EAGER: Iron-Virus Interactions in the Ocean

EAGER:海洋中铁与病毒的相互作用

基本信息

  • 批准号:
    1722761
  • 负责人:
  • 金额:
    $ 29.93万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-02-01 至 2020-01-31
  • 项目状态:
    已结题

项目摘要

Iron is an essential micronutrient for phytoplankton that is required for photosynthesis and respiration. Insufficient iron has been shown to limit phytoplankton growth in large regions of the surface ocean, and correspondingly, iron cycling is directly linked to carbon cycling in much of the marine environment. Nearly all iron in seawater (99%) exists as complexes with organic molecules called ligands, which govern the concentration of iron dissolved in the water and the bioavailability of that iron to phytoplankton. However, despite the importance of iron-binding organic ligands, their sources and identities are largely unknown. Viruses, the majority of which are phages (viruses that infect bacteria), are extremely abundant in seawater and are in the same size fraction as dissolved iron. Recent evidence that non-marine phages contain iron as part of their structures has led to the proposal that marine phages may represent a previously overlooked class of organic iron-binding ligands. This project is determining the contribution of marine phages to dissolved iron pools and culture phage-host systems in the laboratory to determine if phages utilize bacterial iron-uptake receptors for infection in the manner of a Trojan horse. As the first study to examine the biogeochemical impact of trace elements contained within the structure of highly abundant marine phage particles, successful completion of the proposed research will be transformative for biological and chemical oceanography and have far-reaching implications for other fields, including human health where iron availability plays an important role in microbial pathogenesis. This project contributes to the multidisciplinary training of a graduate student and postdoctoral researcher. Research results will be disseminated through scientific publications and presentations, and the public will be educated about linkages between viruses and ocean chemistry via a hands-on exhibit for the annual St. Petersburg Science Festival. Building upon evidence from non-marine model systems demonstrating the presence of iron ions in phage tail proteins and phage utilization of cell surface receptors for siderophore-bound iron, this project combines field and laboratory-based experiments to test the following three hypotheses regarding iron-virus interactions in the oceans: (1) Iron incorporated into phage tails originates from bacterial cell reserves, reducing the amount of iron available for remineralization upon lysis; (2) Phages constitute important iron-binding ligands in the oceans, accounting for a substantial portion of organically complexed colloidal dissolved iron; (3) Marine phages compete with siderophore-bound iron for uptake receptors on the bacterial cell surface and use iron in their tails as a Trojan horse for infection. Initial calculations predict that phages could account for up to 70% of the colloidal fraction of organically complexed dissolved iron in the surface ocean; therefore, this project is critical for advancing knowledge of trace-metal cycling as well as phage-host interactions. Additionally, if a portion of the cellular iron thought to be released from bacterial cells for remineralization following lysis is already incorporated into phage tails, then these findings will have significant implications for oceanic biogeochemical models. Through a combination of laboratory-based culture experiments and field sample measurements, this project could reveal the identity of a ubiquitous component of colloidal organic iron-binding ligands, modify the estimates of iron concentrations and species released through viral lysis, and potentially identify a novel receptor type for marine phage that may compete with the acquisition of siderophore-bound iron by host bacteria.
铁是浮游植物必需的微量营养素,是光合作用和呼吸作用所必需的。铁不足已被证明限制了海洋表面大片区域的浮游植物生长,相应地,铁的循环与大部分海洋环境中的碳循环直接相关。海水中几乎所有的铁(99%)都以被称为配位体的有机分子的络合物的形式存在,这些配位体控制着水中溶解的铁的浓度以及这些铁对浮游植物的生物有效性。然而,尽管铁结合的有机配体很重要,但它们的来源和身份在很大程度上是未知的。病毒,其中大多数是噬菌体(感染细菌的病毒),在海水中极其丰富,与溶解的铁的大小相同。最近有证据表明,非海洋噬菌体的结构中含有铁,这导致了海洋噬菌体可能代表了一类以前被忽视的有机铁结合配体。该项目正在实验室中确定海洋噬菌体对溶解铁池和培养噬菌体宿主系统的贡献,以确定噬菌体是否以特洛伊木马的方式利用细菌铁摄取受体进行感染。作为第一个研究高度丰富的海洋噬菌体颗粒结构中所包含的微量元素的生物地球化学影响的研究,这项拟议的研究的成功完成将对生物和化学海洋学产生变革,并对其他领域,包括在微生物发病机制中铁的可获得性起重要作用的人类健康具有深远的影响。该项目对研究生和博士后研究人员的多学科培养做出了贡献。研究成果将通过科学出版物和演示文稿传播,并将通过一年一度的圣彼得堡科学节的动手展览,教育公众有关病毒和海洋化学之间的联系。基于来自非海洋模式系统的证据,表明噬菌体尾部蛋白中存在铁离子,以及噬菌体表面受体对铁载体结合的铁的利用,该项目结合了现场和实验室的实验,以检验关于海洋中铁与病毒相互作用的以下三个假说:(1)进入噬菌体尾部的铁来自细菌细胞储备,减少了裂解时可用于再矿化的铁量;(2)噬菌体是海洋中重要的铁结合配体,占有机胶体溶解铁的很大一部分;(3)海洋噬菌体与铁载体结合,竞争细菌细胞表面的铁摄取受体,并利用尾巴中的铁作为感染的特洛伊木马。初步计算预测,噬菌体可能占表层海洋中有机络合溶解铁的胶体分数的70%;因此,该项目对于推进痕量金属循环以及噬菌体-宿主相互作用的知识至关重要。此外,如果被认为是在裂解后从细菌细胞中释放出来进行再矿化的细胞铁的一部分已经被并入噬菌体尾巴中,那么这些发现将对海洋生物地球化学模型产生重大影响。通过实验室培养实验和现场样品测量的结合,该项目可以揭示胶体有机铁结合配体中普遍存在的成分的身份,修改通过病毒裂解释放的铁浓度和物种的估计,并有可能确定一种新的海洋噬菌体受体类型,它可能与宿主细菌获取铁载体结合的铁竞争。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Kristen Buck其他文献

