Purification, structure characterization of bioactive substances from marine organisms and application them to the novel targeting oral cancer drugs

海洋生物活性物质的纯化、结构表征及其在口腔癌新型靶向药物中的应用

• 批准号: 23592962
• 负责人: YUKIO YOSHIOKA
• 金额: $ 3.24万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592962/
• 关键词: 海洋生物; 生理活性物質; 抗腫瘍効果; 口腔がん; 癌幹細胞; 分子標的薬; 口腔癌; 口腔癌幹細胞; 分子標的治療薬; がん幹細胞

Marine organisms from Japanese adjacent Sea is collected and freeze-dried. They are homogenized in EtOH-MeOH(4:1)and distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer. Following it, the extracted compounds are investigated their activity by bio-assay and are purified and decided their structure by LC/MS. Their analogues improving the structure of their branches are produced and massively synthesized them. The anti-tumor activity is investigated by the inoculated tumor cell on nude mouse. CD133 is a useful marker of the oral squamous cell carcinoma cells. The oral cancer stem cells were isolated from the oral squamous cell carcinoma cell line with index of CD133 and analyzed the character of a stem cell. The mechanism of the novel anti-tumor agents against not only the oral cancer but also the oral cancer stem cells is analyzed in vitro.

收集日本邻近海域的海洋生物并冷冻干燥。它们在乙氧甲烷（4:1）中均质，通过高效液相色谱（HPLC）分布到己烷层、C2H5OCH3CO层和水层中。随后，对提取的化合物进行生物活性测定，并采用液相色谱/质谱法对其进行纯化和结构鉴定。它们的类似物改善了它们分支的结构，并被大量合成。用接种的肿瘤细胞对裸鼠进行抗肿瘤活性研究。CD133是口腔鳞状细胞癌的有效标志物。从具有CD133指数的口腔鳞癌细胞系中分离出口腔癌干细胞，并对其特性进行分析。分析了新型抗肿瘤药物对口腔癌和口腔癌干细胞的体外作用机制。

项目成果

ビスフォスフォネート関連顎骨壊死にて腐骨除去術を行い長期観察し得た骨Paget病の１例

佩吉特骨病一例，其中双磷酸盐相关的颌骨坏死通过牺牲骨去除进行治疗，并且可以进行长期观察。

• 发表时间: 2012
• 期刊: 日本口腔科学会雑誌
• 作者: Tanaka T;Nakayama H;Yoshitake Y;Irie A;Nagata M;Kawahara K;Takamune Y;Yoshida R;Nakagawa Y;Ogi H;Shinriki S;Ota K;Hiraki A;Ikebe T;Nishimura Y;Shinohara M;吉岡幸男 虎谷茂昭 向笠英恵 神田拓 新谷智章 岡本哲治
• 通讯作者: 吉岡幸男 虎谷茂昭 向笠英恵 神田拓 新谷智章 岡本哲治

Petrosiols A-E, neurotrophic diyne tetraols isolated from the Okinawan sponge Petrosia strongylata

• DOI: 10.1016/j.tet.2012.10.063
• 发表时间: 2013-01-07
• 期刊: TETRAHEDRON
• 影响因子: 2.1
• 作者: Horikawa, Kouhei;Yagyu, Takuya;Ojika, Makoto
• 通讯作者: Ojika, Makoto