Fluorescent Probes for the Detection of Ethylene

用于检测乙烯的荧光探针

• 批准号: 1900482
• 负责人: Brian Michel
• 金额: $ 30万
• 依托单位: University of Denver
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1900482&HistoricalAwards=false
• 关键词: Fluorescent; Probes; Detection; Ethylene

Ethylene is an important chemicals in modern society, with impacts ranging from the production of plastics and other chemicals to playing a significant role in the food supply chain as the primary ripening hormone of some fruit. As a result, there has been a significant effort to detect this small molecule via instrumentation and with molecular recognition. While these efforts have led to some sensitive approaches for ethylene detection, methods for detecting ethylene in complex environments (such as inside a plant cell) do not currently exist. In this project, Professor Michel of the University of Denver is developing new small molecule probes that increase fluorescence upon reaction with ethylene. An understanding of the relationship between the structure of the probe and its signaling property in a specific environment are critical to the sensitive detection of ethylene. Fluorescent and color-producing probes have the unique ability to produce color within otherwise invisible chemical processes. This research is being developed into an integrated lecture-lab module for undergraduate organic students to provide context to underrepresented topics. Professor Michel develops outreach demonstrations at local high schools. Due to the desire to understand and control ethylene in the fruit supply chain, this research may have a broader impact on society by increasing the understanding of the molecular roles of ethylene in the ripening processes; thereby preventing premature food spoilage and reducing agricultural waste. Further, the graduate and undergraduate students working on this project are underrepresented minorities who are gaining a unique skill set due to the interdisciplinary nature of this project. With funding from the Chemical Structure, Dynamics, and Mechanisms B program of the Chemistry Division, Professor Michel of the University of Denver is developing new small molecule fluorescent probes for the detection of ethylene. Due to the relatively small and unreactive nature of ethylene under the confined conditions of living systems, an organometallic-based approach is being developed, which requires a fundamental understanding of the relationship between the ligands bound to the metal center and stoichiometric reactivity with ethylene. This research determines the optimal probes for a particular application, such as the detection of ethylene in relevant biological systems including A. thaliana and E. coli. Detection of ethylene in these organisms is being investigated with probe modifications tailored to the specific goals and challenges presented by these systems. The detection of gaseous ethylene in the atmosphere including from ripening fruit is also being investigated.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

乙烯是现代社会中的一种重要化学品，其影响范围从塑料和其他化学品的生产到作为某些水果的主要成熟激素在食品供应链中发挥重要作用。因此，通过仪器和分子识别来检测这种小分子已经有了重大的努力。虽然这些努力已经导致了一些敏感的乙烯检测方法，但目前还不存在在复杂环境中(如植物细胞内)检测乙烯的方法。在这个项目中，丹佛大学的米歇尔教授正在开发新的小分子探测器，这种探测器可以在与乙烯反应时增强荧光。了解探针的结构与其在特定环境中的信号特性之间的关系对于灵敏地检测乙烯是至关重要的。荧光和产生颜色的探针具有在其他看不见的化学过程中产生颜色的独特能力。这项研究正在发展成为本科生有机专业学生的综合讲课-实验模块，以提供未被充分代表的主题的背景。米歇尔教授在当地高中开展外展示范活动。由于希望了解和控制水果供应链中的乙烯，这项研究可能会通过增加对乙烯在成熟过程中的分子作用的了解，对社会产生更广泛的影响，从而防止食品过早变质，减少农业浪费。此外，参与这个项目的研究生和本科生是少数族裔，由于这个项目的跨学科性质，他们正在获得一套独特的技能。在化学系化学结构、动力学和机理B项目的资助下，丹佛大学的米歇尔教授正在开发用于检测乙烯的新型小分子荧光探针。由于乙烯在生命系统的受限条件下相对较小且不具活性，因此正在开发一种基于有机金属的方法，这需要从根本上了解结合在金属中心的配体与乙烯的化学计量反应性之间的关系。这项研究确定了特定应用的最佳探针，例如检测相关生物系统中的乙烯，包括A.thaliana和E.Coli。正在对这些生物体中乙烯的检测进行调查，根据这些系统提出的具体目标和挑战进行探针修改。对大气中包括成熟水果中的气态乙烯的检测也在调查中。这一裁决反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Steric modulation of CAACs controls orientation and ethenolysis performance

CAAC 的空间调节控制方向和乙烯醇分解性能

• DOI: 10.1016/j.checat.2023.100764
• 发表时间: 2023
• 期刊: Chem Catalysis
• 作者: Jensen, Katrina H.;Michel, Brian W.
• 通讯作者: Michel, Brian W.

Ligand-Directed Approach to Activity-Based Sensing: Developing Palladacycle Fluorescent Probes That Enable Endogenous Carbon Monoxide Detection

• DOI: 10.1021/jacs.0c06405
• 发表时间: 2020-09-16
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Morstein, Johannes;Hofler, Denis;Chang, Christopher J.
• 通讯作者: Chang, Christopher J.