GOALI: Effect of Hydroxylamine on the Structure and Function of Nitrifying Biofilms

目标：羟胺对硝化生物膜结构和功能的影响

• 批准号: 1805406
• 负责人: Robert Nerenberg
• 金额: $ 33.01万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1805406&HistoricalAwards=false
• 关键词: GOALI; Effect; Hydroxylamine; Structure; Function

Wastewater treatment is needed for environmental protection, but most treatment processes are very energy intensive. A recently developed biological treatment process, called partial nitritation-anammox (PNA), can save energy. However, it is difficult to use in most treatment processes because it requires the suppression of some bacteria commonly found in wastewater. This research explores a new type of reactor configuration, along with the addition of the chemical hydroylamine, that may allow PNA to be effectively used for wastewater treatment. The research involves a collaboration with SUEZ, an industrial partner. It will train undergraduate and graduate students, as well as a post-doctoral researcher, and will include outreach to a community college with a new program in vertical farms.A collaborative study between the University of Notre Dame and SUEZ, a leader in membrane technologies, is proposed for a novel biofilm-based treatment technology that allows control of the internal physical and chemical environment in the biofilm. This method enables partial nitritation in existing wastewater treatment plants, a critical bottleneck in the energy-efficient PNA process. Furthermore, this biofilm environment control could enable other critical functions not currently possible in wastewater treatment, potentially serving as a disruptive technology. A key part of the new treatment process is the supply of chemicals to biofilms. In particular, this project will investigate how the supply of hydroxylamine, an intermediate of ammonia oxidizing bacteria (AOB) that can stimulate AOB growth and suppress nitrite-oxidizing bacteria (NOB) will alter the effectiveness of the PNA process. Researchers will first characterize the effects of hydroxylamine on the growth kinetics of AOB and NOB. Then the effects of hydroxylamine spikes on the structure and function of nitrifying biofilms will be explored using micro sensors, molecular tools, and imaging. An advanced biofilm model will be used to predict the best strategies for PNA. Finally, pilot-scale studies will be carried out to determine the process effectiveness in the field and to gather data and recommendations for scale-up and potential commercialization. This is the first research to study the effects of hydroxylamine on the microbial community structure of nitrifying biofilms. By determining the kinetics of AOB and NOB during and following exposure to hydroxylamine, this work will provide insights into fundamental inhibition mechanisms. This information will also provide a foundation for predicting the behavior of biofilms intermittently exposed to hydroxylamine. By studying mixed-culture biofilms with microsensors and molecular tools, the research could reveal potential niches for novel bacteria. The modeling and biofilm experiments will help engineer more effective water treatment processes. Workshops, lectures, and seminars at the University of Notre Dame and SUEZ will disseminate knowledge on biofilm research and process development to undergraduate, graduate, and Ivy Tech Community College students.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

废水处理是环境保护的需要，但大多数处理过程都是非常耗能的。 最近开发的生物处理工艺，称为部分硝化-厌氧氨氧化（PNA），可以节省能源。 然而，它很难用于大多数处理过程，因为它需要抑制废水中常见的一些细菌。 本研究探讨了一种新型的反应器配置，沿着添加化学羟基胺，这可能会使PNA有效地用于废水处理。 该研究涉及与工业合作伙伴苏伊士的合作。 它将培养本科生和研究生，以及博士后研究员，并将包括推广到一个社区大学的垂直农场的新计划。巴黎圣母院大学和苏伊士之间的合作研究，膜技术的领导者，提出了一种新的生物膜为基础的处理技术，允许控制生物膜中的内部物理和化学环境。这种方法能够在现有的废水处理厂中实现部分亚硝化，这是节能PNA工艺的关键瓶颈。此外，这种生物膜环境控制可以实现目前在废水处理中不可能实现的其他关键功能，可能成为一种破坏性技术。 新处理工艺的一个关键部分是向生物膜供应化学品。 特别是，该项目将研究羟胺的供应如何改变PNA工艺的有效性，羟胺是氨氧化细菌（AOB）的中间体，可以刺激AOB生长并抑制亚硝酸盐氧化细菌（NOB）。研究人员将首先表征羟胺对AOB和NOB生长动力学的影响。然后，羟胺尖峰的硝化生物膜的结构和功能的影响将探索使用微传感器，分子工具，和成像。 先进的生物膜模型将用于预测PNA的最佳策略。最后，将进行中试规模研究，以确定该领域的工艺有效性，并收集数据和建议，以扩大规模和潜在的商业化。本研究首次研究了羟胺对硝化生物膜微生物群落结构的影响。通过确定的动力学AOB和NOB期间和之后暴露于羟胺，这项工作将提供见解的基本抑制机制。这些信息也将为预测间歇暴露于羟胺的生物膜的行为提供基础。 通过使用微传感器和分子工具研究混合培养生物膜，该研究可以揭示新细菌的潜在生态位。模型和生物膜实验将有助于设计更有效的水处理工艺。在圣母大学和苏伊士举办的研讨会、讲座和研讨会将向本科生、研究生和常春藤理工社区学院的学生传播生物膜研究和工艺开发的知识。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。