SBIR Phase I: The DNA Barcode Scanner: Democratizing Genetic Information For Conservation

SBIR 第一阶段：DNA 条形码扫描仪：使遗传信息民主化以进行保护

• 批准号: 1843702
• 负责人: Paul Bunje
• 金额: $ 22.5万
• 依托单位: CONSERVATION X LABS, PBC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1843702&HistoricalAwards=false
• 关键词: SBIR; Phase; DNA; Barcode; Scanner

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase I project is to place the power of genetic identification in the hands of those who need it, at the front lines of conservation. The DNA Barcode Scanner will enable anyone, anywhere, to perform a low-cost, rapid, automated DNA test to validate the identity of a tissue sample, without specialized training, expensive lab-based equipment, reagents, or reliable power, where it is needed most - in the field. This technological advance has the potential to revolutionize species identification to prevent species extinction, to protect consumers and our food supply, and to combat threats to global biodiversity. The innovation's commercial potential includes improving the safety and sustainability of our supply chains, combatting costly environmental fraud, and detecting pests and pathogens that threaten our nation's economy, security, and health. The initial market opportunity is to address unmet demand from seafood suppliers, distributors, and retailers; government regulators and enforcement agents; and conservation and natural resources entities, for increased transparency and integrity of seafood supply chains by ending seafood mislabeling and fraud, which occurs in over 30% the $17.1 billion dollar U.S. seafood market and is worth up to $250,000 per illegal import.The proposed project will develop the customizable DNA collection, extraction, and amplification cartridge that enables a user to go from tissue sample to species identification in a single device. This project focuses on the scientific and technical challenge of automating sample preparation. The company's goal is to create an integrated device that will validate seafood products (our first market) from sample collection to DNA extraction, DNA amplification, and, ultimately, species identification. The goals of this project are to 1) determine a reliable and intuitive method for collecting DNA from a seafood product and create a prototype collection tool, 2) prototype an easy-to-use and highly flexible DNA extraction and sample preparation component to provide DNA suitable for analysis, and 3) fully integrate microfluidic processes and molecular chemistry (DNA amplification and analysis) with clear validation of multiple species. They will design and test several prototypes of the collection, extraction, and amplification cartridge and test these with users to identify the most robust and simplest method for delivering confident species identification. Their objective is to deliver a fully functional prototype that is intuitive for users and can perform a seafood species validation test in multiple field environments.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个小企业创新研究（SBIR）第一阶段项目的更广泛的影响/商业潜力是将遗传鉴定的力量交给那些需要它的人，在保护的第一线。DNA条形码扫描仪将使任何人，任何地方，执行低成本，快速，自动化的DNA测试，以验证组织样本的身份，而无需专门的培训，昂贵的实验室设备，试剂或可靠的电源，在最需要的地方-在现场。这一技术进步有可能彻底改变物种识别，以防止物种灭绝，保护消费者和我们的食物供应，并应对对全球生物多样性的威胁。这项创新的商业潜力包括提高我们供应链的安全性和可持续性，打击代价高昂的环境欺诈，以及检测威胁我们国家经济、安全和健康的害虫和病原体。最初的市场机会是解决海产品供应商、分销商和零售商、政府监管机构和执法机构未满足的需求;以及保护和自然资源实体，通过结束海鲜错误标签和欺诈来提高海鲜供应链的透明度和完整性，这些错误标签和欺诈发生在171亿美元的美国海鲜市场的30%以上，价值高达250美元，拟议的项目将开发可定制的DNA收集、提取和扩增盒，使用户能够在单个设备中从组织样本到物种鉴定。该项目侧重于自动化样品制备的科学和技术挑战。该公司的目标是创建一个集成设备，将验证海鲜产品（我们的第一个市场）从样本收集到DNA提取，DNA扩增，并最终，物种识别。该项目的目标是：1）确定一种可靠和直观的方法，从海鲜产品中收集DNA，并创建一个原型收集工具，2）原型易于使用和高度灵活的DNA提取和样品制备组件，以提供适合分析的DNA，以及3）完全集成微流控过程和分子化学（DNA扩增和分析），并明确验证多个物种。他们将设计和测试几种收集、提取和扩增盒的原型，并与用户一起测试，以确定最可靠、最简单的方法来提供可靠的物种鉴定。他们的目标是提供一个功能齐全的原型，对用户来说是直观的，可以在多个现场环境中进行海鲜物种验证测试。这个奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。