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RUI: Architecture and evolution of a conserved bacterial regulatory pathway that influences host and microbe carbon metabolism

RUI：影响宿主和微生物碳代谢的保守细菌调节途径的结构和进化

基本信息

批准号：
1715956
负责人：
Alexandra Purdy
金额：
$ 51.32万
依托单位：
Amherst College
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1715956&HistoricalAwards=false
关键词：
RUI Architecture evolution conserved bacterial

项目摘要

All plants and animals exist in close partnership with communities of microbes, a relationship that is typically highly beneficial but can, in certain circumstances, lead to disease. Microbial communities provide to their hosts nutrients as well as small organic molecules that can function as molecular signals. These molecules generated by microbes are important to the normal development and physiological function of the host animal. One class of such nutrients (and signals) produced by microbes is the short-chain fatty acids, of which acetate is a familiar example. This project involves collaboration between laboratories at Amherst College (Purdy group) and Northern Michigan University (Sharp group) focused on understanding the signaling mechanisms in two bacteria (Vibrio and Pseudomonas) that regulate the levels of acetate and thereby its function in mediating interactions with microbial communities and animal hosts. This project will provide authentic research experiences for undergraduate students (including minorities under-represented in science) in both a course-based setting as well as in the investigators' laboratories. Women and under-represented minority students will be recruited and supported by peer-mentoring networks, and by outreach through public engagement including elementary and middle-school students, and groups such as Girl Scouts; Girls, Inc.; the Black Student Union High School Program, and the Marquette Children's Museum.The project will establish how the signaling pathway initiated by CrbS in Vibrio and Pseudomonas senses and transmits information to The project will establish how the signaling pathway initiated by CrbS in Vibrio and Pseudomonas senses and transmits information to regulate acetyl CoA synthetase and thereby control the levels of short chain fatty acids. The role of the CrbS-dependent regulon will be investigated to understand its evolution and role in carbon metabolism and in other diverse cellular functions in bacteria.

所有植物和动物都与微生物群落密切合作，这种关系通常非常有益，但在某些情况下可能导致疾病。微生物群落为其宿主提供营养物质以及可充当分子信号的有机小分子。微生物产生的这些分子对于宿主动物的正常发育和生理功能很重要。微生物产生的一类营养物质（和信号）是短链脂肪酸，乙酸盐就是一个常见的例子。该项目涉及阿默斯特学院（Purdy 小组）和北密歇根大学（Sharp 小组）实验室之间的合作，重点是了解两种细菌（弧菌和假单胞菌）调节乙酸水平的信号机制，从而了解其在介导与微生物群落和动物宿主相互作用中的功能。该项目将为本科生（包括在科学领域代表性不足的少数群体）在基于课程的环境以及研究人员的实验室中提供真实的研究经验。女性和代表性不足的少数族裔学生将通过同伴辅导网络以及通过包括中小学生和女童子军等团体在内的公众参与进行外展来招募和支持；女孩公司；该项目将确定弧菌和假单胞菌中 CrbS 启动的信号通路如何感知和传递信息。该项目将确定弧菌和假单胞菌中 CrbS 启动的信号通路如何感知和传递信息以调节乙酰辅酶 A 合成酶，从而控制短链脂肪酸的水平。我们将研究 CrbS 依赖性调节子的作用，以了解其进化以及在碳代谢和细菌其他多种细胞功能中的作用。