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Signal Integration of the Ethylene and Cytokinin Hormonal Pathways

乙烯和细胞分裂素激素途径的信号整合

基本信息

批准号：
1856513
负责人：
George Schaller
金额：
$ 82.05万
依托单位：
Dartmouth College
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-05-15 至 2024-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1856513&HistoricalAwards=false
关键词：
Signal Integration Ethylene Cytokinin Hormonal

项目摘要

Ethylene and cytokinins are plant hormones that regulate many agronomically important traits, including fruit ripening, grain yield, shoot and root architecture, senescence, disease resistance, and drought resistance. Although these two hormones often antagonize the actions of each other, ethylene generally being considered a hormone that promotes aging of plant tissues while cytokinin promotes greening, these same two hormones sometimes act in a cooperative manner. The goal of this project is to uncover how these two hormones interact in the regulation of plant growth and development. A better understanding of plant hormone signaling will enable optimization of crops to improve their performance in various agricultural settings. The research project will provide excellent training opportunities for graduate students and postdoctoral fellows. In addition, through a local partnership, the project will establish a summer program aimed at developing education and science awareness through participatory activities that integrate science with art.This research project presents an integrated set of experiments to elucidate mechanisms of transcriptional crosstalk between the ethylene and cytokinin signaling pathways, building toward a systems level understanding of this process. The focus of the research is on the EIN3 and type-B ARR families of transcription factors that regulate the primary transcriptional responses to ethylene and cytokinin, respectively. The first objective is to characterize the role of combinatorial interactions between EIN3 and the type-B ARR transcription factors in the regulation of gene expression, initial results supporting both cooperative and antagonistic interactions. The second objective of this project is to characterize the role that physical interactions between EIN3 and the type-A ARRs, a family of negative regulators whose expression is strongly induced by cytokinin, play in modulating the activity of EIN3 and ethylene signaling. The third objective is to characterize the role that ethylene plays in activating the type-B ARRs independently of their activation by the cytokinin signaling pathway, this serving as an alternative pathway by which to regulate a subset of the ethylene responses. These studies will incorporate global analyses of gene expression and target binding by the transcription factors, using RNA-Seq and ChIP-Seq approaches. Models for interaction derived from these studies will be tested by mechanistic, transcriptomic, and genetic approaches, and placed within the context of the roles of ethylene and cytokinin in seedling growth.This award was co-funded by the Physiological Mechanisms and Biomechanics Program in the Division of Integrative Organismal Systems and the Cellular Dynamics and Function Cluster in the Division of Molecular and Cellular Biosciences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

乙烯和细胞分裂素是调节许多农艺学上重要的性状的植物激素，包括果实成熟、谷物产量、枝和根结构、衰老、抗病性和抗旱性。虽然这两种激素经常相互拮抗，乙烯通常被认为是促进植物组织老化的激素，而细胞分裂素促进绿化，但这两种激素有时会以合作的方式起作用。该项目的目标是揭示这两种激素在调节植物生长和发育中的相互作用。更好地了解植物激素信号将使作物优化，以提高其在各种农业环境中的表现。该研究项目将为研究生和博士后研究员提供极好的培训机会。此外，通过当地的合作伙伴关系，该项目将建立一个暑期项目，旨在通过参与性活动，科学与艺术相结合，发展教育和科学意识。该研究项目提出了一套完整的实验，以阐明乙烯和细胞分裂素信号通路之间的转录串扰机制，建立对这一过程的系统水平的理解。研究的重点是EIN 3和B型ARR家族的转录因子，分别调节乙烯和细胞分裂素的初级转录反应。第一个目标是表征EIN 3和B型ARR转录因子在基因表达调控中的组合相互作用的作用，初步结果支持合作和拮抗相互作用。该项目的第二个目标是表征EIN 3和A型ARR之间的物理相互作用的作用，A型ARR是一个由细胞分裂素强烈诱导表达的负调节因子家族，在调节EIN 3和乙烯信号传导的活性中发挥作用。第三个目标是表征乙烯在激活B型ARR中所起的作用，其独立于细胞分裂素信号通路的激活，这作为一种替代途径来调节乙烯反应的一个子集。这些研究将采用RNA-Seq和ChIP-Seq方法对基因表达和转录因子的靶点结合进行全球分析。来自这些研究的相互作用模型将通过机制、转录组学和遗传方法进行测试，并将其置于乙烯和细胞分裂素在幼苗生长中的作用的背景下。该奖项是共同的，由综合有机系统部的生理机制和生物力学计划以及分子和细胞生物科学部的细胞动力学和功能群资助。该奖项反映了NSF的基金会的使命是履行其法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。