Defining the shared transcriptional network underlying Toxoplasma extracellular stress and stage transition

定义弓形虫细胞外应激和阶段转变背后的共享转录网络

• 批准号: 10682134
• 负责人: Marc-Jan Gubbels
• 金额: $ 23.21万
• 依托单位: BOSTON COLLEGE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-06 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10682134
• 关键词: Acquired Immunodeficiency Syndrome; Acute; Automobile Driving; Back; Binding Sites; Biology; Carbon Dioxide; Cell Cycle; Cell Cycle Stage; Cells; Cellular Stress; Chronic; Clinical; Cluster Analysis; Communities; Congenital Abnormality; Cyst; DNA Binding; Data; Data Analyses; Data Set; Drug Targeting; Early identification; Encephalitis; Environment; Event; Exposure to; Fibroblasts; Genes; Genetic Transcription; Genotype; Human; Hypersensitivity; Immune; Immune response; Impairment; In Vitro; Individual; Infection; Interferon Type II; Knowledge; Life; Maps; Mediating; Modeling; Muscle Cells; Muscle Fibers; Neurons; Opportunistic Infections; Organ Transplantation; Parasites; Pathway interactions; Pharmaceutical Preparations; Phase; Physiological; Population; Positioning Attribute; Research; Resistance; Resources; Role; Stress; System; Time; Tissues; Toxoplasma; Toxoplasma gondii; Toxoplasmosis; Transcriptional Regulation; Validation; Work; biological adaptation to stress; cancer therapy; cell type; combinatorial; comparative; computational pipelines; computerized tools; experimental study; extracellular; foodborne infection; gene interaction; innovation; insight; knock-down; new therapeutic target; novel; novel therapeutic intervention; obligate intracellular parasite; programs; promoter; reference genome; response; single-cell RNA sequencing; stressor; transcription factor; transcriptome; transcriptome sequencing; transcriptomics

Summary Toxoplasma gondii is an obligate intracellular apicomplexan parasite causing severe opportunistic infections. Current drugs are prone to induce hypersensitivity, especially upon long-term use. Infection comprises a short, acute stage (tachyzoite) followed by a dormant stage (bradyzoites in tissue cysts) for the life of the host. However, an impaired or suppressed immune response (e.g., AIDS, organ transplant, cancer treatment) can lead to reactivation of a dormant infection that was acquired years ago, leading to clinical toxoplasmosis. Interference with the tachyzoite-bradyzoite interconversion is an attractive target for novel therapeutic strategies. A key factor in stage interconversion is the ability to respond to stress: the immune response ‘stress’ maintains the bradyzoite stage, but if it wanes, the parasite defaults back to the acute stage. Reprogramming toward the bradyzoite is facilitated by the competitive interplay of about 10 transcription factors (TFs) balancing sequential, cooperative, and opposing roles. The research team recently discovered that the TF set associated with bradyzoite differentiation overlaps by 2/3 with the TFs expressed in parasites exposed to the extracellular (e.c.) environment. This provided the first glimpse of how the stress response is integrated into the transcriptional program leading to the bradyzoite. Indeed, the transcriptome of e.c. and bradyzoite parasites shares many genes, which positions the extracellular stage in between tachyzoites and bradyzoites. Here, the stress-related transcriptional program will be further dissected by extending on this observation with innovative CUT&RUN, single cell expression profiling (scRNA-seq), and the application of a novel in vitro generated myotube bradyzoite differentiation system. Excitingly, this myotube system demonstrated that spontaneous bradyzoite differentiation occurs in this cell type, but to reach and maintain a mature bradyzoite state, an exogenous, alkaline stress needs to be applied. Hence, this provides the perfect platform to dissect the contribution of stress-related transcriptional regulation to bradyzoite maturation. In short, the transcriptional network of stress will be dissected by 1. identifying the genes controlled by the 4-6 TFs shared between e.c. stress and bradyzoite conversion through CUT&RUN experiments; 2. scRNA-seq of mixed cell populations representing the transcriptional transitions of extracellular stress over the span of 6 hrs, and myotube-induced bradyzoite differentiation with and without alkaline stress. An advanced computational pipeline will be established to model the transitional programs that will permit answers to several open questions, such as: is there indeed a two-step bradyzoite differentiation pathway, and what are the genes driving these?; is the myotube seen as a stress by the parasites or not?; is there a specific stage in the cell cycle when bradyzoite differentiation occurs (a G2-like cell cycle stage and the late G1 restriction-checkpoint have been proposed)? In addition, comparative mapping of stress responses under different conditions will provide insights in how stress is integrated at the distinct parasite stages to modulate the stage-specific transcriptional responses.

总结