MRI: Acquisition of a Shared Use Integrated Next-Generation Sequencing Platform

授予蒙特克莱尔州立大学（MSU）购买下一代测序（NGS）平台（Illumina MiSeq），以扩展和更新MSU、西顿霍尔大学、帕塞伊克县社区学院和卑尔根社区学院的共享基因组基础设施。NGS技术彻底改变了生物科学，到目前为止，在很大程度上，一直受到本科研究和教学机构的保护。收购MiSeq将立即改善所有相关机构的教学基础设施。MiSeq系统将有效地影响课堂和基于实地/实验室的研究项目中种族多样化的学生。密歇根州立大学的学生人数正以惊人的速度增长（每年9%）。随着学生人数的增加，MSU研究教师的数量也在增加。MiSeq将被纳入完善的生物课程，最终将制定一个新的NGS选修课，目标是本科生在职业生涯的早期。这些课程将有效地培养学生在实验室为基础的研究项目，在那里他们将学习实验设计，NGS的有效使用，分析，解释和科学论文格式的DNA数据集的传播。这些课程将有额外的好处，培训希望进入生物技术劳动力的学生，进一步加强经济。NGS认证研讨会将有助于促进参与者的职业发展，或在申请生物技术甚至研究生院的工作时为参与者提供优势。每年的NGS公开讲座将有助于教育整个社区，让他们了解NGS时代的可能性，希望能激励下一代未来的科学家。周边高中的未来科学家也将通过韦斯顿学者计划（高中生科学指导）获得灵感。MiSeq将支持所有相关机构的教师，学生和合作者正在调查的不断扩大的基于DNA的研究项目。这些项目都是多学科/跨学科的，涵盖生物学，医学，化学和生态学中的各个子学科。在不久的将来，这些项目包括：1）检查脊椎动物中功能性牙齿基因的进化丢失; 2）确定水母等凝胶状动物的饮食; 3）确定水母毒液基因对治疗人类疾病的潜在应用; 4）进一步研究玉米（玉米）的基因调控，玉米是一种重要的农业作物; 5）调查与全球两栖动物数量减少和大规模死亡事件有关的野生动物病原体的遗传变异和独特性; 6）描述多种土壤类型中微生物的生物多样性（例如有机土壤、污染土壤）; 7）描述新泽西溪流中大型无脊椎动物的生物多样性; 8）鉴定构成有害藻华的微生物，以便更好地管理这些藻华并保护饮用水和娱乐沃茨; 9）通过观察金属胁迫反应，进一步探索蓝藻有害藻华的病原体; 10）进一步开发并行计算软件，以生成系统发育树; 11）确定当导致淋巴丝虫病（象皮病）的寄生虫受到压力时，可以作为治疗目标的分子变化。

An award is made to Montclair State University (MSU) to purchase a Next Generation Sequencing (NGS) platform (Illumina MiSeq) to expand and update the shared-use genomic infrastructure of MSU, Seton Hall University, Passaic County Community College, and Bergen Community College. NGS technologies have revolutionized the biological sciences and until now have, for the most part, been sheltered from undergraduate research and teaching institutions. Acquisition of the MiSeq will immediately improve the teaching infrastructure of all involved institutions. The MiSeq system will in effect impact ethnically diverse students both in the classroom and in field/laboratory -based research programs. The student body at MSU is growing at an astonishingly fast rate (9%/year). With this increase in student body size, the number of MSU research faculty is likewise increasing. The MiSeq will be incorporated into well-established biology classes and ultimately a new NGS elective class will be formulated targeting undergraduates early on in their careers. These classes will effectively train students in laboratory-based research projects where they will learn experimental design, effective use of NGS, analysis, interpretation, and dissemination of the DNA data sets in scientific paper format. These classes will have the added benefit of training students wishing to enter into the biotechnology workforce further strengthening the economy. The NGS certification workshops will help advance the careers of participants or give attendees an advantage when applying for jobs in biotechnology or even graduate school. The annual NGS public talks will help to educate the community at large in regards to what is possible in the age of NGS hopefully inspiring the next generation of future scientists. Upcoming scientists in surrounding high schools will also gain inspiration through the Weston Scholars Program (high school student science mentoring).The MiSeq will support the ever-expanding DNA based research projects being investigated by the faculty, students, and collaborators at all involved institutions. These projects are both multi/inter-disciplinary covering various sub-disciplines within biology, medicine, chemistry, and ecology. In the immediate future, these projects include: 1) Examine the evolutionary loss of functional teeth genes across vertebrate animals; 2) Determine the diet of gelatinous animals such as jellyfish; 3) Identify jellyfish venom genes with potential applications to the treatment of human ailments; 4) Further investigate gene regulation in maize (corn), an agriculturally important crop; 5) Investigate the genetic variation and uniqueness among wildlife pathogens implicated in amphibian declines and mass mortality events worldwide; 6) Characterize the biodiversity of microbes in multiple soil types (e.g. organic soils, contaminated soils); 7) Characterize macroinvertebrate biodiversity in New Jersey streams; 8) Characterize the microorganisms making up harmful algal blooms in order to better manage these blooms and protect drinking and recreational waters; 9) Further explore causative agents of cyanobacterial harmful algal blooms by looking at the metal stress response; 10) Further develop parallel computing software to generate phylogenetic trees; 11) Identify molecular changes that could be targeted for treatment when the parasite responsible for lymphatic filarialsis (elephantiasis) undergoes stress.