Collaborative Proposal: Selection and Genetic Succession in the Intertidal -- Population Genomics of Pisaster ochraceus During a Wasting Disease Outbreak and its Aftermath
合作提案:潮间带的选择和遗传演替——消耗性疾病爆发及其后果期间 Pisaster ochraceus 的群体基因组学
基本信息
- 批准号:1737091
- 负责人:
- 金额:$ 18.42万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-12-01 至 2024-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This project seeks to understand the outcomes of predator-disease dynamics by exploring a recent pandemic that decimated 90% of ochre sea stars (Pisaster ochraceus) in the eastern North Pacific in 2013. The research team will explore how recovery may depend upon often difficult-to-see processes such as the interplay of migration and natural selection in marine species. While the population of sea stars is currently rebounding due to several years of unusually high recruitment, the sea star wasting disease continues to persist at low levels. This project aims to determine the genetic consequences of the pandemic and subsequent recovery. The team will determine whether the majority of susceptible sea stars have died and identify possible refuges where susceptible sea stars survived. They will examine the potential for heritable variation in resistance to this disease in order to assess whether the new recruits are tolerant or susceptible to wasting. Resolving these issues will enable predictions about the trajectory of their recovery and the potential responses to future large scale disease outbreaks. Research findings will be shared with resource managers and scientists at a collaborative workshop that will focus on state-of-the-art methods to advance research on marine diseases. The public will have the opportunity to learn more about sea star wasting disease through a partnership with the UCSC Seymour Marine Discovery Center and can track the incidence of disease using an online interactive map available at www.seastarwasting.org. Results will be incorporated into professional development for teachers with CalTeach and adapted for teaching materials up to college-level. This project will train diverse early career scientists - undergraduates, graduates, and a postdoctoral scholar - in integration of ecological and genomic methods.Understanding the consequences of large-scale pandemics in the broader contexts of geographic heterogeneity and chronic changes in ocean pH and temperature is an emerging contemporary issue. This project employs long-term characterization of population dynamics and genetic consequences of a sea star wasting disease (SSWD) outbreak, which caused median 90% mortality in Pisaster ochraceus populations in the northeastern Pacific, to estimate potential long-term consequences for the species. While the largest recorded influx of new recruits occurred in 2014-2016, it is unknown where they originated from, whether recruits and surviving adults remained susceptible to the disease, which persisted at low levels, and for how long these dynamics might continue. This long-term dataset provides a unique opportunity for exploring the short and long term repercussions of such large-scale disease outbreaks and the population dynamics that they precipitate. This project builds on long-term field studies of wild populations to describe host population dynamics, the disease, and genomic diversity. The goal is to discover genetic variation associated with SSWD and to dissociate that variation from population genomic effects attributable to abiotic environmental variation. Objectives are: (1) Census P. ochraceus at 24 sites throughout its range to describe population dynamics, the prevalence of SSWD, and measure abiotic variables. (2) Conduct laboratory experiments coupled with RNAseq analyses to determine loci differentially regulated during exposure to SSWD, temperature, salinity, and pCO2 anomalies. (3) Map ddRAD, RNAseq, and candidate loci under selection to a P. ochraceus genome. (4) Conduct range-wide population genomic analyses for 3 years to assess genetic (SNP) variation among wild-caught specimens with, versus without, SSWD across a geographic mosaic of abiotic variation. (5) Explore links between SSWD and candidate loci, such as EF1A. These analyses will describe the immediate genomic consequences of the disease outbreak, the population dynamics that the outbreak set in motion, and the interplay of factors and mechanisms - such as disease, temperature, migration, selection - that affected these changes. The results will advance understanding of general processes and interactions that shape population genomic structure in coastal ecosystems, providing resources to inform future research and applications in design of management strategies for coastal living resources.
