Detection of tumor cells with stem cell characteristics in oral leukoplakia

口腔白斑中具有干细胞特征的肿瘤细胞的检测

• 批准号: 237290448
• 负责人: Professor Dr. Emeka Nkenke
• 金额: --
• 依托单位: Universitätsklinikum für Mund-, Kiefer- und Gesichtschirurgie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/237290448?language=en
• 关键词: Detection; tumor; cells; stem; cell

So far it is matter of debate if the established conservative as well as surgical interventions for the treatment of oral leukoplakia (OLP) are effective in preventing the development of oral squamous cell carcinoma (OSCC). Therefore, it seems reasonable to check if new therapy options can be adopted.It is the hypothesis of the present grant application that tumor cells with stem cell characteristics can be detected in OLP which transform to OSCC. Moreover, it is assumed that these tumor cells with stem cell characteristics express cancer-testis antigens (e.g. MAGE-A). The latter could serve as targets for the elimination of the tumor cells with stem cell characteristics.Previously, the working group has shown that 1) approx. 85 % of OLP, which transform to OSCC, express MAGE-A. Normally, MAGE-A can only be detected in malignomas. 2) tumor cells with stem cell characteristics of OSCC express MAGE-A. The study aims at checking if tumor cells with stem cell characteristics are expressed in OLP that transform to OSCC. Samples of sound oral mucosa and samples of OLP that did not transform to OSCC in a 5-years period are used as controls.If tumor cells with stem cell characteristics could be detected in OLP MAGE-A, these findings could serve as the basis for the establishment of new treatment strategies like a vaccination therapy.

到目前为止，它是一个有争议的问题，如果既定的保守以及手术治疗口腔白斑（OLP）是有效的，以防止口腔鳞状细胞癌（OSCC）的发展。因此，检查是否可以采用新的治疗选择似乎是合理的。本授权申请的假设是，可以在转化为OSCC的OLP中检测到具有干细胞特征的肿瘤细胞。此外，假设这些具有干细胞特征的肿瘤细胞表达癌-睾丸抗原（例如MAGE-A）。后者可以作为消除具有干细胞特征的肿瘤细胞的靶点。85%的OLP转化为OSCC，表达MAGE-A。通常，MAGE-A只能在乳腺癌中检测到。2)具有OSCC干细胞特征的肿瘤细胞表达MAGE-A。该研究旨在检查具有干细胞特征的肿瘤细胞是否在转化为OSCC的OLP中表达。以健康口腔粘膜和5年内未转化为OSCC的OLP为对照，如果在OLP MAGE-A中检测到具有干细胞特征的肿瘤细胞，这些发现可以作为建立新的治疗策略（如疫苗治疗）的基础。