Rapid Diuresis Protocol Reduces Hospital Stay In Acute Heart Failure Without Increasing 30-day Adverse Outcomes
快速利尿方案可缩短急性心力衰竭住院时间且不增加 30 天不良结局
  • DOI:
    10.1016/j.cardfail.2024.10.112
  • 发表时间:
    2025-01-01
  • 期刊:
  • 影响因子:
    8.200
  • 作者:
    Mazin Habhab;Matthew Hollowell;Sherilyn Munoz;Paul Weber;Ryan Crane;Morgan Korn;Anastasia Bury;Hassan Saleh;Kristen Buck;Nicole Mitchell;Ashly Sweet;Sangjin Lee;Matthew Gonzalez
  • 通讯作者:
    Matthew Gonzalez
The effect of ocean acidification on Fe speciation across distinct regions of the Eastern North Pacific
海洋酸化对北太平洋东部不同区域铁形态的影响
  • DOI:
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Lise Artigue;Salvatore Caprara;Charles Trick;William P Cochlan;Shigenobu Takeda;Mark Wells;Kristen Buck
  • 通讯作者:
    Kristen Buck
CYSTATIN C IS AN INDEPENDENT RISK PREDICTOR FOR DEATH OR MYOCARDIAL INFARCTION IN PATIENTS WITH ST-ELEVATION MYOCARDIAL INFARCTION (STEMI) AS WELL AS IN NON-ST-ELEVATION ACUTE CORONARY SYNDROME (NSTE-ACS)
  • DOI:
    10.1016/s0735-1097(11)60999-6
  • 发表时间:
    2011-04-05
  • 期刊:
  • 影响因子:
  • 作者:
    Axel Åkerblom;Lars Wallentin;Agneta Siegbahn;Richard C. Becker;Andrzej Budaj;Kristen Buck;Jay Horrow;Steen Husted;Hugo Katus;Philippe Gabriel Steg;Robert F. Storey;Nils Åsenblad;Stefan K. James
  • 通讯作者:
    Stefan K. James

Kristen Buck的其他文献

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{{ truncateString('Kristen Buck', 18)}}的其他基金

Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes
合作研究:连接寡营养海岸边缘的铁和氮源:固氮和边界通量的作用
  • 批准号:
    2326719
  • 财政年份:
    2023
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Standard Grant
NSFGEO-NERC: Collaborative Research: Using Time-series Field Observations to Constrain an Ocean Iron Model
NSFGEO-NERC:合作研究:利用时间序列现场观测来约束海洋铁模型
  • 批准号:
    2310573
  • 财政年份:
    2022
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Standard Grant
Collaborative Research: U.S. GEOTRACE GP17-OCE and GP17-ANT: Characterizing iron-binding organic ligands in the Southern Ocean and implications for iron cycling in the global ocean
合作研究:美国 GEOTRACE GP17-OCE 和 GP17-ANT:南大洋铁结合有机配体的特征及其对全球海洋铁循环的影响
  • 批准号:
    2219551
  • 财政年份:
    2022
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Continuing Grant
Collaborative Research: U.S. GEOTRACE GP17-OCE and GP17-ANT: Characterizing iron-binding organic ligands in the Southern Ocean and implications for iron cycling in the global ocean
合作研究:美国 GEOTRACE GP17-OCE 和 GP17-ANT:南大洋铁结合有机配体的特征及其对全球海洋铁循环的影响
  • 批准号:
    2300915
  • 财政年份:
    2022
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Continuing Grant
Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes
合作研究:连接寡营养海岸边缘的铁和氮源:固氮和边界通量的作用
  • 批准号:
    2148836
  • 财政年份:
    2022
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Standard Grant
Collaborative Research: The Effect of Ocean Acidification on Fe Availability to Phytoplankton in Coastal and Oceanic Waters of the Eastern North Pacific
合作研究:海洋酸化对北太平洋东部沿海和海洋水域浮游植物铁有效性的影响
  • 批准号:
    1829753
  • 财政年份:
    2019
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Standard Grant
NSFGEO-NERC: Collaborative Research: Using Time-series Field Observations to Constrain an Ocean Iron Model
NSFGEO-NERC:合作研究:利用时间序列现场观测来约束海洋铁模型
  • 批准号:
    1829777
  • 财政年份:
    2018
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Standard Grant
Collaborative Research: Diatoms, Food Webs and Carbon Export - Leveraging NASA EXPORTS to Test the Role of Diatom Physiology in the Biological Carbon Pump
合作研究:硅藻、食物网和碳输出 - 利用 NASA EXPORTS 测试硅藻生理学在生物碳泵中的作用
  • 批准号:
    1756433
  • 财政年份:
    2018
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Standard Grant
Collaborative Research: Investigating Iron-inding Ligands in Southern Ocean Diatom Communities: The Role of Diatom-Bacteria Associations
合作研究:调查南大洋硅藻群落中的铁配体:硅藻-细菌协会的作用
  • 批准号:
    1443483
  • 财政年份:
    2015
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Standard Grant
Collaborative Research: US GEOTRACES Pacific Section: Measurement of the organic complexation of dissolved iron, copper and cobalt, and total dissolved cobalt
合作研究:美国GEOTRACES太平洋部分:溶解铁、铜和钴的有机络合以及总溶解钴的测量
  • 批准号:
    1441969
  • 财政年份:
    2014
  • 资助金额:
    $ 29.93万
  • 项目类别:
    Continuing Grant

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