Inactivation of Legionella pneumophila harbored by amoebae using a nano-enabled alternative technology: Application and outreach to the Colonias in Texas
使用纳米替代技术灭活阿米巴虫所携带的嗜肺军团菌:在德克萨斯州殖民地的应用和推广
基本信息
- 批准号:1805958
- 负责人:
- 金额:$ 32.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-01 至 2022-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Legionnaires' disease is a deadly form of pneumonia caused by the bacterial pathogen Legionella pneumophila. Several outbreaks of this waterborne disease have occurred in the U.S. since 1976, and new cases have been growing at a high rate. Exposure occurs when contaminated water droplets are inhaled (e.g., during showering) or aspirated. L. pneumophila can be harbored by free-living single-celled organisms (amoebae), which can shield L. pneumophila from traditional disinfection like a 'Trojan horse', thereby increasing risk for the spread of Legionnaires' disease. This project aims to develop a low-cost disinfection technology that can harness low-energy microwave radiation to inactivate L. pneumophila in drinking water. The work includes a major outreach program with the Texas colonias, or self-built communities, which are home to 400,000 low-income people, many of whom lack access to high quality drinking water. Successful development of this technology has the potential to ensure safe drinking water for the colonias and the Nation. The goal of the proposed irradiation-based technology is to generate interfacial heat and reactive oxygen species (ROS) to inactivate L. pneumophila harbored by amoebae. Amoebae will be lysed via interfacial heating, and the released L. pneumophila will be inactivated by heat and ROS. The proposed research will deploy fundamental nanoscience to develop a point-of-use (POU) and community-scale water treatment technology. The specific technical objectives of the proposed research plan are to (i) synthesize, characterize, and test MW-harnessing nanomaterials, (ii) understand the mechanisms of microbial inactivation by this MW-based nanotechnology, and (iii) engineer two devices containing immobilized nanomaterials to effectively inactivate L. pneumophila harbored by amoebae. The research will capitalize on the scientific advantages of novel MW spectral conversion to provide safe drinking water to low-income residents of the Texas colonias and beyond. Outreach activities will occur in elementary schools and colonias located in three school districts (two in El Paso County and one in Hidalgo County, both of which have high concentrations of colonias). Hands-on educational activities related to the research will be conducted in the schools, and water sampling and household surveys will be conducted in the colonias to gather information on water quality, water use, water-related health issues, and socioeconomic conditions. The outreach is unique because the information it yields will be utilized in the device design, thereby enabling the development of a socially-embedded technology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
军团病是一种由嗜肺军团菌引起的致命肺炎。自1976年以来,这种水传播疾病在美国爆发了几次,新病例一直在以很高的速度增长。当被污染的水滴被吸入(例如,淋浴时)或吸入时,就会发生接触。嗜肺乳杆菌可以由自由生活的单细胞生物(变形虫)庇护,它可以像“特洛伊木马”一样保护嗜肺乳杆菌不受传统消毒的影响,从而增加了军团病传播的风险。本项目旨在开发一种低成本的消毒技术,利用低能量微波辐射灭活饮用水中的嗜肺乳杆菌。这项工作包括在德克萨斯殖民地(即自建社区)开展一项重要的外展项目,这些社区居住着40万低收入人口,其中许多人无法获得高质量的饮用水。这项技术的成功开发有可能确保殖民地和全国的安全饮用水。提出的基于辐照技术的目标是产生界面热量和活性氧(ROS)来灭活阿米巴窝藏的嗜肺乳杆菌。变形虫通过界面加热裂解,释放的嗜肺乳杆菌通过热和活性氧灭活。拟议的研究将利用基础纳米科学来开发一种使用点(POU)和社区规模的水处理技术。拟议研究计划的具体技术目标是:(1)合成、表征和测试利用毫米波的纳米材料,(2)了解利用这种基于毫米波的纳米技术灭活微生物的机制,以及(3)设计两种包含固定化纳米材料的装置,以有效灭活由变形虫窝藏的嗜肺乳杆菌。该研究将利用新型毫瓦光谱转换的科学优势,为德克萨斯殖民地及其他地区的低收入居民提供安全的饮用水。外联活动将在三个学区的小学和殖民地进行(两个在埃尔帕索县,一个在伊达尔戈县,这两个学区都有高度集中的殖民地)。将在学校开展与研究有关的实际教育活动,并将在各殖民地进行水取样和住户调查,以收集有关水质、用水、与水有关的健康问题和社会经济条件的信息。外展是独特的,因为它产生的信息将在设备设计中利用,从而使社会嵌入式技术的发展成为可能。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Compromised Water Quality in Colonias of Nueces County, TX: A Vicious Cycle
德克萨斯州纽埃西斯县科洛尼亚斯的水质受损:恶性循环
- DOI:
- 发表时间:2019
- 期刊:
- 影响因子:0
- 作者:Rowles III, L. S.;Hossain, A. H.;Lawler, D. F.;Kirisits, M. J.;Araiza, I.;Saleh, N. B.
