Evolution of the response to fluctuating oxygen availability in facultative anaerobes
兼性厌氧菌对氧气可用性波动的反应的演变
基本信息
- 批准号:1818303
- 负责人:
- 金额:$ 79.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-01 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This project will lead to a better understanding of how microbes respond to changes in oxygen availability. Some organisms can only tolerate very small fluctuations in oxygen availability. Others, including many bacteria and fungi are able to live with or without oxygen, and at all oxygen concentrations in between. These organisms accomplish this flexibility by shifting how they generate energy from an oxygen-dependent process called aerobic respiration to oxygen-independent processes like fermentation. This flexibility allows these organisms to live in a wide variety of different environments, but it comes at a cost. The overall goal of this project is to better understand the molecular and genetic basis of that cost. To date, this has been investigated in a very small number of organisms, leaving a major gap in our understanding of why some organisms are better equipped than others to adjust to fluctuating oxygen availability in natural environments. This project will fill that gap by comparing the molecular response of a much larger number of bacteria. Results from this project will have implications for understanding how oxygen availability impacts interaction of bacteria with plant and animal hosts. Project results will also have implications for biotechnology applications, like biofuel generation and other engineered bioprocesses which depend on manipulation of oxygen availability in industrial settings. Finally, this project will provide training and educational opportunities at the university, outreach activities to increase science literacy for K-12 students, and professional development opportunities for high school and middle school teachers.This project will focus on the evolutionary impact of fluctuating oxygen availability on facultative-anaerobic bacteria. The project builds on preliminary growth rate and transcriptome data from a small number of strains of enterobacteria. Aim 1 will measure fitness impacts of standing variation in natural populations under rigorously controlled oxygen availability conditions using large-scale phenotyping. Aim 2 will link genetic and phenotypic variation using association studies with both genome sequences and transcriptomes, and population genetic tests for genome-wide footprints of balancing selection. A subset of implicated loci will be validated using genome engineering. Aim 3 will test whether regular fluctuations in oxygen availability in experimentally evolved populations leads to persistence of conditionally advantageous and/or antagonistically deleterious variation. Results from this project are expected to significantly advance understanding of the significance of natural variation in the response to oxygen availability and the molecular underpinnings of fitness tradeoffs in heterogeneous environments.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该项目将有助于更好地了解微生物如何对氧气供应的变化做出反应。有些生物只能忍受氧气供应的非常小的波动。其他生物,包括许多细菌和真菌,能够在有或没有氧气的情况下生存,以及在两者之间的所有氧气浓度下生存。这些生物体通过将它们如何产生能量从称为有氧呼吸的氧依赖性过程转变为发酵等不依赖氧的过程来实现这种灵活性。这种灵活性使这些生物能够生活在各种不同的环境中,但这是有代价的。该项目的总体目标是更好地了解这种成本的分子和遗传基础。到目前为止,这已经在非常少数的生物体中进行了研究,在我们理解为什么一些生物体比其他生物体更好地适应自然环境中波动的氧气供应方面留下了重大空白。该项目将通过比较大量细菌的分子反应来填补这一空白。该项目的结果将对理解氧气供应如何影响细菌与植物和动物宿主的相互作用产生影响。项目结果还将对生物技术应用产生影响,如生物燃料生产和其他依赖于工业环境中氧气供应的工程生物过程。最后,该项目将提供在大学的培训和教育机会,推广活动,以提高K-12学生的科学素养,并为高中和中学教师的专业发展机会。该项目将重点关注波动的氧气供应对兼性厌氧菌的进化影响。该项目建立在来自少量肠杆菌菌株的初步生长率和转录组数据的基础上。目标1将测量健身的影响,在严格控制的氧气供应条件下,使用大规模的表型在自然种群的立位变化。目标2将使用基因组序列和转录组的关联研究以及平衡选择的全基因组足迹的群体遗传测试将遗传和表型变异联系起来。将使用基因组工程来验证涉及的基因座的子集。目标3将测试是否有规律的波动,在实验进化的人群中的氧可用性导致持续的条件有利和/或拮抗有害的变化。该项目的结果预计将大大推进理解的意义,自然变化的反应,氧气供应量和分子基础的健身权衡在异质environments.This奖项反映了NSF的法定使命,并已被认为是值得支持,通过评估使用基金会的智力价值和更广泛的影响审查标准。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Nicole Perna其他文献
Treatment of complex multi-sourced industrial wastewater — New opportunities for nanofiltration membranes
- DOI:
10.1016/j.cherd.2021.01.005 - 发表时间:
2021-04-01 - 期刊:
- 影响因子:
- 作者:
Abhishek Premachandra;Susan O’Brien;Nicole Perna;Jacob McGivern;Ryan LaRue;David R. Latulippe - 通讯作者:
David R. Latulippe
Nicole Perna的其他文献
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{{ truncateString('Nicole Perna', 18)}}的其他基金
ATOL: Assembling a taxonomically balanced genome-scale reconstruction of the evolutionary history of the Enterobacteriaceae
ATOL:对肠杆菌科进化史进行分类平衡的基因组规模重建
- 批准号:
0936214 - 财政年份:2009
- 资助金额:
$ 79.82万 - 项目类别:
Continuing Grant
US-France Joint Workshop: Genome Analysis of the Plant Pathogen, Erwinia Chrysanthemi 3937, Madison, Wisconsin, June 2004
美法联合研讨会:植物病原体基因组分析,Erwinia Chrysanthemi 3937,威斯康星州麦迪逊,2004 年 6 月
- 批准号:
0340700 - 财政年份:2004
- 资助金额:
$ 79.82万 - 项目类别:
Standard Grant
U.S.-France Joint Workshop: Genome Analysis of the Plant Pathogen, Erwinia Chrysanthemi 3937
美法联合研讨会:植物病原体菊欧文氏菌 3937 的基因组分析
- 批准号:
0129203 - 财政年份:2002
- 资助金额:
$ 79.82万 - 项目类别:
Standard Grant
NSF/Sloan Foundation Postdoctoral Research Fellowship in Molecular Evolution
NSF/斯隆基金会分子进化博士后研究奖学金
- 批准号:
9626042 - 财政年份:1996
- 资助金额:
$ 79.82万 - 项目类别:
Fellowship Award
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