Transcriptional Regulation of the Cytokinin Signaling Network

细胞分裂素信号网络的转录调控

• 批准号: 1856248
• 负责人: George Schaller
• 金额: $ 87.5万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1856248&HistoricalAwards=false
• 关键词: Transcriptional; Regulation; Cytokinin; Signaling; Network

The plant hormone cytokinin regulates many agronomically important traits, including grain yield, biomass, shoot and root architecture, senescence, and disease resistance. How a hormone regulates such a diverse array of traits remains a central question in biology. The goal of this project is to uncover how cytokinins regulate plant growth and development, with a particular focus on differences in regulation that occur in the shoot and root. Investigators use interdisciplinary methods to experimentally quantify and mathematically model gene regulatory networks underlying cytokinin signaling. The understanding gained by their work will facilitate predictions for how perturbation of cytokinin function alters plant development. Outcomes of this work will ultimately enable precise manipulation of plants to optimize performance in various agricultural settings. Broader impacts of the project will provide interdisciplinary training opportunities for undergraduate students, graduate students and postdoctoral fellows. In addition, through local partnerships, programs will be developed that aim to encourage education and science awareness among elementary and middle school students through participatory activities.Cytokinin modulates plant growth and development in a potentially tissue specific manner, stimulating cell proliferation in the shoot but inhibiting it in the root. Focusing on transcriptional networks initiated by type-B ARR transcription factors, which act at the top of a transcriptional cascade to mediate the cytokinin response, investigators use an integrated set of experimental methods to dissect the mechanisms by which cytokinin regulation occurs. A dynamic Bayesian network approach will be used to mathematically infer gene regulatory networks that mediate the cytokinin response in roots and shoots and identify unique regulatory aspects that correlate with the tissue specific effects of cytokinin. Using genetic approaches the transcriptional networks will be perturbed to determine the role that various mechanisms play in mediating signaling output, with perturbations focused on key elements that regulate cytokinin signaling sensitivity and downstream transcription factors associated with the signaling network. RNA-Seq will be used to globally characterize gene expression and ChIP-Seq to identify targets of the transcription factors. By iteratively using network modeling to guide experimental investigations, which in turn further inform the model, this project will result in a systems level understanding of cytokinin signaling and the mechanism by which various transcriptional factors enable cytokinin to mediate development associated phenotypes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物激素细胞分裂素调节许多重要的农艺性状，包括谷物产量、生物量、茎和根结构、衰老和抗病性。激素如何调节如此多样化的特征仍然是生物学中的一个核心问题。该项目的目标是揭示细胞分裂素如何调节植物生长和发育，特别关注茎和根中发生的调节差异。研究人员使用跨学科的方法来实验量化和数学模型的基因调控网络的细胞分裂素信号。通过他们的工作获得的理解将有助于预测细胞分裂素功能的扰动如何改变植物发育。 这项工作的成果将最终实现对植物的精确操纵，以优化各种农业环境中的性能。该项目的更广泛影响将为本科生、研究生和博士后研究员提供跨学科培训机会。此外，还将通过地方合作伙伴关系，制定旨在通过参与性活动鼓励中小学生教育和科学意识的计划。细胞分裂素以潜在的组织特异性方式调节植物的生长和发育，刺激茎部的细胞增殖，但抑制根部的细胞增殖。关注由B型ARR转录因子启动的转录网络，该转录因子在转录级联的顶部起作用以介导细胞分裂素反应，研究人员使用一套完整的实验方法来剖析细胞分裂素调节发生的机制。一个动态贝叶斯网络的方法将被用来从数学上推断基因调控网络，介导的细胞分裂素反应在根和芽，并确定独特的监管方面，与组织特异性的细胞分裂素的影响。使用遗传方法的转录网络将被扰动，以确定各种机制在介导信号输出中发挥的作用，扰动集中在调节细胞分裂素信号敏感性的关键元件和与信号网络相关的下游转录因子。RNA-Seq将用于全面表征基因表达，ChIP-Seq将用于鉴定转录因子的靶标。通过反复使用网络建模来指导实验研究，进而进一步为模型提供信息，该项目将导致对细胞分裂素信号传导和各种转录因子使细胞分裂素介导发育相关表型的机制的系统水平的理解。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的评估来支持影响审查标准。

项目成果

EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity

• DOI: 10.1073/pnas.2013161117
• 发表时间: 2020-10-27
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Acheampong, Atiako Kwame;Shanks, Carly;Kieber, Joseph J.
• 通讯作者: Kieber, Joseph J.

Function of the pseudo phosphotransfer proteins has diverged between rice and Arabidopsis

水稻和拟南芥中假磷酸转移蛋白的功能存在差异

• DOI: 10.1111/tpj.15156
• 发表时间: 2021
• 期刊: The Plant Journal
• 作者: Vaughan‐Hirsch, John;Tallerday, Emily J.;Burr, Christian A.;Hodgens, Charlie;Boeshore, Samantha L.;Beaver, Kevin;Melling, Allison;Sari, Kartika;Kerr, Ian D.;Šimura, Jan
• 通讯作者: Šimura, Jan

Role of the Cytokinin-Activated Type-B Response Regulators in Hormone Crosstalk

• DOI: 10.3390/plants9020166
• 发表时间: 2020-01
• 期刊: Plants
• 作者: Y. Zubo;G. Schaller

Divide and Conquer: The Initiation and Proliferation of Meristems

• DOI: 10.1080/07352689.2021.1915228
• 发表时间: 2021-03
• 期刊: Critical Reviews in Plant Sciences
• 影响因子: 6.9
• 作者: Michael F Schwartz;R. Peters;Aitch Hunt;Abdul-Khaliq Abdul-Matin-Abdul-Khaliq-Abdul-Matin-1438680318;Lisa Van den Broeck;Rosangela Sozzani

Mutagenomics: A Rapid, High-Throughput Method to Identify Causative Mutations from a Genetic Screen

• DOI: 10.1104/pp.20.00609
• 发表时间: 2020-12-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Hodgens, Charles;Chang, Nicole;Kieber, Joseph J.
• 通讯作者: Kieber, Joseph J.