a form of anaerobic respiration called organohalide respiration (OHR). In this way, these bacteria can quickly use up energy sources that would otherwise be used to produce methane. This means that an active biological chlorine cycle could reduce the amount of methane that is released into the atmosphere. Methane is a strong greenhouse gas, trapping about 30 times as much heat per molecule as carbon dioxide. The Arctic region has been warming faster than the rest of the planet, and large amounts of organic carbon are stored in Arctic soils. It is thus important to understand how much soil carbon will be lost to the atmosphere in the form of carbon dioxide or methane, since these two gases have different effects on the climate the climate system. This project measures rates of biological chlorine cycling in locations across the Arctic Coastal Plain of northern Alaska, and tests whether organohalide respiration does in fact significantly reduces methane production in these areas. This project could inform models of greenhouse gas emissions, improve understanding of the fate of chlorinated contaminants in Arctic soils, and further the basic science of biological chlorine cycling. The project will involve students at a minority-serving institution (San Diego State University) and a high school teacher, who will lead broader outreach and education efforts.The proposed research addresses the following two questions: (1) Does OHR inhibit methanogenesis via competition for H2? (2) How does the relative magnitude of Cl cycling and its relationship to CH4 flux change along a coastal-inland gradient in the Arctic Coastal Plain? The experimental approach consists of a field survey that compares CH4 fluxes and indicators of Cl cycling along a gradient of coastal influence from Barrow to the foothills of the Brooks Range, and a laboratory incubation experiment to study the relationships among OHR, methanogenesis, other terminal electron acceptor processes, and H2 availability. Indicators of Cl cycling include sizes and transformation rates of soil Cl pools, metagenomes describing the relative abundance of genes and microbial taxa associated with Cl cycling and other anaerobic processes, and 37Cl and 36Cl isotopic analysis to infer the dominant Cl cycling processes and to constrain long-term cycling rates. The laboratory incubation will follow anaerobic processes (OHR, iron reduction, methanogenesis, acetogenesis, and sulfate reduction) in microcosms varying in Clorg and H2 concentration to establish the thermodynamic hierarchy among these processes and whether competition is alleviated by increased H2.

氯元素通常不会在自然环境中进行研究，除非是在被农药，工业溶剂或放射性36 Cl等有毒化合物污染的地区。人们通常认为氯主要以氯化物（食盐中的负离子）的形式进入未受污染的生态系统，并且氯化物不会与植物或土壤微生物相互作用。然而，越来越多的证据表明，氯被植物和土壤微生物吸收并转化为复杂的含氯有机化合物。在氧气稀缺的环境中，一些细菌可以使用这些氯化有机化合物代替氧气，以一种称为有机卤化物呼吸（OHR）的厌氧呼吸形式存在。通过这种方式，这些细菌可以快速耗尽原本用于生产甲烷的能源。这意味着活跃的生物氯循环可以减少释放到大气中的甲烷量。甲烷是一种强烈的温室气体，每个分子捕获的热量是二氧化碳的30倍。北极地区的变暖速度比地球上其他地区更快，大量的有机碳储存在北极土壤中。因此，重要的是要了解有多少土壤碳将以二氧化碳或甲烷的形式损失到大气中，因为这两种气体对气候系统有不同的影响。该项目测量了阿拉斯加北方北极沿海平原的生物氯循环速率，并测试了有机卤化物呼吸是否确实显著减少了这些地区的甲烷产量。该项目可以为温室气体排放模型提供信息，提高对北极土壤中氯化污染物命运的理解，并促进生物氯循环的基础科学。该项目将涉及学生在少数民族服务机构（圣地亚哥州立大学）和高中教师，谁将领导更广泛的推广和教育effortes.The拟议的研究解决了以下两个问题：（1）OHR抑制甲烷通过竞争H2？(2)在北极沿海平原，Cl循环的相对大小及其与CH 4通量的关系如何沿着沿海-内陆梯度变化？实验方法包括一个实地调查，比较CH 4通量和Cl循环沿着梯度的沿海影响的巴罗的布鲁克斯范围的山麓指标，和一个实验室培养实验，研究OHR，甲烷生成，其他终端电子受体过程之间的关系，和H2可用性。Cl循环的指标包括土壤Cl库的大小和转化率，描述与Cl循环和其他厌氧过程相关的基因和微生物类群的相对丰度的宏基因组，以及37 Cl和36 Cl同位素分析，以推断主导Cl循环过程并限制长期循环速率。实验室孵育将遵循Clorg和H2浓度变化的微观世界中的厌氧过程（OHR、铁还原、甲烷生成、乙酸生成和硫酸盐还原），以建立这些过程之间的热力学等级以及竞争是否通过增加H2而减轻。

项目成果

Biological chlorine cycling in the Arctic Coastal Plain

• DOI: 10.1007/s10533-017-0359-0
• 发表时间: 2017-07
• 期刊: Biogeochemistry
• 影响因子: 4
• 作者: Jaime E. Zlamal;T. Raab;M. Little;Robert Edwards;D. Lipson

Organohalide-Respiring Bacteria at the Heart of Anaerobic Metabolism in Arctic Wet Tundra Soils

• DOI: 10.1128/aem.01643-20
• 发表时间: 2020-11
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: D. Lipson;T. Raab;Sherlynette Pérez Castro;Alexander Powell

Snow melt stimulates ecosystem respiration in Arctic ecosystems

• DOI: 10.1111/gcb.15193
• 发表时间: 2020-06-30
• 期刊: GLOBAL CHANGE BIOLOGY
• 影响因子: 11.6
• 作者: Arndt, Kyle A.;Lipson, David A.;Zona, Donatella
• 通讯作者: Zona, Donatella