The Roles of Heterogeneity, Mechanics, and the Environment in Biofilm Growth and Emergent Properties

异质性、力学和环境在生物膜生长和新兴特性中的作用

• 批准号: 1853602
• 负责人: Howard Stone
• 金额: $ 120万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1853602&HistoricalAwards=false
• 关键词: Roles; Heterogeneity; Mechanics; Environment; Biofilm

Biofilms are ubiquitous surface-attached bacterial communities that can cause major problems in industrial settings by fouling surfaces and by clogging pipes and filtration devices. However, biofilms can also be beneficial, for instance, in waste-water treatment and in microbiomes. The project will employ three-dimensional imaging, genetic techniques, mechanical measurements of biofilm properties, and biophysical theory and modeling to provide a unified, multi-scale characterization of biofilms that spans from the level of the cell to that of tightly organized collectives of thousands of cells. The results will define how properties that emerge in the "collective" determine the biofilm's material properties. The project will further provide opportunities for interdisciplinary training within an integrated research framework in which students and postdocs from biology, chemistry, engineering, and physics spend significant time in laboratories other than their own. In this research, the three integrated thrusts are: (1) to explore the origins and consequences of behavioral heterogeneity in growing biofilms, (2) to characterize biofilm mechanics from the micro-scale (a single cell or cluster of cells) to the macro-scale (the biofilm), and (3) to determine how physico/chemical and mechanical features of the environment influence microscopic and macroscopic biofilm properties. The focus is on the model quorum-sensing marine bacterium, Vibrio cholerae. The fundamental understanding gained through this research will enable development of biological and engineering strategies to promote or discourage formation of bacterial biofilms in relevant industrial and other applied settings, and, by connecting micro-scale and environmental features to macroscopic properties, may enable the discovery and design of new materials. Finally, the interdisciplinary work will lead to understanding of spatial and temporal gene expression programs in biofilms at the single-cell level and reveal how those programs, in conjunction with environmental factors, dictate the large-scale architectural and mechanical features of these multicellular systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生物膜是普遍存在的表面附着的细菌群落，其可通过污染表面和堵塞管道和过滤装置而在工业环境中引起重大问题。然而，生物膜也可以是有益的，例如，在废水处理和微生物组中。该项目将采用三维成像，遗传技术，生物膜特性的机械测量以及生物物理理论和建模，以提供从细胞水平到数千个细胞的紧密组织集体的生物膜的统一，多尺度表征。结果将定义在“集体”中出现的属性如何确定生物膜的材料属性。该项目还将在综合研究框架内提供跨学科培训的机会，在该框架内，来自生物学，化学，工程学和物理学的学生和博士后将在自己的实验室之外花费大量时间。在这项研究中，三个综合的推力是：（1）探索生长生物膜中行为异质性的起源和后果，（2）从微观尺度（单个细胞或细胞簇）到宏观尺度（生物膜）表征生物膜力学，（3）确定环境的物理/化学和机械特征如何影响微观和宏观生物膜特性。重点是模式群体感应海洋细菌，霍乱弧菌。通过这项研究获得的基本理解将使生物和工程策略的发展，以促进或阻止在相关的工业和其他应用环境中形成细菌生物膜，并通过将微观尺度和环境特征与宏观特性联系起来，可以发现和设计新材料。最后，跨学科的工作将导致在单细胞水平上理解生物膜中的空间和时间基因表达程序，并揭示这些程序如何与环境因素相结合，决定了大-该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的智力价值和更广泛的评估来支持。影响审查标准。

项目成果

Single-cell resolution imaging of bacterial biofilms

细菌生物膜的单细胞分辨率成像

• 发表时间: 2020
• 期刊: Physical biology
• 影响因子: 2
• 作者: Yan, J.;Stone, H.A.;Wingreen, N.S.;Bassler, B.L.
• 通讯作者: Bassler, B.L.

Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy

• DOI: 10.1126/science.abb8501
• 发表时间: 2020-07-03
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Qin, Boyang;Fei, Chenyi;Bassler, Bonnie L.
• 通讯作者: Bassler, Bonnie L.

Hierarchical transitions and fractal wrinkling drive bacterial pellicle morphogenesis.

• DOI: 10.1073/pnas.2023504118
• 发表时间: 2021-05-18
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Qin B;Fei C;Wang B;Stone HA;Wingreen NS;Bassler BL
• 通讯作者: Bassler BL

Diffusiophoretic Particle Penetration into Bacterial Biofilms.

• DOI: 10.1021/acsami.3c03190
• 发表时间: 2023-07-19
• 期刊: ACS APPLIED MATERIALS & INTERFACES
• 影响因子: 9.5
• 作者: Somasundar, Ambika;Qin, Boyang;Shim, Suin;Bassler, Bonnie L.;Stone, Howard A.
• 通讯作者: Stone, Howard A.

Nonuniform growth and surface friction determine bacterial biofilm morphology on soft substrates

• DOI: 10.1073/pnas.1919607117
• 发表时间: 2020-04-07
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Fei, Chenyi;Mao, Sheng;Kosmrlj, Andrej
• 通讯作者: Kosmrlj, Andrej