Studies on the structures and mechanisms of algal photoreceptors

藻类光感受器的结构与机制研究

• 批准号: 235156569
• 负责人: Professor Dr. Lars-Oliver Essen
• 金额: --
• 依托单位: Arbeitsgruppe Strukturbiochemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/235156569?language=en
• 关键词: Studies; structures; mechanisms; algal; photoreceptors

Light-dependent responses play major roles not only in the morphogenesis of higher plants, but also in the regulation of cellular responses by green and red algae. Several cryptochromes are now demonstrated to mediate blue-light and, in the case of aCRY from Chlamydomonas reinhardtii, even red-light responses in green algae. An important prerequisite for understanding their in vivo photobiology is first the identification of cognate antenna cofactors in algal cryptochromes. Secondly, analysis of their redox-state dependent structures will then allow to correlate their photochemistry and signalling function with structural alterations and delineate interaction sites with putative downstream regulators. Aureochromes are a novel class of blue-light-dependent transcription factors, which are found only in several algae and differ by their inverted domain organisation from other LOV-regulated signalling proteins. We will analyse their lit state structures by X-ray crystallography when complexed to cognate DNA and compare them with their dark state structures. Here, the final goal is to obtain the molecular basis for intra-molecular signal transfer and transcription activation by aureochromes.

光依赖反应不仅在高等植物的形态建成中起重要作用，而且在绿色和红藻的细胞反应调节中也起重要作用。几种隐花色素现在被证明介导蓝光，并且在莱茵衣藻的aCRY的情况下，甚至介导绿色藻类中的红光反应。了解其在体内光生物学的一个重要先决条件是首先在藻类隐花色素同源天线辅因子的识别。其次，分析其氧化还原状态依赖的结构，然后将允许相关的光化学和信号功能与结构的改变和划定相互作用的网站与推定的下游监管机构。Aureochromes是一类新的蓝光依赖性转录因子，仅在几种藻类中发现，与其他LOV调节的信号蛋白不同的是它们的反向结构域组织。我们将通过X射线晶体学分析它们与同源DNA复合时的亮态结构，并将其与暗态结构进行比较。在这里，最终的目标是获得的分子内信号传递和转录激活的金色素的分子基础。