Adhesion and Resuspension of Biological Particulate Matter in Early-Childhood Indoor Microenvironments

幼儿室内微环境中生物颗粒物的粘附和再悬浮

• 批准号: 1805804
• 负责人: Brandon Boor
• 金额: $ 31.55万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1805804&HistoricalAwards=false
• 关键词: Adhesion; Resuspension; Biological; Particulate; Matter

Infants and toddlers spend over 90% of their time indoors and are thus exposed to numerous agents in the indoor environment. House dust is heavily enriched with an incredible diversity of microbes and allergens. Early-life inhalation exposures to the microbial and allergenic content of indoor dust can play a significant role in both the development of, and protection against, asthma and allergic diseases. As infants crawl, play, and learn to walk, they stir-up (resuspend) concentrated clouds of biological particulate matter (bioPM: bacteria, fungi, pollen) from settled dust. The resuspension and adhesion of indoor bioPM remains poorly characterized. New research is needed to uncover the underlying transport mechanisms that govern bioPM exposures during this critical period in human immunological development. The goal of this project is to explicitly elucidate the fundamental mechanisms governing the adhesion, resuspension, and airborne transport of bioPM in early-childhood microenvironments. Exposure will be determined using a novel robot designed to simulate the movement of infants in the indoor environment and exposure to bioPM. This research has the potential to transform our understanding of infant exposure to bioPM at a critical stage in their development. Through a mechanistic framework, this project will make substantial contributions towards a fundamental understanding of the complex adhesion and human-driven resuspension processes that control the emissions, airborne transport, and fate of bioPM from settled dust in indoor environments. The specific objectives are: 1) investigate the adhesive interactions between bioPM and indoor surfaces through atomic force microscopy and explore the impact of bioPM morphology, hydrophobicity, charge, and humidity on adhesion forces; 2) develop a novel robotic platform to simulate infant/toddler movements informed by biometric data collected at the Purdue Miller Child Development Laboratory School (MCDLS) and to investigate aerodynamic, vibrational, and electrostatic bioPM removal forces; and 3) discover how infant/toddler movements resuspend bioPM through chamber experiments with the robot and develop a material balance model to predict bioPM fate and transport. This research will create new knowledge on how bioPM physiochemical properties affect adhesion forces to indoor surfaces. For the first time, bioPM removal mechanisms induced by forms of locomotion unique to infants will be determined. This project will generate extensive empirical data on bioPM adhesion forces and resuspension fractions. The novelty of the research is further exemplified by the integration of accelerometry data collected in a childcare center with infants/toddlers from 6 weeks to 3 years of age into the design of a robot for use in research at the interface of aerosol physics and microbiology. Lastly, the study will make innovative use of a Raspberry Pi-based low-cost particle sensor array to explore spatiotemporal variations in concentrations of resuspended bioPM which will infom the development of a multi-zone material balance model. Undergraduate students will participate in the bioPM experiments, in service learning projects on indoor air quality issues in K-12 schools as part of an interdisciplinary Engineering Projects in Community Service (EPICS) team, and in the development of the Raspberry Pi-based sensors and robot. New graduate course content on bioPM material balance modelling will be created. The project will actively involve Purdue MCDLS teachers, parents, custodial staff, and building facility managers to engage those who interact directly with infants and their indoor environments.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

