Collaborative Research: Physiological and Genetic Correlates of Reproductive Success in High- versus Low-Quality Weddell seals

合作研究：高品质威德尔海豹与低品质威德尔海豹繁殖成功的生理和遗传相关性

• 批准号: 1853384
• 负责人: Brandon Briggs
• 金额: $ 60.82万
• 依托单位: University of Alaska Anchorage Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1853384&HistoricalAwards=false
• 关键词: Collaborative; Research; Physiological; Genetic; Correlates

Within any population, some individuals perform better than others. These individuals may survive longer or produce more offspring. Weddell seals in Erebus Bay, Antarctica, provide an unparalleled opportunity to investigate how an animal's physiology, behavior, and genetic make-up contribute to lifetime reproductive success because they have been the subject of a long-term population monitoring study and are easily accessible during their reproductive season. This project will distinguish key differences in energy allocation, reproductive timing, and dive capacities between female Weddell seals with a history of frequently producing pups ("high-quality" group), versus females that have produced pups only infrequently ("low-quality" group). For each group of females, physiology and behavior during the nursing period will be analyzed to assess whether investments influence their probability of reproducing the following year. Whole genomes will be compared between groups to identify underlying genes that govern reproductive success and population stability in a long-lived mammal. This collaborative project will provide research opportunities and training to several undergraduate and graduate students at the three participating institutions. Results will be broadly disseminated through presentations and peer-reviewed publications, and to students via an extensive public outreach collaboration with museum programming, curriculum-aligned science lessons, and pedagogy training.Within any wild animal population there is substantial heterogeneity in reproductive rates and animal fitness. Not all individuals contribute to the population equally; some are able to produce more offspring than others and thus are considered to be of higher quality. This study aims to distinguish which physiological mechanisms (energy dynamics, aerobic capacity, and fertility) and underlying genetic factors make some Weddell seal females particularly successful at producing pups year after year, while others produce far fewer pups than the population average. In this project, an Organismal Energetics approach will identify key differences between high- and low-quality females in how they balance current and future reproductive success by tracking lactation costs, midsummer foraging success and pregnancy rates, and overwinter foraging patterns and live births the next year. Repeated sampling of individuals' physiological status (body composition, endocrinology, ovulation and pregnancy timing), will be paired with a whole-genome sequencing study. The second component of this study uses a Genome to Phenome approach to better understand how genetic differences between high- and low-quality females directly correspond to functional differences in transcription, translation, and ultimately phenotype. This component will contribute to the functional analysis and annotation of the Weddell seal genome. In combination, this project will make strides towards distinguishing the roles that plastic (physiological, behavioral) and fixed (genetic) factors play in complex, multifaceted traits such as fitness in a long-lived wild mammal. The project partners with established programs to implement extensive educational and outreach activities that will ensure wide dissemination to educators, students, and the public. It will contribute to a marine mammal exhibit at the Pink Palace Museum, and a PolarTREC science educator will participate in field work in Antarctica. This award is co-funded by the GEO-OPP-Antarctic Organisms and Ecosystems Program, BIO-IOS-Physiological Mechanisms and Biomechanics Program, and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在任何群体中，有些人比其他人表现得更好。这些个体可以存活更长时间或产生更多的后代。南极洲埃里伯斯湾的威德尔海豹提供了一个无与伦比的机会来研究动物的生理，行为和遗传组成如何有助于终身生殖成功，因为它们一直是长期人口监测研究的对象，并且在繁殖季节很容易接近。该项目将区分能量分配，生殖时间，和潜水能力的女性威德尔海豹与频繁生产的幼崽（“高质量”组）的历史，与女性很少生产幼崽（“低质量”组）之间的关键差异。对于每组雌性，将分析哺乳期的生理和行为，以评估投资是否影响其下一年繁殖的可能性。整个基因组将在各组之间进行比较，以确定在长寿哺乳动物中控制繁殖成功和种群稳定的潜在基因。这一合作项目将为三个参与机构的几名本科生和研究生提供研究机会和培训。研究结果将通过演讲和同行评议的出版物广泛传播，并通过与博物馆规划，博物馆对齐的科学课程和教学培训的广泛公共宣传合作向学生传播。在任何野生动物种群中，生殖率和动物适应性都存在很大的异质性。并非所有个体对种群的贡献都是相等的;有些个体能够产生比其他个体更多的后代，因此被认为具有更高的质量。这项研究的目的是区分哪些生理机制（能量动力学，有氧能力和生育能力）和潜在的遗传因素使一些威德尔海豹女性特别成功地生产幼崽年复一年，而其他人生产的幼崽远远低于人口平均水平。在这个项目中，有机体能量学方法将通过跟踪哺乳成本，仲夏觅食成功率和怀孕率，以及越冬觅食模式和下一年的活产来确定高质量和低质量雌性之间的关键差异，以平衡当前和未来的生殖成功。对个体的生理状态（身体组成、内分泌、排卵和怀孕时间）进行重复采样，将与全基因组测序研究配对。本研究的第二部分使用基因组表型方法，以更好地了解高质量和低质量女性之间的遗传差异如何直接对应于转录，翻译和最终表型的功能差异。这将有助于威德尔海豹基因组的功能分析和注释。总而言之，该项目将在区分可塑性（生理、行为）和固定（遗传）因素在复杂、多方面的特征（例如长寿野生哺乳动物的适应性）中所发挥的作用方面取得重大进展。该项目与既定方案合作，实施广泛的教育和推广活动，确保向教育工作者、学生和公众广泛传播。它将为粉红宫博物馆的海洋哺乳动物展览作出贡献，一名PolarTREC科学教育工作者将参加南极洲的实地工作。 该奖项由GEO-IOS-Antarctic Organisms and Ecosystem Program、BIO-IOS-Physiological Mechanisms and Biomechanics Program以及既定的刺激竞争性研究计划（EPSCoR）共同资助。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。