GOALI: Advanced biomanufacturing with inducible feedback promoters

使用微生物和生物质生产化学品是使用石油作为原材料的工艺的替代方案。在商业规模上实现这一点是困难的。该项目将开发一种新的工具来改进微生物化学生产。该工具将利用微生物感知环境的能力来提高性能。这些微生物将产生各种工业化学品。对社区的外展将采取多种形式。其中一个涉及在当地一所高中和小学为代表不足的人群提供服务的动手示威活动。更广泛的公众将通过芝加哥地区Maker Faire的摊位参与进来。这些活动旨在激发人们对生物学的兴趣，将其作为解决社会挑战的工具。他们还应该扩大从事STEM职业并进入生物制造工作的学生队伍。IFP(可诱导反馈启动子)代表了代谢途径控制和优化的新调控概念。它们将自然产生的压力反应反馈与纯粹的感应定时控制结合在一起。诱导性允许快速探索滴定和途径表达的时间。压力响应反馈允许自主表达优化。中心目标是在两个工业上重要的代谢途径的背景下开发和验证IFPS：萜类氧化和正丁醇生产。将通过验证IFP在每条途径中的监管方法的目标来实现这一目标。我们将创建一个适用于不同代谢途径的独特IFP的社区资源。与工业生物过程应用直接相关的IFP将通过群体感应和生物传感器使用自主定时控制进行微调。这些努力将与行业合作伙伴合作完成，他们将提供批判性的观点，使IFP能够为行业提供最大可能的价值。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

Producing chemicals using microbes and biomass is an alternative to processes that use petroleum as a raw material. Performing this at a commercial scale is difficult. This project will develop a new tool to improve microbial chemical production. The tool will use the microbe's ability to sense its environment to improve performance. The microbes will produce a variety of industrial chemicals. Outreach to the community will take many forms. One involves hands-on demonstrations in a local high school and elementary school serving underrepresented populations. The broader public will be engaged through booths at Chicago-area Maker Faires. These activities are designed to stimulate interest in biology as a tool to solve societal challenges. They should also expand the pool of students pursuing STEM careers and entering the biomanufacturing workforce.IFPs (inducible feedback promoters) represent a new regulatory concept for metabolic pathway control and optimization. They combine naturally occurring stress responsive feedback with purely inducible timing control. Inducibility allows titration and timing of pathway expression to be rapidly explored. Stress responsive feedback allows autonomous expression optimization. The central objective is to develop and validate IFPs in the context of two industrially important metabolic pathways: terpenoid oxygenation and n-butanol production. This objective will be pursued through aims that validate the approach of IFP regulation in each pathway. We will create a community resource of unique IFPs applicable to diverse metabolic pathways. IFPs that are directly relevant for industrial bioprocess application will be fine-tuned using autonomous timing control through quorum-sensing and biosensors. These efforts will be done in collaboration with industry partners who will provide critical perspective to allow development of IFPs that provide the greatest possible value to industry.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.