Dimensions: Collaborative Research: Elucidating the drivers of mutualism variation in host-symbiont metapopulations

维度：合作研究：阐明宿主-共生复合种群中互利共生变异的驱动因素

• 批准号: 1738028
• 负责人: Jeffrey Chang
• 金额: $ 35万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-10-01 至 2022-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1738028&HistoricalAwards=false
• 关键词: Dimensions; Collaborative; Research; Elucidating; drivers

Symbiotic bacteria transform how plants and animals interact with their environment. These bacteria are well known for their ability to enhance host growth and tolerance to stress, but symbioses vary greatly in their effects on host health and fitness. Little is understood about the forces that shape this variation and drive the spread of symbionts that interact, but fail to benefit the host. Here, researchers use the relationship between native California legumes and nitrogen-fixing Bradyrhizobium bacteria to study the drivers of variation in symbioses. The project will employ environmental sampling of interacting plants, bacteria, and soil, genetic approaches, and greenhouse experiments. The research will determine the magnitude of benefits the bacteria provide to the host, what bacterial genes facilitate benefit or exploitation of the host, and how the host responds and defends itself against ineffective symbionts. The project will train undergraduate and graduate students as well as two postdoctoral fellows. The researchers will educate local farmers on plant-microbe interactions, and they will generate and curate a collection of plant and bacterial variants that will be made freely available to other researchers. The project is important because it will provide information on the parameters that influence symbiosis and help guide how microbes can be better deployed to increase productivity of agricultural systems and promote health of humans and the planet. The maintenance of diversity in microbial symbioses is paradoxical. Conventional theory predicts a lack of variation in symbiont function because host species reward beneficial symbionts and intensely select against ineffective partners. Here, a novel framework is tested to explain the maintenance of symbiont variation. The planned work transcends the dominant theoretical paradigm by investigating ecological, spatiotemporal, and genomic drivers in a metapopulation of rhizobial bacteria and their native legume hosts. Like other hosts, legumes exhibit host control traits: legumes can discriminate against ineffective rhizobia during nodule formation, and can reduce within-nodule growth rates of ineffective rhizobia. Despite the apparent efficiency of host control, ineffective rhizobia are commonly uncovered in natural and agricultural soils. Four aims are proposed to explain the maintenance of symbiont variation in this key model system. Experimental inoculations coupled with genetic and selection analysis are used to investigate i) variation among legume host populations in their capacity to sanction ineffective rhizobia, ii) the spatial and phylogenetic origins of invasions by ineffective rhizobia, and iii) the genomic basis for the evolution of ineffective rhizobia. iv) Inferred invasions of ineffective rhizobia will be recapitulated using experiments that test fitness predictions under realistic conditions. This work challenges conventional mutualism theory and highlights the importance of genetic variation, spatio-temporal dynamics, and fitness tradeoffs.

共生细菌改变了植物和动物与环境的相互作用。这些细菌以其增强宿主生长和对压力的耐受性的能力而闻名，但共生体对宿主健康和健身的影响差异很大。人们对形成这种变异的力量以及推动共生体传播的力量知之甚少，这些共生体相互作用，但无法使宿主受益。在这里，研究人员利用本地加州豆类和固氮慢生根瘤菌之间的关系来研究共生体变化的驱动因素。该项目将采用相互作用的植物，细菌和土壤，遗传方法和温室实验的环境采样。这项研究将确定细菌为宿主提供的益处的大小，哪些细菌基因促进宿主的益处或利用，以及宿主如何应对和保护自己免受无效共生体的侵害。该项目将培训本科生和研究生以及两名博士后研究员。研究人员将教育当地农民了解植物与微生物的相互作用，他们将产生和策划一系列植物和细菌变体，这些变体将免费提供给其他研究人员。该项目很重要，因为它将提供有关影响共生的参数的信息，并帮助指导如何更好地部署微生物，以提高农业系统的生产力，促进人类和地球的健康。维持微生物共生体的多样性是自相矛盾的。传统理论预测共生体功能缺乏变化，因为宿主物种奖励有益的共生体，并强烈选择无效的伴侣。在这里，一个新的框架进行测试，以解释共生体的变化的维护。计划的工作超越了主导的理论范式，通过调查生态，时空和基因组驱动程序的根瘤菌和他们的原生豆类宿主的集合种群。与其他寄主一样，豆科植物也表现出寄主控制特性：在根瘤形成过程中，豆科植物可以区别对待无效根瘤菌，并可以降低无效根瘤菌的根瘤内生长速率。尽管根瘤菌对宿主的控制有明显的效果，但在自然土壤和农业土壤中通常发现无效的根瘤菌。提出了四个目标来解释共生体变异在这个关键模型系统中的维持。实验接种加上遗传和选择分析被用来调查i）豆类宿主种群之间的变化，在他们的能力制裁无效根瘤菌，ii）无效根瘤菌入侵的空间和系统发育起源，和iii）无效根瘤菌进化的基因组基础。iv）无效根瘤菌的推断入侵将使用在现实条件下测试适合度预测的实验来概括。这项工作挑战了传统的互惠理论，并强调了遗传变异，时空动态和健身权衡的重要性。

项目成果

Recurrent mutualism breakdown events in a legume rhizobia metapopulation

• DOI: 10.1098/rspb.2019.2549
• 发表时间: 2020-01-29
• 期刊: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
• 影响因子: 4.7
• 作者: Gano-Cohen, Kelsey A.;Wendlandt, Camille E.;Sachs, Joel L.
• 通讯作者: Sachs, Joel L.

Evolution of specialization in a plant‐microbial mutualism is explained by the oscillation theory of speciation

物种形成振荡理论解释了植物的专业化进化——微生物互利共生

• DOI: 10.1111/evo.14222
• 发表时间: 2021
• 期刊: Evolution
• 影响因子: 3.3
• 作者: Torres‐Martínez, Lorena;Porter, Stephanie S.;Wendlandt, Camille;Purcell, Jessica;Ortiz‐Barbosa, Gabriel;Rothschild, Jacob;Lampe, Mathew;Warisha, Farsamin;Le, Tram;Weisberg, Alexandra J.
• 通讯作者: Weisberg, Alexandra J.