Synthesis and purification of bioactive flavonoids

作为研究联盟“FlavoNET”的合作伙伴，我们开发并改进了各种14 C标记的黄酮类化合物的合成。使用前体的部分化学合成的组合，从微生物系统中的植物来源的cDNA的异源表达获得的重组蛋白质和各种类黄酮的色谱纯化系统现在是可用的。在过去的项目期间，对关键步骤--柚皮素合成进行了优化。所获得的前体现在可以用于几种所需类黄酮的分步生化合成，例如染料木素、圣草酚、芹菜素或毛地黄黄酮。此外，我们使用转基因微生物（E科尔和面包酵母），以建立一个体内合成系统（生物转化），以精制特定的未标记的黄酮类化合物，但也建立了几个黄酮类化合物的总合成的前体衍生的苯丙烷途径。这两种方法都被证明是几个酶的步骤，也能够大量生产14 C标记的黄酮类化合物。

As partner of the research consortium "FlavoNET" we have developed and improved the synthesis of various 14C-labelled flavonoids. Using a combination of partial chemical synthesis of precursors, recombinant proteins obtained from heterologous expression of plant derived cDNA's in microbial systems and Chromatographie purification systems various flavonoids are available now. The key step - naringenin synthesis - was optimized during the past project time. The obtained precursor can be used now for stepswise biochemical synthesis of several requested flavonoids, e.g. genistein, eriodictyol, apigenin or luteolin. Moreover, we used transgenic microorganisms (E coll and bakers yeast) to establish an in-vivo synthesis system (biotransformation) to refine specific unlabelled flavonoids but also to establish total synthesis of several flavonoids from precursors derived from phenylpropanoid pathway. Both approaches were demonstrated for several enzymatic steps and were also capable for large production of 14C-labelled flavonoids.