Purification, structure characterization of bioactive substances from marine organisms and application them to the novel targeting oral cancer drugs

海洋生物活性物质的纯化、结构表征及其在口腔癌新型靶向药物中的应用

基本信息

批准号：
23592962
负责人：
YUKIO YOSHIOKA
金额：
$ 3.24万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

项目摘要

Marine organisms from Japanese adjacent Sea is collected and freeze-dried. They are homogenized in EtOH-MeOH(4:1)and distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer. Following it, the extracted compounds are investigated their activity by bio-assay and are purified and decided their structure by LC/MS. Their analogues improving the structure of their branches are produced and massively synthesized them. The anti-tumor activity is investigated by the inoculated tumor cell on nude mouse. CD133 is a useful marker of the oral squamous cell carcinoma cells. The oral cancer stem cells were isolated from the oral squamous cell carcinoma cell line with index of CD133 and analyzed the character of a stem cell. The mechanism of the novel anti-tumor agents against not only the oral cancer but also the oral cancer stem cells is analyzed in vitro.

收集并冷冻干燥的日本邻近海洋生物。它们在EtoH-MeOH（4：1）中均质，并通过HPLC分布到己烷层和C2H5OCH3CO层和水层中。之后，提取的化合物通过生物测定进行了研究，并通过LC/MS纯化并决定其结构。他们的类似物改善了分支的结构，并大量合成了它们。通过裸鼠的接种肿瘤细胞研究抗肿瘤活性。 CD133是口服鳞状细胞癌细胞的有用标记。用CD133指数从口腔鳞状细胞癌细胞系中分离出口腔癌干细胞，并分析干细胞的特征。在体外分析了新型抗肿瘤药物对口腔癌和口腔癌干细胞的机制。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

佩吉特骨病一例，其中双磷酸盐相关的颌骨坏死通过牺牲骨去除进行治疗，并且可以进行长期观察。

DOI：
发表时间：
2012
期刊：
日本口腔科学会雑誌
影响因子：
0
作者：
Tanaka T;Nakayama H;Yoshitake Y;Irie A;Nagata M;Kawahara K;Takamune Y;Yoshida R;Nakagawa Y;Ogi H;Shinriki S;Ota K;Hiraki A;Ikebe T;Nishimura Y;Shinohara M;吉岡幸男　虎谷茂昭　向笠英恵　神田拓　新谷智章　岡本哲治
通讯作者：
吉岡幸男　虎谷茂昭　向笠英恵　神田拓　新谷智章　岡本哲治

Petrosiols A-E, neurotrophic diyne tetraols isolated from the Okinawan sponge Petrosia strongylata