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Enhancing Research and Curriculum at California State University Dominguez Hills Through the Acquisition of Next-Generation Sequencing Instrumentation

通过购买下一代测序仪器加强加州州立大学多明格斯山分校的研究和课程

基本信息

批准号：
1727074
负责人：
Karin Kram
金额：
$ 21.63万
依托单位：
California State University-Dominguez Hills Foundation
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2021-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1727074&HistoricalAwards=false
关键词：
Enhancing Research Curriculum California State

项目摘要

An award is made to California State University Dominguez Hills (CSUDH) to acquire an Illumina MiSeq next-generation sequencer. The addition of this instrument to this campus will enhance research and teaching at the Hispanic-serving, Minority-serving institution. With CSUDH's increased commitment to cutting-edge research, the MiSeq will be the cornerstone of a plan to build infrastructure and capacity in the biosciences. The primary users will employ the MiSeq to study topics including bacterial evolution, environmental microbiology, developmental biology, and plant evolutionary genetics. The primarily low-income, Hispanic and African American undergraduate and graduate students who perform research in the faculty members' labs will use the current industry-standard MiSeq technology to answer these broad biological questions. These and other faculty members will incorporate authentic research experiences using the MiSeq into the courses they teach, potentially reaching hundreds of students per year. Additionally, CSUDH faculty will work with faculty from the local high-poverty K-12 school district and community colleges to include the MiSeq and genomic data in their curricula to build a bridge to biology careers. The increased interaction of faculty and students across these institutions will strengthen the educational pipeline and help these underserved students visualize successful careers as scientists.The on-campus capabilities of CSUDH's team of new, research-driven investigators in the biosciences will be dramatically enhanced by the acquisition of a MiSeq. Initially, three research groups and five research projects will use the next generation sequencing technology. The MiSeq has the potential to assist faculty researchers in making contributions in the following areas: characterizing the effects of environment and genotype on microbial evolution, determining the role the nectar microbiome plays in pollinator preferences, identifying both the genetic and microbial factors that contribute to neuron development and function in zebrafish, and predicting the effects of climate change on plant evolution. This equipment will greatly increase the research productivity of faculty and student researchers, allowing the researchers to easily and cost-effectively use techniques such as metagenomic sequencing, bacterial whole genome re-sequencing, transcriptomics, targeted sequencing, and genotyping-by-sequencing. Further, the addition of this instrument to CSUDH facilities will help catapult the institution into a higher tier of research institutions, increasing opportunities for faculty and students.

加州州立大学多明格斯山庄(CSUDH)获得了一台Illumina MiSeq下一代测序仪。这一工具加入这个校园将加强这个为拉美裔和少数族裔服务的机构的研究和教学。随着南加州大学越来越多地致力于尖端研究，MiSeq将成为建设生物科学基础设施和能力的计划的基石。主要用户将使用MiSeq学习包括细菌进化、环境微生物学、发育生物学和植物进化遗传学在内的主题。在教师实验室进行研究的主要是低收入、西班牙裔和非裔美国本科生和研究生，他们将使用当前行业标准的MiSeq技术来回答这些广泛的生物学问题。这些和其他教职员工将把使用MiSeq的真实研究体验融入到他们教授的课程中，每年可能接触到数百名学生。此外，南加州大学的教职员工将与当地高贫困的K-12学区和社区大学的教职员工合作，将MiSeq和基因组数据纳入他们的课程，以建立通往生物学职业生涯的桥梁。这些机构的教职员工和学生之间的互动增加将加强教育渠道，并帮助这些服务不足的学生将成功的职业生涯视作科学家。CSUDH的新的、以研究为导向的生物科学研究团队的校园能力将通过收购MiSeq而显著增强。最初，三个研究小组和五个研究项目将使用下一代测序技术。MiSeq有可能在以下领域帮助教师研究人员做出贡献：表征环境和基因对微生物进化的影响，确定花蜜微生物群在传粉者偏好中所起的作用，识别有助于斑马鱼神经元发育和功能的遗传和微生物因素，以及预测气候变化对植物进化的影响。这种设备将极大地提高教职员工和学生研究人员的研究效率，使研究人员能够轻松且经济高效地使用诸如元基因组测序、细菌全基因组重测序、转录组、靶向测序和按测序进行基因分型等技术。此外，将这一仪器添加到CSUDH设施将有助于将该机构推向更高层次的研究机构，增加教职员工和学生的机会。