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CAREER: Neural mechanisms of flexible sensorimotor processing in C. elegans

职业：秀丽隐杆线虫灵活感觉运动处理的神经机制

基本信息

批准号：
1845137
负责人：
Andrew Leifer
金额：
$ 80万
依托单位：
Princeton University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1845137&HistoricalAwards=false
关键词：
CAREER Neural mechanisms flexible sensorimotor

项目摘要

The brain interprets the world around an organism to guide its actions and movements, but exactly how the brain does so remains a mystery. This CAREER award seeks to study how the brain of a small worm detects information about its environment and transforms that information into actions. The worm's small brain makes it possible to study the flow of information as it traverses through the brain with a level of detail and completeness that is not currently possible in other more complicated animals. The investigation uses new approaches that the principal investigator developed to record activity of the worm's brain as it crawls. The results of this study will provide new insights into how brains flexibly process information and generate actions. The work is integrated with a comprehensive education and outreach plan. In one component, hands-on workshops will be held for public policy students to learn about genetic engineering and machine learning, two cutting-edge technologies that enable the proposed research and also have the potential to transform society.The PI will investigate circuit-level mechanisms underlying sensorimotor processing in the nematode C. elegans. The animal flexibly interprets sensory signals to drive motor outputs dependent upon internal state. In some states an applied mechanosensory signal will propagate through the network to elicit a motor response, while in other states it will not. This project uses whole brain calcium imaging at cellular resolution in freely moving C. elegans to map out the functional paths by which the sensory signals propagate. Specifically, the work seeks to locate where in the network mechanosensory signals transition into locomotory signals, and to identify where state information impinges on this path using a whole-brain approach. C. elegans, with its compact nervous system of only 302 neurons, optical transparency, genetic tractability and mapped connectome is well-suited to carrying out the investigation. Education and outreach components of this research grant target students of public policy, undergraduate physics and engineering students especially from underrepresented groups, and undergraduate neuroscience students.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

大脑解释有机体周围的世界来指导它的行动和运动，但大脑到底是如何做到这一点的仍然是一个谜。这一职业奖旨在研究小蠕虫的大脑如何探测到与其环境有关的信息，并将这些信息转化为行动。蠕虫的小大脑使得研究信息流成为可能，因为它在大脑中穿行，具有目前在其他更复杂的动物中无法实现的细节和完整性。这项调查使用了首席研究员开发的新方法来记录蠕虫爬行时大脑的活动。这项研究的结果将为大脑如何灵活地处理信息和产生行动提供新的见解。这项工作与一项全面的教育和外联计划相结合。在其中一个组成部分中，将为公共政策专业的学生举办动手研讨会，让他们学习基因工程和机器学习，这两项尖端技术使拟议的研究成为可能，也有可能改变社会。PI将研究线虫感觉运动处理的电路层面机制。这种动物灵活地解释感觉信号，根据内部状态驱动运动输出。在某些状态下，施加的机械感觉信号将通过网络传播以引起运动反应，而在其他状态下则不会。该项目使用细胞分辨率的全脑钙成像在自由移动的线虫中绘制出感觉信号传播的功能路径。具体地说，这项工作试图定位网络中机械感觉信号转换为运动信号的位置，并使用全脑方法确定状态信息在这条路径上的影响位置。线虫的神经系统紧凑，只有302个神经元，具有光学透明性、遗传易感性和有图谱的连接体，非常适合进行这项研究。该研究基金的教育和推广部分面向公共政策专业的学生，特别是来自代表性不足的群体的本科生物理和工程学学生，以及神经科学本科生。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Decoding locomotion from population neural activity in moving C. elegans.

DOI：
10.7554/elife.66135
发表时间：
2021-07-29
期刊：
eLife
影响因子：
7.7
作者：
Hallinen KM;Dempsey R;Scholz M;Yu X;Linder A;Randi F;Sharma AK;Shaevitz JW;Leifer AM
通讯作者：
Leifer AM

A high-throughput method to deliver targeted optogenetic stimulation to moving C. elegans populations.