Collaborative Research: Chemical and genetic mechanisms driving microbial interactions in a model microbiome

合作研究：驱动模型微生物组中微生物相互作用的化学和遗传机制

• 批准号: 1817887
• 负责人: Rachel Dutton
• 金额: $ 34.97万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1817887&HistoricalAwards=false
• 关键词: Collaborative; Research; Chemical; genetic; mechanisms

Microbes live in complex communities throughout the environment, but it remains unclear how specific microbes become members or maintain their role in these communities. This project will explore how bacteria and fungi use chemicals they produce to interact with one another and their environment. The bacteria and fungi in this project are all derived from the remarkably stable communities of microbes that inhabit cheese rinds that are consumed around the world. Currently, there is a poor understanding of how the chemicals bacteria and fungi produce on fermented foods stabilize or destabilize the roles of the members of their communities. In the short term, this project will identify key chemicals and processes that bacteria and fungi use to interact with one another on cheese rinds. In the long term, studying these microbes will give insight into the key processes bacteria and fungi use to operate within a community. The key processes used in this relatively simple system will be extended to other complex systems to explore how microbes use their own chemistry to affect other members in their communities. Multispecies microbial communities (microbiomes) are important drivers of global ecosystems, human and animal health, and food production, but our understanding of the molecular mechanisms that drive the formation of these communities remains a significant gap in knowledge. A contributing factor to this gap is the fact that few biological systems are available where in situ microbial communities can be deconstructed and experimentally recreated in the lab. Because of the challenges in manipulating microbiomes, very little is known about the genetic and chemical basis of species interactions. Bacteria and fungi are known to produce and dedicate a large part of their genetic material to the production of specialized metabolites. However, many of the pathways remain cryptic or have yet to be discovered because they have not been implicated in bioactivity screening. In this project innovative mass spectrometry techniques will be combined with microbial genetic and genomic techniques and a novel model community system to determine the molecular mechanisms of species interactions and how these interactions shape the formation and stability of microbial communities. This research will uncover conserved genetic pathways that underlie microbial interactions and their accompanying metabolites.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

微生物生活在整个环境中的复杂群落中，但目前尚不清楚特定的微生物如何成为这些群落的成员或保持其在这些群落中的作用。该项目将探索细菌和真菌如何使用它们产生的化学物质与彼此及其环境相互作用。这个项目中的细菌和真菌都来自于非常稳定的微生物群落，它们栖息在世界各地消费的奶酪皮上。目前，人们对细菌和真菌在发酵食品中产生的化学物质如何稳定或破坏其社区成员的作用还知之甚少。在短期内，该项目将确定细菌和真菌在奶酪皮上相互作用的关键化学物质和过程。从长远来看，研究这些微生物将有助于深入了解细菌和真菌在社区内运作的关键过程。这个相对简单的系统中使用的关键过程将扩展到其他复杂系统，以探索微生物如何利用自身的化学反应来影响其社区中的其他成员。多物种微生物群落（微生物组）是全球生态系统、人类和动物健康以及食品生产的重要驱动因素，但我们对驱动这些群落形成的分子机制的理解仍然是知识上的一个重大差距。造成这一差距的一个因素是，很少有生物系统可以在实验室中解构和实验性地重建原位微生物群落。由于操纵微生物组的挑战，对物种相互作用的遗传和化学基础知之甚少。已知细菌和真菌产生并将其大部分遗传物质用于产生专门的代谢物。然而，许多途径仍然是神秘的或尚未被发现，因为它们没有涉及生物活性筛选。在该项目中，创新的质谱技术将与微生物遗传和基因组技术以及一种新的模型社区系统相结合，以确定物种相互作用的分子机制以及这些相互作用如何塑造微生物群落的形成和稳定性。这项研究将揭示微生物相互作用及其伴随代谢物的保守遗传途径。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。