MRI: Acquisition of a Laser Confocal Microscope System for a Multi-User Core Imaging Facility Supporting Research and Training in the Sciences

MRI：为支持科学研究和培训的多用户核心成像设施采购激光共焦显微镜系统

• 批准号: 1726859
• 负责人: David Sharlin
• 金额: $ 57.52万
• 依托单位: Minnesota State University, Mankato
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726859&HistoricalAwards=false
• 关键词: MRI; Acquisition; Laser; Confocal; Microscope

An award is made to the Department of Biological Sciences at Minnesota State University Mankato (MSU), a primarily undergraduate institution, to acquire a Zeiss LSM880 with Airyscan Laser Scanning Confocal Microscope. The confocal microscope will immediately enhance MSU's motivated and well-trained faculty to conduct cutting edge research and train undergraduate and masters students in qualitative and quantitative fluorescent imaging. Additionally, the confocal microscope acquisition boosts MSU?s ability to recruit top-notch new faculty while facilitating current faculty's capacity to conduct transformative research, obtain grant funding, and publish results in high-quality journals. Importantly, it provides undergraduate and graduate students direct exposure to state-of-the-art confocal imaging technology necessary for future careers in the biological sciences through independent research projects and in-class utilization. Lastly, the confocal microscope will strengthen STEM/STEAM outreach activities of underrepresented groups through collaboration with already in-place college supported programs that aim to promote access to and interest in STEM for underrepresented groups.The acquired confocal microscope augments a wide range of research projects associated with several sciences disciplines, including biology, chemistry, and geology, by eliminating the limits imposed by conventional light microscopes on imaging and analysis. The confocal microscope strengthens the research programs of at least 13 investigators (PI, CoPI, 6 major users, and 5 auxiliary users) by elevating research projects that investigate tissue architecture, biochemical and cellular interactions, developmental biology, neurobiology, endocrinology, microbiology, plant science, and geology. Investigator projects' will utilize the full range of the confocal microscope's capabilities including high-resolution multi-label imaging, 3D reconstruction, live-imaging, and spectral unmixing. These projects span a multitude of model systems including rodents (mice), cell cultures lines, Anolis carolinensis (green anoles), Dictyostelium discoideum (slime mold), plant tissues, microorganisms, and sediments. The acquired confocal microscope is anticipated to not only meet the needs of current faculty, but also have the ability to meet the future needs of the department and university.

授予明尼苏达州立大学曼卡托（MSU）生物科学系一个主要的本科院校，以获得带有Airyscan激光扫描共聚焦显微镜的Zeiss LSM 880。共聚焦显微镜将立即提高密歇根州立大学的积极性和训练有素的教师进行尖端研究和培训本科生和硕士生在定性和定量荧光成像。此外，共聚焦显微镜采集提高MSU？的能力，招聘一流的新教师，同时促进当前教师的能力进行变革性的研究，获得赠款资金，并在高质量的期刊上发表成果。重要的是，它为本科生和研究生提供了直接接触到最先进的共聚焦成像技术，通过独立的研究项目和课堂利用，这是未来生物科学职业所必需的。最后，共聚焦显微镜将加强STEM/STEAM推广活动的代表性不足的群体，通过与已经到位的大学支持的计划，旨在促进访问和兴趣的STEM代表性不足的群体。收购的共聚焦显微镜扩大了广泛的研究项目与几个科学学科，包括生物学，化学和地质学，消除了传统光学显微镜对成像和分析的限制。共聚焦显微镜通过提升研究组织结构、生物化学和细胞相互作用、发育生物学、神经生物学、内分泌学、微生物学、植物科学和地质学的研究项目，加强了至少13名研究人员（PI、CoPI、6名主要用户和5名辅助用户）的研究计划。研究者项目将利用共焦显微镜的全方位功能，包括高分辨率多标记成像，3D重建，实时成像和光谱解混。这些项目涉及多种模型系统，包括啮齿动物（小鼠）、细胞培养系、Anolis carolinensis（绿色anoles）、Dictyosteelium discoideum（黏菌）、植物组织、微生物和沉积物。所获得的共聚焦显微镜预计不仅能满足当前教师的需求，而且有能力满足部门和大学的未来需求。

项目成果

The polyglutamine protein FAM171B localizes to neuronal cytoplasm

多聚谷氨酰胺蛋白 FAM171B 定位于神经元细胞质

• 发表时间: 2018
• 期刊: Abstracts - Society for Neuroscience
• 作者: RAJAGURU, D;BAUER, M;SHARLIN, D.S.
• 通讯作者: SHARLIN, D.S.

The Effects of Hexavalent Chromium on Dictyostelium Chemotaxis and Proteome

六价铬对盘基网柄菌趋化性和蛋白质组的影响

• 发表时间: 2019
• 期刊: The FASEB journal
• 作者: Daniel, Hanix Emeral;Moen, Rebecca
• 通讯作者: Moen, Rebecca

Ectopic brain-derived insulin-like growth factor-1 partially rescues neuroanatomical defects associated with developmental hypothyroidism

异位脑源性胰岛素样生长因子-1 可部分挽救与发育性甲状腺功能减退症相关的神经解剖学缺陷

• 发表时间: 2018
• 期刊: Abstracts - Society for Neuroscience
• 作者: Alexandra, Grond;Saatman, Kathryn;Sharlin, David S.
• 通讯作者: Sharlin, David S.

The Novel Protein FAM171B is recruited into Intracellular PolyQ Aggregates

新型蛋白质 FAM171B 被招募到细胞内 PolyQ 聚集体中

• 发表时间: 2019
• 期刊: Annual meeting of the Society for Neuroscience
• 作者: Kirlin, Justin;Goellner, Geoffrey
• 通讯作者: Goellner, Geoffrey

Localization and quanti cation of kisspeptin and gonadotropin inhibitory hormone in green anole lizards (anolis carolinensis)

绿变色龙蜥蜴 (anolis carolinensis) 中 Kisspeptin 和促性腺激素抑制激素的定位和定量

• 发表时间: 2018
• 期刊: Abstracts - Society for Neuroscience
• 作者: TALWAR, S;COHEN, R.E.
• 通讯作者: COHEN, R.E.