Career: Nanoparticle-Antibody Conjugates in Medical Imaging and Environmental Sensing

职业：医学成像和环境传感中的纳米颗粒抗体偶联物

• 批准号: 1841848
• 负责人: Nicole Steinmetz
• 金额: $ 20.1万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2020-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1841848&HistoricalAwards=false
• 关键词: Career; Nanoparticle; Antibody; Conjugates; Medical

Non-technical:This CAREER award funded by the Biomaterials program in the Division of Materials Research to Case Western Reserve University will pioneer novel antibody-nanoparticle conjugates. Antibodies and their conjugates find applications across scientific disciplines; the combination of antibodies with nanoparticles provides a means for multiplexed, miniaturized devices with enhanced detection capabilities. A biology-inspired nanofabrication technology will be implemented for the design of protein-based diagnostics for health and environment monitoring. This work promises to advance the scientific knowledge underlying nanoparticle design rules for improved disease and pollutant detection. With respect to future and potential biomedical applications, the proposed biomaterials hold great potential for detection and sub-typing of tumors, or combined with therapeutic molecules for drug- and gene-delivery. Furthermore, the proposed nanoparticle-antibody arrays could find applications as sensors to detect and/or purify various pesticides, steroids, and other pollutants. Advanced medical imaging and environment sensing devices is the key to maintain healthy living environments and societies. An important further benefit is the training and education of students in an interdisciplinary environment fostering team sciences, bridging bioengineering, synthetic biology, biomedicine, and environmental sensing. K-12 and public engagement will be promoted through the established 'The Nanoman' multimedia outreach program.Technical:This award is to synthesize and study novel immunosensors, and to educate the public and next-generation leaders in biomolecular research and industry. Nanoparticle-antibody conjugates will be produced from nucleoprotein assemblies with integrated antibody-binding domains formed by the components of the tobacco mosaic virus. The aspect ratio (length:width) of the soft matter immunoconjugates will be tightly controlled; therefore providing a unique platform to investigate nanoparticle aspect ratio as a design parameter for enhanced medical imaging and environmental sensing. The proposed nanoparticle-antibody conjugates hold great potential for applications in biomedicine, including cancer detection, sub-typing of tumors, as well as drug- and gene-delivery. Furthermore, the biology-programmed nanofabrication technique will be extended to produce spatial arrays of nanoparticle-antibody conjugates on surfaces for sensing of pollutants in water sources. Screening and detection of diseases as well as environment monitoring are critical to maintain healthy living environments and societies. As part of the outreach activities, the multimedia educational program 'The Nanoman' will serve as a platform to peak interest in STEM research through video and gaming. Graduate and undergraduate student training will focus on synthetic biology and biomolecular engineering with emphasis on collaborative and interdisciplinary research.

非技术性：该CAREER奖由凯斯西储大学材料研究部的生物材料项目资助，将开创新型抗体-纳米颗粒缀合物。抗体及其缀合物在各个科学学科中都有应用;抗体与纳米颗粒的组合为具有增强检测能力的多路复用、小型化设备提供了一种手段。生物启发的纳米纤维技术将用于设计用于健康和环境监测的基于蛋白质的诊断。这项工作有望推进纳米粒子设计规则的科学知识，以改善疾病和污染物检测。关于未来和潜在的生物医学应用，所提出的生物材料在肿瘤的检测和分型方面具有巨大的潜力，或者与治疗分子结合用于药物和基因递送。此外，所提出的纳米颗粒抗体阵列可以作为传感器应用于检测和/或纯化各种农药，类固醇和其他污染物。先进的医学成像和环境传感设备是维持健康生活环境和社会的关键。另一个重要的好处是在跨学科环境中培养学生的培训和教育，培养团队科学，桥接生物工程，合成生物学，生物医学和环境传感。K-12和公众参与将通过既定的“纳米人”多媒体推广计划来促进。技术：该奖项旨在合成和研究新型免疫传感器，并教育公众和生物分子研究和产业的下一代领导者。纳米颗粒-抗体缀合物将由具有由烟草花叶病毒的组分形成的整合的抗体结合结构域的核蛋白组装体产生。软物质免疫缀合物的纵横比（长度：宽度）将被严格控制;因此提供了一个独特的平台来研究纳米颗粒纵横比作为增强的医学成像和环境传感的设计参数。所提出的纳米颗粒-抗体缀合物在生物医学中具有巨大的应用潜力，包括癌症检测、肿瘤亚型以及药物和基因递送。此外，生物程序化纳米纤维技术将被扩展到在表面上产生纳米颗粒-抗体缀合物的空间阵列，用于检测水源中的污染物。疾病的筛查和检测以及环境监测对于维持健康的生活环境和社会至关重要。作为外展活动的一部分，多媒体教育节目“纳米人”将作为一个平台，通过视频和游戏来提高人们对STEM研究的兴趣。研究生和本科生培训将侧重于合成生物学和生物分子工程，重点是合作和跨学科研究。