CAREER: Investigating environmental acidification and temperature as drivers of morphological alteration and physiological deficits in auditory systems of soniferous fishes

职业：研究环境酸化和温度作为发声鱼类听觉系统形态改变和生理缺陷的驱动因素

• 批准号: 1846004
• 负责人: Andrij Horodysky
• 金额: $ 70万
• 依托单位: Hampton University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1846004&HistoricalAwards=false
• 关键词: CAREER; Investigating; environmental; acidification; temperature

Since the Industrial Revolution, the ocean has become 30% more acidic due to its uptake of carbon dioxide, and coastal areas and freshwaters are frequently more acidic still due to inputs from natural and agricultural land and human activities in adjacent watersheds. The goal of this CAREER project is to examine the effects of temperature and carbon dioxide exposure on the structure and function of the auditory (hearing) systems of closely-related sound-producing fishes of the croaker/drum family Sciaenidae. These fishes are model organisms for the study of fish hearing and communication, most support valuable recreational fisheries in habitats ranging from fresh through salt water, and nearly all produce sounds to aid in reproduction and/or to raise alarm when disturbed or threatened. While auditory sense is of great importance to these sound producing fishes, little is known about the effects of changing environmental conditions on fish hearing and communication. This project's interdisciplinary approach will make significant impacts in this rapidly emerging field that will be of broad interest to both the ocean science and recreational fishing stakeholder communities. The award supports recruitment and professional development of postdoctoral, graduate, and undergraduate researchers from underrepresented groups in STEM disciplines. Student training includes mastery of quantitative reasoning, critical thinking, and communication skills regarding environmental impacts on aquatic ecosystems and sustainable stewardship of future ecosystems. This CAREER Award will expand the interdisciplinary neurosensory laboratory developed at Hampton University under the PI's earlier Research Initiation Award to examine how changing environmental conditions over the next century may affect the form and function of auditory systems of phylogenetically-related soniferous fishes that rely on auditory communication during aggregate spawning. This project will apply electrophysiological techniques and morphological analyses to assay the effects of increased temperature and CO2 concentrations representative of projected changes over the next century on auditory morphology and neurosensory function in representative freshwater, estuarine, and fully marine sciaenid fishes. Using related fishes from a common phylogeny but differing habitats and life histories, this project will examine the effects of chronic aqueous CO2 exposure (i.e. acidification) and increased temperature on the: (1) morphological development of sensory structures via digital microscopy and computerized tomography (CT), (2) neurosensory performance of auditory systems via Auditory Brainstem Response, and (3) manipulation of the GABA neurotransmitter to alter sensory deficits during CO2 exposure. This CAREER project will enhance research capacity and provide critical infrastructure that will support continued research by the PI, a postdoctoral associate, and both graduate and undergraduate students from underrepresented backgrounds in STEM beyond the award's lifetime.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

自工业革命以来，由于吸收二氧化碳，海洋的酸度增加了30%，沿海地区和淡水的酸度往往更高，这是由于自然和农业土地的输入以及邻近流域的人类活动。这个职业项目的目标是研究温度和二氧化碳暴露对大黄鱼/鼓科发声鱼类密切相关的听觉(听力)系统的结构和功能的影响。这些鱼类是研究鱼类听力和交流的模式生物，大多数在从淡水到咸水的生境中支持有价值的休闲渔业，几乎所有鱼类都发出声音以帮助繁殖和/或在受到干扰或威胁时发出警报。虽然听觉对这些发声鱼类非常重要，但人们对环境条件变化对鱼类听力和交流的影响知之甚少。该项目的跨学科方法将在这一迅速崛起的领域产生重大影响，海洋科学和休闲渔业利益攸关方都将广泛关注这一领域。该奖项支持从STEM学科中代表性不足的群体中招聘博士后、研究生和本科生研究人员并进行专业发展。学生培训包括掌握关于环境对水生生态系统的影响和对未来生态系统的可持续管理的定量推理、批判性思维和沟通技能。这项职业奖将扩大汉普顿大学在PI早期研究启动奖下开发的跨学科神经感觉实验室，以研究下个世纪环境条件的变化如何影响在聚集产卵期间依赖听觉交流的与系统发育相关的含声鱼类的听觉系统的形式和功能。该项目将应用电生理学技术和形态分析来分析代表下个世纪预测变化的温度和二氧化碳浓度升高对具有代表性的淡水、河口和全海洋海鞘鱼类的听觉形态和神经感觉功能的影响。本项目将利用来自共同系统发育但不同栖息地和生活史的相关鱼类，研究长期接触水二氧化碳(即酸化)和温度升高对以下方面的影响：(1)通过数字显微镜和计算机断层扫描(CT)的形态发育；(2)通过听觉脑干反应改变听觉系统的神经感觉表现；以及(3)操纵GABA神经递质以改变二氧化碳暴露期间的感觉缺陷。这一职业项目将增强研究能力，并提供关键的基础设施，以支持博士后助理PI以及STEM中代表性不足背景的研究生和本科生在奖项有效期后继续研究。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Physiological limits to inshore invasion of Indo-Pacific lionfish (Pterois spp.): insights from the functional characteristics of their visual system and hypoxia tolerance

• DOI: 10.1007/s10530-020-02241-5
• 发表时间: 2020-03-16
• 期刊: BIOLOGICAL INVASIONS
• 影响因子: 2.9
• 作者: Hasenei, Aaron;Kerstetter, David W.;Brill, Richard W.
• 通讯作者: Brill, Richard W.

Exploiting common senses: sensory ecology meets wildlife conservation and management.

• DOI: 10.1093/conphys/coab002
• 发表时间: 2021
• 期刊: Conservation physiology
• 影响因子: 2.7
• 作者: Elmer LK;Madliger CL;Blumstein DT;Elvidge CK;Fernández-Juricic E;Horodysky AZ;Johnson NS;McGuire LP;Swaisgood RR;Cooke SJ
• 通讯作者: Cooke SJ