Effect of combination of nano structuring and polyelectrolyte multilayers on differentiation of stem cells

细胞外基质代表一种基质，它将纳米尺度上的地形和化学线索与控制细胞行为的特定机械特性结合在一起。该方案的目的是了解具有特定粘弹性的纳米结构衬底对小鼠间充质干细胞系C3H/10T1/2的黏附、生长和分化的影响。这里将使用纳米球光刻技术在平面模型衬底上生成指定距离和大小的金结构。随后采用逐层技术对金结构进行修饰，得到具有不同粘弹性能的聚电解质多层膜。由于由纳米结构尺寸调节的焦点粘连的大小和数量以及底物的机械性能影响细胞的行为，因此对细胞的粘连、生长和分化的影响是可以预期的，这需要用细胞和分子生物学的方法来测量。总体而言，该项目可以提供有关地形和机械表面特性对干细胞行为影响的知识，这些知识可以在未来用于组织工程和再生医学领域的新生物材料的开发。

The extracellular matrix represents a substratum, which combines topographical and chemical cues in the nanometer scale with specific mechanical properties that control the behavior of cells. The objective of the proposal is directed to understand the effect of nanostructured substrata with specific viscoelastic properties on adhesion, growth and differentiation of the mouse mesenchymal stem cell line C3H/10T1/2. Nanosphere lithography will be applied here to generate gold structures with defined distance and size on flat model substrata. The subsequent modification of gold structures with layer-by-layer technique shall be applied here to generate polyelectrolyte multilayers with different viscoelastic properties. Since size and number of focal adhesions, which are regulated by the dimensions of nanostructures, as well as mechanical properties of substrata influence the behavior of cells, effects on adhesion, growth and differentiation of cells are expected, which shall be measured with cell and molecular biology methods. Overall, the project can provide knowledge on the effect of topographical and mechanical surface properties on the behavior of stem cells, which can be used in future for the development of new biomaterials in the field of tissue engineering and regenerative medicine.