I-Corps: Chlorite and Chlorite Dismutase Mediated Continuous Bioprocessing Hygiene Control
I-Corps:亚氯酸盐和亚氯酸盐歧化酶介导的连续生物加工卫生控制
基本信息
- 批准号:1837886
- 负责人:
- 金额:$ 5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-07-01 至 2019-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The broader impact/commercial potential of this I-Corps project is to eliminate antibiotic use during biomanufacturing in the chemical and biotechnology industries, and to enable robust, contamination-free continuous processes that reduce upstream costs by 20-50%. Currently the biotechnology industry is growing at an astounding 7.5% per annum with a predicted value of $760B by 2025. However, industrial fermentation plant failure data collected over a 15-year period suggests that 1 in 10 process cultures are subject to microbial contamination and associated production failure. Furthermore, while continuous bioreactor processes significantly improve productivity and reduce unit operation costs, most biotechnology industries shy away from them because of the inherent sensitivity to contamination. Reduced bioreactor failure rate and operational cost is essential for the economic viability of bio-commodity chemicals such as biofuels, as production costs are a big impediment to large-scale manufacture; and their market demand depends primarily on their price competitiveness with petroleum-derived counterparts. Our technology can increase the productivity and profit margin of these companies. With our technology, large-scale bio-commodity chemicals production could become economically feasible. In addition to reduced operational costs, antibiotic-free production alleviates antibiotic misuse in industries to promote a positive environmental and public heath impact.This I-Corps project comprises a novel biocide/biocide-resistant system that could be used to treat and prevent microbial contamination in bioreactors. This technology allows bioprocessing engineers to eliminate the microbial contamination problem by addition of a biocide into the bioprocessing hosts' growth medium. The biocide prohibits the outgrowth of accidental contaminants while a biocide-resistant enzyme protects the bioprocessing host. The system also contains an on/off switch that allows easy removal of the biocide as needed. Preliminary data demonstrates that heterologous expression of the biocide-resistant enzyme in Escherichia coli and subsequent directed evolution resulted in a biocide-resistant Escherichia coli with a half maximal inhibitory concentration (IC50) approximately 16 times higher than the negative controls. We further demonstrate that this biocide in combination with the expression of biocide-resistant enzyme can be used to successfully treat a contaminated bioreactor.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该I-Corps项目的更广泛影响/商业潜力是在化学和生物技术行业的生物制造过程中消除抗生素的使用,并实现可靠的无污染连续工艺,将上游成本降低20- 50%。目前,生物技术产业正以惊人的7.5%的年增长率增长,预计到2025年将达到7600亿美元。 然而,在15年期间收集的工业发酵工厂故障数据表明,十分之一的工艺培养物受到微生物污染和相关的生产故障。此外,虽然连续生物反应器工艺显著提高了生产率并降低了单位操作成本,但大多数生物技术工业都回避它们,因为它们对污染物具有固有的敏感性。 降低生物反应器故障率和运营成本对于生物燃料等生物商品化学品的经济可行性至关重要,因为生产成本是大规模制造的一大障碍;其市场需求主要取决于其与石油衍生产品的价格竞争力。我们的技术可以提高这些公司的生产力和利润率。有了我们的技术,大规模的生物商品化学品生产在经济上是可行的。除了降低运营成本外,无抗生素生产还减少了工业中的抗生素滥用,从而促进了对环境和公众健康的积极影响。I-Corps项目包括一种新型杀菌剂/抗杀菌剂系统,可用于处理和预防生物反应器中的微生物污染。该技术允许生物处理工程师通过将杀生物剂添加到生物处理宿主的生长培养基中来消除微生物污染问题。杀生物剂阻止意外污染物的生长,而抗杀生物剂的酶保护生物处理宿主。该系统还包含一个开/关开关,可以根据需要轻松去除杀菌剂。初步数据表明,在大肠杆菌中异源表达的杀菌剂耐药酶和随后的定向进化导致杀菌剂耐药大肠杆菌的半数最大抑制浓度(IC 50)比阴性对照高约16倍。我们进一步证明,这种杀生物剂与抗杀生物剂酶的表达相结合,可以用来成功地处理污染的bioreactor.This奖项反映了NSF的法定使命,并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。
项目成果
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John Coates其他文献
Responding to the needs of youth who are homeless: Calling for politicized trauma-informed intervention
满足无家可归青年的需求:呼吁政治化的创伤知情干预
- DOI:
10.1016/j.childyouth.2011.09.008 - 发表时间:
2012 - 期刊:
- 影响因子:3.3
- 作者:
Suzanne McKenzie;John Coates;H. Mcleod - 通讯作者:
H. Mcleod
Iwasawa theory of quadratic twists of X 0(49)
- DOI:
10.1007/s10114-017-6414-x - 发表时间:
2017-02-06 - 期刊:
- 影响因子:0.900
- 作者:
Junhwa Choi;John Coates - 通讯作者:
John Coates
Classical Iwasawa theory and infinite descent on a family of abelian varieties
经典岩泽理论和阿贝尔变种家族的无限血统
- DOI:
10.1007/s00029-021-00644-3 - 发表时间:
2020-08 - 期刊:
- 影响因子:0
- 作者:
John Coates;Li Jianing;Li Yongxiong - 通讯作者:
Li Yongxiong
An ‘Ecospiritual’ Perspective: Finally, a Place for Indigenous Approaches
“生态精神”视角:最终,本土方法的一个地方
- DOI:
- 发表时间:
2005 - 期刊:
- 影响因子:0
- 作者:
John Coates;M. Gray;Tiani Hetherington - 通讯作者:
Tiani Hetherington
The Environmental Crisis
环境危机
- DOI:
10.1300/j059v16n01_03 - 发表时间:
2005 - 期刊:
- 影响因子:2.4
- 作者:
John Coates - 通讯作者:
John Coates
John Coates的其他文献
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