该项目旨在通过探索2013年在北太平洋东部毁灭90%赭色海星(Pisaster ochraceus)的最近大流行来了解捕食者疾病动态的结果。研究小组将探讨恢复如何取决于通常难以看到的过程,例如海洋物种的迁移和自然选择的相互作用。虽然由于几年来异常高的招募,海星的数量目前正在反弹,但海星星星消耗病继续保持在低水平。该项目旨在确定大流行病的遗传后果和随后的恢复。研究小组将确定大多数易感海星是否已经死亡,并确定易感海星幸存的可能避难所。他们将研究对这种疾病的抵抗力的遗传变异的可能性,以评估新招募的新兵是否耐受或容易消瘦。解决这些问题将能够预测其恢复的轨迹以及对未来大规模疾病爆发的潜在反应。研究结果将在一个合作讲习班上与资源管理人员和科学家分享,该讲习班将重点讨论推进海洋疾病研究的最新方法。公众将有机会通过与UCSC西摩海洋发现中心的合作了解更多关于海星星消耗病的信息,并可以使用在线互动地图跟踪疾病的发生率,该地图可在www.seastarwasting.org上获得。结果将被纳入CalTeach教师的专业发展,并适用于大学水平的教材。该项目将培养不同的早期职业科学家-本科生,研究生和博士后学者-在生态学和基因组学方法的整合。了解在地理异质性和海洋pH值和温度的慢性变化的更广泛的背景下,大规模流行病的后果是一个新兴的当代问题。该项目采用了长期的人口动态和遗传后果的表征的海星星消耗病(SSWD)爆发,造成中位值90%的死亡率在Pisaster赭色种群在东北太平洋,估计潜在的长期后果的物种。虽然有记录以来最大规模的新兵涌入发生在2014年至2016年,但目前尚不清楚他们来自哪里,新兵和幸存的成年人是否仍然容易感染这种疾病,这种疾病在低水平上持续存在,以及这种动态可能持续多久。这一长期数据集为探索此类大规模疾病爆发的短期和长期影响及其引发的人口动态提供了独特的机会。该项目建立在对野生种群的长期实地研究的基础上,以描述宿主种群动态、疾病和基因组多样性。目的是发现与SSWD相关的遗传变异,并将该变异与非生物环境变异引起的群体基因组效应分离。目标是:(1)在整个分布区的24个地点进行普查,以描述种群动态,SSWD的患病率,并测量非生物变量。(2)进行实验室实验结合RNAseq分析,以确定在暴露于SSWD,温度,盐度和pCO 2异常期间差异调节的基因座。(3)将ddRAD、RNAseq和选择下的候选基因座定位到赭曲霉基因组。(4)进行范围广泛的人口基因组分析3年,以评估遗传(SNP)之间的变化,野生捕获的标本,与没有SSWD跨越地理镶嵌的非生物变异。(5)探索SSWD与候选基因座(如EF 1A)之间的联系。 这些分析将描述疾病爆发的直接基因组后果,爆发引发的种群动态,以及影响这些变化的因素和机制(如疾病,温度,迁移,选择)的相互作用。研究结果将促进对沿海生态系统中塑造种群基因组结构的一般过程和相互作用的理解,为未来的研究和沿海生物资源管理策略设计应用提供信息资源。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Is It in the Stars? Exploring the Relationships between Species’ Traits and Sea Star Wasting Disease
- DOI:10.1086/722800
- 发表时间:2022-12
- 期刊:
- 影响因子:0
- 作者:L. M. Schiebelhut;Melina Giakoumis;R. Castilho;Valentina E. Garcia;J. Wares;G. Wessel;M. Dawson
- 通讯作者:L. M. Schiebelhut;Melina Giakoumis;R. Castilho;Valentina E. Garcia;J. Wares;G. Wessel;M. Dawson
Evidence of introgression, ecological divergence and adaptation in Asterias sea stars
- DOI:10.1111/mec.17118
- 发表时间:2023-09
- 期刊:
- 影响因子:4.9
- 作者:Melina Giakoumis;G. Pinilla‐Buitrago;Lukas J. Musher;J. Wares;S. J. Baird;M. Hickerson
- 通讯作者:Melina Giakoumis;G. Pinilla‐Buitrago;Lukas J. Musher;J. Wares;S. J. Baird;M. Hickerson
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John Wares其他文献
John Wares的其他文献
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{{ truncateString('John Wares', 18)}}的其他基金
Collaborative Research: Evaluating Marine Clines to Predict Larval Retention
合作研究:评估海洋环境以预测幼虫滞留
- 批准号:
1029526 - 财政年份:2010
- 资助金额:
$ 18.42万 - 项目类别:
Standard Grant
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Collaborative Proposal: Selection and Genetic Succession in the Intertidal - Population Genomics of Pisaster ochraceus During a Wasting Disease Outbreak and its Aftermath
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