EAPSI: Developing Single Nucleus ATAC-seq to Map the Ageing Epigenome

EAPSI：开发单核 ATAC-seq 来绘制衰老表观基因组图谱

• 批准号: 1714070
• 负责人: Siti-Nur-Sarah Morris
• 金额: $ 0.54万
• 依托单位: Morris Siti-Nur-Sarah
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1714070&HistoricalAwards=false
• 关键词: EAPSI; Developing; Single; Nucleus; ATAC

Insights into the molecular basis of aging are key to developing diagnostic and therapeutic methods for alleviating age-related disorders. However, one of the difficulties in creating a general age panacea is the fact that each cell within an organism ages differently. Therefore, one of the goals for aging research is to characterize how individual cells change over time. This project will focus on muscle cells because muscle weakness is a common complaint in the elderly. Single cell studies are usually difficult due to the small amount of source material. Advances in sequencing and cell collection technology provide an opportunity for research into single cell genomes. This research project utilizes facilities at RIKEN Yokohama to access the latest in single cell technologies and to benefit from the leadership of Dr. Aki Minoda.The purpose of this project is to develop a single nucleus Assay for Transposase-Accessible Chromatin sequencing (snATAC-seq) protocol for the characterization of epigenetic heterogeneity in mouse skeletal muscle. While single cell ATAC-seq is available, viable snATAC-seq remains undeveloped. ATAC-seq is a novel method to determine the status of epigenetic chromatin modifications. Unlike other forms of sequencing, ATAC-seq is able to map nucleosome positions, thereby providing a multidimensional description of the cellular epigenome. Application of this promising technology to the question of ageing and ageing-related disease opens up new avenues of discourse and possible therapeutics. Development of single nucleus ATAC-seq ablates the size restriction inherent in ATAC-seq and will open up research in a variety of cell types in numerous other disease states. This award, under the East Asia and Pacific Summer Institutes program, supports summer research by a U.S. graduate student and is jointly funded by NSF and the Japan Society for the Promotion of Science.

深入了解衰老的分子基础是开发诊断和治疗方法以缓解年龄相关疾病的关键。然而，在创造一个通用的年龄灵丹妙药的困难之一是，一个有机体内的每个细胞的年龄不同。因此，衰老研究的目标之一是表征单个细胞如何随时间变化。 该项目将重点关注肌肉细胞，因为肌肉无力是老年人的常见症状。 单细胞研究通常是困难的，因为源材料的数量很少。测序和细胞收集技术的进步为研究单细胞基因组提供了机会。本研究项目利用横滨理化学研究所的设施，利用最新的单细胞技术，并受益于阿基博士的领导。本研究项目的目的是开发一种用于表征小鼠骨骼肌表观遗传异质性的单核转座酶可降解染色质测序（snATAC-seq）方案。虽然单细胞ATAC-seq是可用的，但可行的snATAC-seq仍未开发。ATAC-seq是一种确定表观遗传染色质修饰状态的新方法。与其他形式的测序不同，ATAC-seq能够映射核小体位置，从而提供细胞表观基因组的多维描述。将这一有前途的技术应用于老龄化和与老龄化有关的疾病问题，开辟了新的讨论和可能的治疗途径。单核ATAC-seq的开发消除了ATAC-seq固有的大小限制，并将在许多其他疾病状态中开辟各种细胞类型的研究。该奖项是东亚和太平洋夏季研究所计划下的一个奖项，由美国国家科学基金会和日本科学促进会共同资助，支持美国研究生的夏季研究。