Detection of tumor cells with stem cell characteristics in oral leukoplakia

口腔白斑中具有干细胞特征的肿瘤细胞的检测

• 批准号: 237290448
• 负责人: Professor Dr. Emeka Nkenke
• 金额: --
• 依托单位: Universitätsklinikum für Mund-, Kiefer- und Gesichtschirurgie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/237290448?language=en
• 关键词: Detection; tumor; cells; stem; cell

So far it is matter of debate if the established conservative as well as surgical interventions for the treatment of oral leukoplakia (OLP) are effective in preventing the development of oral squamous cell carcinoma (OSCC). Therefore, it seems reasonable to check if new therapy options can be adopted.It is the hypothesis of the present grant application that tumor cells with stem cell characteristics can be detected in OLP which transform to OSCC. Moreover, it is assumed that these tumor cells with stem cell characteristics express cancer-testis antigens (e.g. MAGE-A). The latter could serve as targets for the elimination of the tumor cells with stem cell characteristics.Previously, the working group has shown that 1) approx. 85 % of OLP, which transform to OSCC, express MAGE-A. Normally, MAGE-A can only be detected in malignomas. 2) tumor cells with stem cell characteristics of OSCC express MAGE-A. The study aims at checking if tumor cells with stem cell characteristics are expressed in OLP that transform to OSCC. Samples of sound oral mucosa and samples of OLP that did not transform to OSCC in a 5-years period are used as controls.If tumor cells with stem cell characteristics could be detected in OLP MAGE-A, these findings could serve as the basis for the establishment of new treatment strategies like a vaccination therapy.

到目前为止，是否有争议的是，既定的保守性和手术干预措施，用于治疗口腔白细胞（OLP）可以有效防止口腔鳞状细胞癌（OSCC）的发展。因此，检查是否可以采用新的治疗方案似乎是合理的。这是当前授予应用的假设，即可以在OLP中检测到具有干细胞特征的肿瘤细胞，从而转化为OSCC。此外，假定这些具有干细胞特征的肿瘤细胞表达癌症抗原（例如Mage-A）。后者可以用作消除具有干细胞特征的肿瘤细胞的靶标。 85％的OLP转换为OSCC，表达法师A。通常，法师A仅在恶性肿瘤中检测到。 2）OSCC表达法师A的干细胞特征的肿瘤细胞。该研究旨在检查具有变干细胞特征的肿瘤细胞是否在转化为OSCC的OLP中表达。声音口服粘膜的样品和未转化为5年期间OSCC的OLP样品被用作对照。如果可以在OLP MAGE-A中检测到具有干细胞特征的肿瘤细胞，则这些发现可以作为建立诸如疫苗接种疗法之类的新治疗策略的基础。