权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Effect of combination of nano structuring and polyelectrolyte multilayers on differentiation of stem cells

纳米结构与聚电解质多层组合对干细胞分化的影响

基本信息

批准号：
233863163
负责人：
Professor Dr. Thomas Groth
金额：
--
依托单位：
Arbeitsgruppe Biomedizinische Materialien
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/233863163?language=en
关键词：
Effect combination nano structuring polyelectrolyte

项目摘要

The extracellular matrix represents a substratum, which combines topographical and chemical cues in the nanometer scale with specific mechanical properties that control the behavior of cells. The objective of the proposal is directed to understand the effect of nanostructured substrata with specific viscoelastic properties on adhesion, growth and differentiation of the mouse mesenchymal stem cell line C3H/10T1/2. Nanosphere lithography will be applied here to generate gold structures with defined distance and size on flat model substrata. The subsequent modification of gold structures with layer-by-layer technique shall be applied here to generate polyelectrolyte multilayers with different viscoelastic properties. Since size and number of focal adhesions, which are regulated by the dimensions of nanostructures, as well as mechanical properties of substrata influence the behavior of cells, effects on adhesion, growth and differentiation of cells are expected, which shall be measured with cell and molecular biology methods. Overall, the project can provide knowledge on the effect of topographical and mechanical surface properties on the behavior of stem cells, which can be used in future for the development of new biomaterials in the field of tissue engineering and regenerative medicine.

细胞外基质代表了一种基质，它结合了纳米尺度的地形和化学线索以及控制细胞行为的特定机械特性。该提案的目的是了解具有特定粘弹性特性的纳米结构基质对小鼠间充质干细胞系 C3H/10T1/2 粘附、生长和分化的影响。此处将应用纳米球光刻在平面模型基底上生成具有确定距离和尺寸的金结构。随后采用逐层技术对金结构进行改性，以生成具有不同粘弹性特性的聚电解质多层膜。由于由纳米结构尺寸调节的粘着斑的大小和数量以及基质的机械性能影响细胞的行为，因此预计会对细胞的粘附、生长和分化产生影响，这应通过细胞和分子生物学方法进行测量。总体而言，该项目可以提供有关地形和机械表面特性对干细胞行为影响的知识，这些知识将来可用于组织工程和再生医学领域新型生物材料的开发。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Stimuli-Responsive Multilayers Based on Thiolated Polysaccharides That Affect Fibroblast Cell Adhesion.

DOI：
10.1021/acsami.7b19022
发表时间：
2018-02
期刊：
ACS applied materials & interfaces
影响因子：
9.5
作者：
Pegah Esmaeilzadeh;Alexander Köwitsch;A. Liedmann;M. Menzel;B. Fuhrmann;Georg Schmidt;J. Klehm;T. Groth
通讯作者：
Pegah Esmaeilzadeh;Alexander Köwitsch;A. Liedmann;M. Menzel;B. Fuhrmann;Georg Schmidt;J. Klehm;T. Groth

Introduction of Laser Interference Lithography to Make Nanopatterned Surfaces for Fundamental Studies on Stem Cell Response.

引入激光干涉光刻来制作纳米图案表面，用于干细胞反应的基础研究