Collaborative Research: Precise and Dexterous Single-Particle Manipulation Using Non-uniform AC Magnetic Fields

合作研究：利用非均匀交流磁场进行精确灵巧的单粒子操纵

• 批准号: 1808307
• 负责人: Rui Qiao
• 金额: $ 21.44万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-06-15 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1808307&HistoricalAwards=false
• 关键词: Collaborative; Research; Precise; Dexterous; Single

High-precision manipulation of single micro-particles such as biological cells and colloids in the liquid environment is a critical process in applications such as single-cell analysis. A myriad of methods has been developed to achieve such manipulation. However, few of them can simultaneously meet all the requirements in practical applications, e.g., high precision, robustness, ability to move particles along arbitrary paths, low cost, and good biocompatibility. Recently the principal investigators discovered a new kind of particle manipulation method, i.e., using non-uniform alternating magnetic fields to actuate an anisotropic magnetic cluster and further applying the actuated cluster to manipulate nonmagnetic particles. Unlike other magnetic manipulation methods, this method requires only low-frequency, weak magnetic fields, and two orders of magnitude less power to achieve the same transitional speed, and the entire setup is extremely cost-effective. However, the fundamental mechanisms underlying this method are not clear and the parameters to precisely control the cluster motion are unknown. This project seeks to resolve this challenge and thus to create a precise, dexterous, low-cost, and biocompatible method for manipulating single particles. The project can potentially enable better single-cell analysis and make such analysis more accessible to research and educational communities, thereby creating great scientific and societal impact. The project includes education programs involving undergraduate students with diverse ethnical backgrounds and regional K-12 students. Discoveries from the project will be disseminated to technical as well as general audiences.The objective of this project is to understand, prefect, and apply the newly discovered magnetic particle actuation method through two specific aims: (1) to understand the actuation of single magnetic particles using non-uniform alternating magnetic fields; (2) to investigate nonmagnetic particle manipulation through the actuation of single magnetic particles. These aims will be achieved by integrating magnetic particle fabrication, experimental characterization of particulate dynamics in liquids, and multiphysics simulations. These interdisciplinary activities will benefit from the synergistic collaboration of the two research teams at University of Georgia and Virginia Tech, which have a fruitful history of collaboration and demonstrated expertise in material synthesis, instrumentation, and experimental and computational studies of particle transport in low-Reynolds number flows. The results from this project will provide both the theoretical basis and practical guidelines for the effective design of systems to manipulate single particles and cells. This project will also create new knowledge on the dynamics of magnetic particles in liquid environments and hydrodynamic actuation of particles in low-Reynolds number flows, thereby simultaneously contributing to the fields of magnetic actuation, fluid dynamics, particle assembly, and biotechnology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在液体环境中对生物细胞和胶体等单个微粒进行高精度操作是单细胞分析等应用中的关键过程。已经开发了无数的方法来实现这种操纵。然而，在实际应用中，很少有人能同时满足所有要求，例如，高精度、鲁棒性、沿沿着任意路径移动颗粒的能力、低成本和良好的生物相容性。最近，主要研究人员发现了一种新的粒子操纵方法，即，使用非均匀交变磁场来激励各向异性磁性团簇，并进一步应用所激励的团簇来操纵磁性粒子。与其他磁操纵方法不同，这种方法只需要低频、弱磁场和两个数量级的功率就可以实现相同的过渡速度，整个装置极具成本效益。然而，这种方法的基本机制尚不清楚，精确控制团簇运动的参数是未知的。该项目旨在解决这一挑战，从而创造一种精确，灵巧，低成本和生物相容性的方法来操纵单个粒子。该项目有可能实现更好的单细胞分析，并使研究和教育界更容易获得这种分析，从而产生巨大的科学和社会影响。该项目包括涉及不同种族背景的本科生和地区K-12学生的教育计划。本项目的目的是通过两个具体目标来理解、完善和应用新发现的磁性粒子驱动方法：（1）理解使用非均匀交变磁场的单个磁性粒子的驱动;（2）研究通过单个磁性粒子的驱动来操纵磁性粒子。这些目标将通过整合磁性粒子制造，液体中颗粒动力学的实验表征和多物理场模拟来实现。这些跨学科活动将受益于格鲁吉亚大学和弗吉尼亚理工大学两个研究小组的协同合作，这两个研究小组在材料合成、仪器仪表以及低雷诺数流动中颗粒输运的实验和计算研究方面具有富有成效的合作历史和专业知识。该项目的结果将为有效设计操纵单粒子和细胞的系统提供理论基础和实践指导。该项目还将创造有关液体环境中磁性颗粒动力学和低雷诺数流动中颗粒流体动力学驱动的新知识，从而同时有助于磁驱动，流体动力学，颗粒组装，该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查进行评估，被认为值得支持的搜索.

项目成果

Magnetic Actuation of Surface Walkers: The Effects of Confinement and Inertia

• DOI: 10.1021/acs.langmuir.9b03487
• 发表时间: 2020-06-30
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Fang, Wen-Zhen;Ham, Seokgyun;Tao, Wen-Quan
• 通讯作者: Tao, Wen-Quan

Manipulation of Single Cells Using a Ferromagnetic Nanorod Cluster Actuated by Weak AC Magnetic Fields

使用弱交流磁场驱动的铁磁纳米棒簇操纵单细胞

• DOI: 10.1002/adbi.201800246
• 发表时间: 2018
• 期刊: Advanced Biosystems
• 影响因子: 4.1
• 作者: Zhu, Lu;Huang, Weijie;Yang, Fengchang;Yin, Lei;Liang, Shenxuan;Zhao, Wujun;Mao, Leidong;Yu, Xiaozhong;Qiao, Rui;Zhao, Yiping
• 通讯作者: Zhao, Yiping