- 通讯作者:Saleh, N. B.
Inactivation of Legionella Pneumophila-Harbored by Amoebae Using a Nano-Enabled Alternative Technology
使用纳米替代技术灭活阿米巴携带的嗜肺军团菌
- DOI:
- 发表时间:2019
- 期刊:
- 影响因子:0
- 作者:Saleh, N. B.;Kirisits, M. J.;Ayres, C.
- 通讯作者:Ayres, C.
Legionella pneumophila inactivation potency of silver nanoparticles and ionic silver and copper enhanced with microwave radiation
微波辐射增强银纳米颗粒和离子银和铜的嗜肺军团菌灭活效力
- DOI:
- 发表时间:2019
- 期刊:
- 影响因子:0
- 作者:Ayres, C.;Saleh, N. B.;Kirisits, M. J.;Lawler, D. F.
- 通讯作者:Lawler, D. F.
Seasonal contamination of well-water in flood-prone colonias and other unincorporated U.S. communities
易发洪水的殖民地和其他美国非建制社区的井水季节性污染
- DOI:10.1016/j.scitotenv.2020.140111
- 发表时间:2020
- 期刊:
- 影响因子:9.8
- 作者:Rowles III, Lewis Stetson;Hossain, Areeb I.;Ramirez, Isac;Durst, Noah J.;Ward, Peter M.;Kirisits, Mary Jo;Araiza, Isabel;Lawler, Desmond F.;Saleh, Navid B.
- 通讯作者:Saleh, Navid B.
A Structural Equation Model to Decipher Relationships among Water, Sanitation, and Health in Colonias -Type Unincorporated Communities
解读殖民地型非法人社区中水、卫生设施和健康之间关系的结构方程模型
- DOI:10.1021/acs.est.0c05355
- 发表时间:2020
- 期刊:
- 影响因子:11.4
- 作者:Rowles, Lewis Stetson;Whittaker, Tiffany;Ward, Peter M.;Araiza, Isabel;Kirisits, Mary Jo;Lawler, Desmond F.;Saleh, Navid B.
- 通讯作者:Saleh, Navid B.
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Navid Saleh其他文献
Navid Saleh的其他文献
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{{ truncateString('Navid Saleh', 18)}}的其他基金
NNA Track 1: Collaborative Research: A Purpose-Driven Merger of Western Science and Indigenous Knowledge of Water Quality in Alaskan Communities
NNA 轨道 1:合作研究:西方科学与阿拉斯加社区水质知识的有目的的融合
- 批准号:
2022670 - 财政年份:2020
- 资助金额:
$ 32.79万 - 项目类别:
Standard Grant
RAPID: Collaborative Research: Transforming passive protective face masks toward active capture and inactivation of coronavirus with nano-assisted surfactant modification
RAPID:合作研究:通过纳米辅助表面活性剂改性,将被动防护口罩转变为主动捕获和灭活冠状病毒
- 批准号:
2028521 - 财政年份:2020
- 资助金额:
$ 32.79万 - 项目类别:
Standard Grant
Collaborative Research: EAGER: Interaction of carbon-metal nanohybrids at environmental interfaces
合作研究:EAGER:碳-金属纳米杂化物在环境界面的相互作用
- 批准号:
1602273 - 财政年份:2016
- 资助金额:
$ 32.79万 - 项目类别:
Standard Grant
UNS: Role of dopant concentration and distribution in the environmental behavior of indium tin oxide nanoparticles
UNS:掺杂剂浓度和分布在氧化铟锡纳米粒子环境行为中的作用
- 批准号:
1511826 - 财政年份:2015
- 资助金额:
$ 32.79万 - 项目类别:
Standard Grant
Collaborative Research: Fate, Transport, and Organismal Uptake of Rod-Shaped Nanomaterials
合作研究:棒状纳米材料的命运、运输和生物摄取
- 批准号:
1440261 - 财政年份:2014
- 资助金额:
$ 32.79万 - 项目类别:
Standard Grant
NUE: Sustainable Nanotechnology Education for Undergraduate Engineering Students
NUE:工程本科生的可持续纳米技术教育
- 批准号:
1445960 - 财政年份:2014
- 资助金额:
$ 32.79万 - 项目类别:
Standard Grant
Collaborative Research: Fate, Transport, and Organismal Uptake of Rod-Shaped Nanomaterials
合作研究:棒状纳米材料的命运、运输和生物摄取
- 批准号:
1335926 - 财政年份:2013
- 资助金额:
$ 32.79万 - 项目类别:
Standard Grant
Influence of diameter and chirality of single-walled carbon nanotubes on their fate and effects in the aquatic environment
单壁碳纳米管的直径和手性对其在水生环境中的命运和影响的影响
- 批准号:
0933484 - 财政年份:2009
- 资助金额:
$ 32.79万 - 项目类别:
Continuing Grant
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集成移动元件如何操纵军团菌。
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