Molecular mechanisms for signal-receptor functional coupling in pheromonal systems

信息素系统中信号-受体功能耦合的分子机制

• 批准号: 1754264
• 负责人: Yehuda Ben-Shahar
• 金额: $ 77万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-15 至 2025-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754264&HistoricalAwards=false
• 关键词: Molecular; mechanisms; signal; receptor; functional

Successful mating is essential for the survival of most animal species. For many species, the decision with whom to mate depends on information communicated via the release of 'pheromones', a complex blend of chemicals that are released by one animal to be sensed by another. Therefore, understanding how pheromones affect mating behaviors has broad implications for animal behavior and evolutionary biology. Because pheromones communicate precise information, the links between pheromone production and perception are expected to be relatively stable within species. However, previous studies suggest that pheromones are remarkably flexible, and can change rapidly as new species evolve. One possible explanation for this apparent puzzle is that some shared genes co-regulate the independent processes responsible for pheromone production and perception. These shared genes could provide a path for the evolution of pheromone signals without losing the link between pheromone production and perception. This hypothesis will be tested by taking advantage of the fruit fly courtship ritual as a model. Studies will focus on genes that encode proteins that function as pheromone receptors in the nervous system, as well as regulators of pheromone cuticular production, and their possible role in pheromonal differences between males and females and across related fruit fly species. In addition to training opportunities for high school and undergraduate students, the broader impacts of this project will include genetics and neuroscience outreach and education activities associated with the public education system in the St. Louis region. Mating pheromones are essential for the maintenance of species boundaries in diverse animal taxa. However, it is puzzling how, as closely related species diversify, pheromones could evolve without fitness costs associated with their decoupling from related mate choice behaviors. The goal of this project is to test the hypothesis that mating pheromone production and perception are genetically linked via the action of pleiotropic genes. Specifically, the project will test the hypothesis that members of the Gustatory receptor protein family in Drosophila play a role in the sensory perception of specific cuticular pheromones as well as in the regulation of their synthesis by acting as regulatory autoreceptors in the pheromone-producing oenocytes. Approaches will include a combination of behavioral, chemo-analytical, and state-of-the-art CRISPR/Cas9-dependent genome editing approaches to establish a genetic linkage between the perception and production of pheromones, to investigate the role of pleiotropic chemoreceptors in regulating the female pre-to-post-mating phenotypic switch from attractive to repulsive pheromonal profile, and the role of pheromone receptor evolution in promoting assortative mating and speciation in the genus Drosophila. Findings will provide novel mechanistic insights into the regulation of mating behaviors by pheromones in particular, and the evolution of mating signals in general. In addition to diverse genetics and neuroscience outreach activities in the St. Louis region, the investigator and co-workers will use studies of mating behaviors as training opportunities for high school and undergraduate students in experimental design, behavioral and genetic data collection and analyses, and scientific presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

成功的交配对大多数动物物种的生存至关重要。对于许多物种来说，与谁交配的决定取决于通过释放“信息素”传递的信息，信息素是一种由一种动物释放的化学物质的复杂混合物，可以被另一种动物感觉到。因此，了解信息素如何影响交配行为对动物行为和进化生物学具有广泛的意义。由于信息素传递精确的信息，信息素的产生和感知之间的联系预计在物种内相对稳定。然而，以前的研究表明，信息素是非常灵活的，可以随着新物种的进化而迅速变化。对这个明显的谜团的一个可能的解释是，一些共享的基因共同调节负责信息素产生和感知的独立过程。这些共享的基因可以为信息素信号的进化提供一条路径，而不会失去信息素产生和感知之间的联系。这一假设将通过利用果蝇求偶仪式作为模型来检验。研究将集中在编码蛋白质的基因，这些蛋白质在神经系统中起信息素受体的作用，以及信息素表皮生产的调节剂，以及它们在雄性和雌性之间以及相关果蝇物种之间信息素差异中的可能作用。除了为高中和本科生提供培训机会外，该项目的更广泛影响将包括与圣路易斯地区公共教育系统相关的遗传学和神经科学宣传和教育活动。交配信息素是维持不同动物类群物种界限的必要条件。然而，令人困惑的是，如何密切相关的物种多样化，信息素可以进化没有健身成本与相关的配偶选择行为脱钩。这个项目的目标是测试的假设，交配信息素的生产和感知是通过多效性基因的行动遗传联系。具体而言，该项目将测试的假设，即在果蝇的味觉受体蛋白质家族的成员在特定的表皮信息素的感官知觉，以及在其合成的调节中发挥作用，作为监管自动受体的信息素生产的酒细胞。方法将包括行为、化学分析和最先进的CRISPR/Cas9依赖性基因组编辑方法的组合，以建立信息素的感知和产生之间的遗传联系，研究多效性化学感受器在调节雌性交配前至交配后表型转换中的作用，信息素受体的进化在促进果蝇属的交配和物种形成中的作用。研究结果将为特别是信息素对交配行为的调节以及一般交配信号的进化提供新的机制见解。除了在圣路易斯地区开展各种遗传学和神经科学外展活动外，研究人员和同事还将利用交配行为的研究作为高中和本科生在实验设计、行为和遗传数据收集和分析方面的培训机会，该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准。

项目成果

The cuticular hydrocarbon profiles of honey bee workers develop via a socially-modulated innate process

• DOI: 10.7554/elife.41855
• 发表时间: 2019-02-05
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Vernier, Cassondra L.;Krupp, Joshua J.;Ben-Shahar, Yehuda
• 通讯作者: Ben-Shahar, Yehuda

The Drosophila ERG channel seizure plays a role in the neuronal homeostatic stress response

• DOI: 10.1371/journal.pgen.1008288
• 发表时间: 2019-08-01
• 期刊: PLOS GENETICS
• 影响因子: 4.5
• 作者: Hill, Alexis S.;Jain, Poorva;Ben-Shahar, Yehuda
• 通讯作者: Ben-Shahar, Yehuda

The Genetic Architecture of Larval Aggregation Behavior in Drosophila

• DOI: 10.1080/01677063.2021.1887174
• 发表时间: 2020-11
• 期刊: bioRxiv
• 作者: Ross M McKinney;Y. Ben-Shahar

The neurogenetics of sexually dimorphic behaviors from a postdevelopmental perspective

从后发育的角度研究性二态性行为的神经遗传学

• DOI: 10.1111/gbb.12623
• 发表时间: 2019
• 期刊: Brain and Behavior
• 影响因子: 3.1
• 作者: Leitner, Nicole;Ben‐Shahar, Yehuda
• 通讯作者: Ben‐Shahar, Yehuda

Brain microRNAs among social and solitary bees

• DOI: 10.1098/rsos.200517
• 发表时间: 2019-08
• 期刊: Royal Society Open Science
• 影响因子: 3.5
• 作者: K. Kapheim;Beryl M. Jones;Eirik Søvik;Eckart Stolle;Robert M. Waterhouse;G. Bloch;Y. Ben-Shahar