婴幼儿90%以上的时间都在室内度过，因此会暴露于室内环境中的许多物质。室内灰尘中富含令人难以置信的微生物和过敏原多样性。 早期吸入暴露于室内灰尘的微生物和过敏原含量可以在哮喘和过敏性疾病的发展和预防方面发挥重要作用。 当婴儿爬行、玩耍和学习走路时，他们会从沉降的灰尘中搅动（重新悬浮）生物颗粒物（bioPM：细菌、真菌、花粉）的浓缩云。 室内生物颗粒物的再悬浮和粘附特性仍然很差。 需要进行新的研究，以揭示在人类免疫发育的这一关键时期，控制生物颗粒暴露的潜在运输机制。 这个项目的目标是明确阐明的基本机制，管理的粘附，再悬浮，和空气中的运输的bioPM在幼儿期微环境。将使用一种新型机器人来确定接触情况，该机器人旨在模拟婴儿在室内环境中的运动和生物颗粒物的接触情况。这项研究有可能改变我们对婴儿在其发育的关键阶段接触生物颗粒的理解。通过一个机械的框架，该项目将作出实质性的贡献，对复杂的粘附和人为驱动的再悬浮过程，控制排放，空气中的运输，并从室内环境中的沉降灰尘的生物颗粒物的命运的基本理解。 具体目标是：1）通过原子力显微镜研究生物颗粒与室内表面之间的粘附相互作用，并探索生物颗粒形态、疏水性、电荷和湿度对粘附力的影响; 2）开发一种新型的机器人平台，以模拟由普渡米勒儿童发展实验学校（MCDLS）收集的生物特征数据提供信息的婴儿/幼儿运动，并研究空气动力学，振动，和静电bioPM去除力;和3）发现婴儿/幼儿的运动如何重新悬浮bioPM通过室实验与机器人和开发一个材料平衡模型来预测bioPM的命运和运输。 这项研究将创造新的知识如何生物颗粒的理化性质影响粘附力的室内表面。将首次确定婴儿特有的运动形式引起的生物颗粒物去除机制。 该项目将产生大量关于生物颗粒粘附力和再悬浮分数的经验数据。 该研究的新奇进一步体现在将在儿童保育中心收集的6周到3岁的婴儿/幼儿的加速度数据整合到用于气溶胶物理学和微生物学接口研究的机器人设计中。最后，该研究将创新性地使用基于Raspberry Pi的低成本颗粒传感器阵列来探索再悬浮生物颗粒物浓度的时空变化，这将为多区域物质平衡模型的开发提供信息。 本科生将参加bioPM实验，在K-12学校的室内空气质量问题的服务学习项目，作为社区服务（EPICS）团队的跨学科工程项目的一部分，并在树莓派为基础的传感器和机器人的开发。将创建关于生物颗粒物质平衡建模的新的研究生课程内容。该项目将积极参与普渡MCDLS教师，家长，监护人员和建筑设施管理人员，以吸引那些直接与婴儿及其室内环境互动的人。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。

项目成果

Investigating How Occupancy and Ventilation Mode Influence the Dynamics of Indoor Air Pollutants in an Office Environment

研究占用和通风模式如何影响办公环境中室内空气污染物的动态

• 发表时间: 2020
• 期刊: Refrigerating & Air Conditioning Engineers (ASHRAE
• 作者: Jiang, J.;Wu, T.;Wagner, D.N.;Stevens, P.S.;Huber, H.;Tasoglou, A.;and Boor, B.E.
• 通讯作者: and Boor, B.E.

Real-time measurements of fluorescent aerosol particles in a living laboratory office under variable human occupancy and ventilation conditions

• DOI: 10.1016/j.buildenv.2021.108249
• 发表时间: 2021-11
• 期刊: Building and Environment
• 影响因子: 7.4
• 作者: S. Patra;Tianren Wu;Danielle N. Wagner;Jinglin Jiang;Brandon E. Boor

Influence of Mechanical Ventilation Systems and Human Occupancy on Time-Resolved Source Rates of Volatile Skin Oil Ozonolysis Products in a LEED-Certified Office Building

机械通风系统和人类居住对 LEED 认证办公楼中挥发性皮肤油臭氧分解产品时间分辨源速率的影响

• DOI: 10.1021/acs.est.1c03112
• 发表时间: 2021
• 期刊: Environmental Science & Technology
• 影响因子: 11.4
• 作者: Wu, Tianren;Tasoglou, Antonios;Huber, Heinz;Stevens, Philip S.;Boor, Brandon E.
• 通讯作者: Boor, Brandon E.

Spatial seated occupancy detection in offices with a chair-based temperature sensor array

• DOI: 10.1016/j.buildenv.2020.107360
• 发表时间: 2021
• 期刊: Building and Environment
• 影响因子: 7.4
• 作者: Danielle N. Wagner;Aayush Mathur;Brandon E. Boor

A machine learning field calibration method for improving the performance of low-cost particle sensors

• DOI: 10.1016/j.buildenv.2020.107457
• 发表时间: 2021-01-22
• 期刊: BUILDING AND ENVIRONMENT
• 影响因子: 7.4
• 作者: Patra, Satya S.;Ramsisaria, Rishabh;Boor, Brandon E.
• 通讯作者: Boor, Brandon E.