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RUI: The transcriptional response to quantitative variation in RpoS concentration in E. coli

RUI：大肠杆菌中 RpoS 浓度定量变化的转录反应

基本信息

批准号：
1716794
负责人：
Daniel Stoebel
金额：
$ 32.8万
依托单位：
Harvey Mudd College
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1716794&HistoricalAwards=false
关键词：
RUI transcriptional response quantitative variation

项目摘要

This project develops a quantitative understanding of how bacteria turn their genes on and off in response to stresses. This project will provide undergraduate students with experience performing research in microbiology, molecular genetics, and genomics. In addition, the PI will provide research internships to high school students participating in an Upward Bound program. These students are from backgrounds underrepresented in science, and the internships will give these students a path towards careers in science. The project studies two major questions about how the transcription of genes in the E. coli genome varies in response to the concentration of the regulatory protein RpoS. First, the project will test the role of specific transcription factors (TFs) in altering how genes respond to RpoS levels. This will be done by RNA-seq studies of the response to RpoS levels in strains lacking certain TFs. These whole genome studies will be complemented by detailed manipulations of TF binding sites in specific promoters. Second, the research will characterize the dose-response relationship between RpoS and transcription, and its influence over a physiological transition. This research will be done by altering RpoS levels using an arabinose-inducible RpoS system, and then measuring the effect on transcription using RNA-seq.

这个项目发展了对细菌如何在压力下开启和关闭基因的定量理解。该项目将为本科生提供微生物学、分子遗传学和基因组学方面的研究经验。此外，PI还将为参加“向上跳跃”项目的高中生提供研究实习机会。这些学生的背景在科学领域的代表性不足，实习将为这些学生提供一条通往科学事业的道路。该项目研究了两个主要问题，即大肠杆菌基因组中基因的转录如何随着调控蛋白RpoS的浓度而变化。首先，该项目将测试特定转录因子（TFs）在改变基因对RpoS水平的反应中的作用。这将通过RNA-seq研究缺乏某些tf的菌株对RpoS水平的反应来完成。这些全基因组研究将通过对特定启动子中TF结合位点的详细操作加以补充。其次，研究将表征RpoS与转录之间的剂量-反应关系及其对生理转变的影响。本研究将通过使用阿拉伯糖诱导的RpoS系统改变RpoS水平，然后使用RNA-seq测量对转录的影响来完成。