I-Corps: Plasma-based Protein Footprinting

I-Corps：基于血浆的蛋白质足迹

• 批准号: 1801928
• 负责人: Benjamin Minkoff
• 金额: $ 5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-01-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1801928&HistoricalAwards=false
• 关键词: Corps; Plasma; based; Protein; Footprinting

The broader impact/commercial potential of this I-Corps will be to enable faster development of protein-based therapeutic drugs, such as antibodies and biosimilars. The core technology allows for faster determination of structural features of such protein therapeutic agents, which is important because structure is essential to both the function and efficiency of a protein therapeutic. The technology can be applied at many points within a therapeutic's development, from the initial discovery and screening phases to testing a developed therapeutic's stability under a wide array of conditions and environments for quality control purposes. Given the significant fraction of protein therapeutics, specifically, within the rapidly growing biopharmaceutical market, we believe our technology will both be of significant value to this industry and enable faster development of therapeutics for treating diseases in the future.This I-Corps project further develops a technology that determines structural features of proteins in a liquid environment. This is significant not only because a protein's structure dictates its function and by learning about the structure much information is gained about the protein itself, but also because proteins natively exist within cells in liquid environments. A plasma is used to create a reactive molecule from water surrounding the protein, and by measuring the areas on the protein that get labeled with this molecule, one can visualize relevant regions of the protein. When another element is added that changes the structure of the protein, or binds to and masks one side/face of the protein, this is reflected is changing levels or areas of modification by the reactive molecule. In this way, the technique can quickly map protein structural changes.

这个i-Corps的更广泛的影响/商业潜力将使以蛋白质为基础的治疗药物，如抗体和生物仿制药，得到更快的开发。核心技术允许更快地确定这种蛋白质治疗剂的结构特征，这一点很重要，因为结构对蛋白质治疗的功能和效率都是必不可少的。这项技术可以应用于治疗药物开发的许多阶段，从最初的发现和筛选阶段到测试开发的治疗药物在各种条件和环境下的稳定性，以达到质量控制的目的。鉴于蛋白质疗法的重要份额，特别是在快速增长的生物制药市场中，我们相信我们的技术将对该行业具有重要价值，并使未来治疗疾病的疗法能够更快地发展。这个i-Corps项目进一步开发了一种确定液体环境中蛋白质结构特征的技术。这一点意义重大，不仅是因为蛋白质的结构决定了它的功能，通过了解蛋白质的结构获得了关于蛋白质本身的许多信息，还因为蛋白质天然地存在于液体环境中的细胞内。血浆被用来从蛋白质周围的水中产生一个活性分子，通过测量蛋白质上标记该分子的区域，人们可以可视化蛋白质的相关区域。当加入另一种改变蛋白质结构的元素，或结合并掩盖蛋白质的一面/面时，这反映在反应分子的修饰水平或区域发生变化。通过这种方式，这项技术可以快速绘制出蛋白质结构的变化。