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DEVELOPING AND INTEGRATING "-OMIC" TOOLS TO ELUCIDATE NANOPARTICLE TRANSPORT MECHANISM AND RESPONSES IN AGRICULTURAL CROPS

开发和集成“-Omic”工具来阐明纳米颗粒传输机制和农作物的反应

基本信息

批准号：
1901515
负责人：
Arturo Keller
金额：
$ 39万
依托单位：
University of California-Santa Barbara
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-05-15 至 2022-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1901515&HistoricalAwards=false
关键词：
DEVELOPING INTEGRATING OMIC TOOLS ELUCIDATE

项目摘要

Nanotechnology has gained considerable attention in agricultural applications, due to the unique properties of materials at the nanoscale. Nanomaterials also exhibit an ability to bypass biological barriers. These novel materials may elicit beneficial as well as undesirable responses depending on their composition, method of application, dose or the sensitivity of the crop species. There is limited understanding of how plants respond to these nanomaterials, how they take them up, and at what point the application results in undesirable effects. The project utilizes sensitive bio-analytical tools to study the response of plants to different doses of nanomaterials. The knowledge obtained from this study should serve to design safer nano-scale agrochemicals, improve their effectiveness and promote agricultural sustainability by reducing the use of active ingredients and undesirable accumulation of agrochemicals in soil and associated water bodies. The overarching goal of this research is to apply advanced analytical methods to understand potential challenges in agriculture that can improve crop productivity and health, while minimizing burden on overall environmental health. The proposed project will employ sensitive molecular "nomics" approaches to elucidate the mechanisms of uptake of metal oxide nanoparticles by crop plants and plant response at different doses. Multi-omic tools considered include high-throughput proteomics and metabolomics using advanced liquid chromatography-mass spectrometry. These tools will be used to probe the interactions at the protein and metabolite levels in plants exposed to metal oxide nanoparticles such as copper hydroxide, molybdenum oxide, manganese oxide and cerium oxide via foliar and soil application. Plasma-membrane proteins and apoplastic proteins are critical candidates for this study as they participate in communication between cells and the extracellular environment, ion transport, protein translocation/integration, and signal transduction. These protein in roots and leaves may be selectively enriched in the sample pool, helping to elucidate the main transport pathways. Analysis of hydrophilic and hydrophobic metabolites will also be fractionated for untargeted metabolomics. The candidate proteins and metabolites from untargeted -omic approach will be validated and quantified using targeted approaches across different exposure periods and concentrations, followed by meta-data integration to identify universal biomarkers of metal oxide nanoparticle exposure. The findings will provide a more comprehensive understanding of the routes of entry and localization of nanoparticles and simultaneous regulation of essential nutrients and plant metabolites. The results obtained will provide a benchmark for future research on targeted delivery of nutrients or other agrochemicals.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

由于纳米材料的独特性质，纳米技术在农业应用中获得了相当大的关注。纳米材料还表现出绕过生物屏障的能力。这些新材料可引起有益的以及不期望的反应，这取决于它们的组成、施用方法、剂量或作物物种的敏感性。对于植物如何对这些纳米材料做出反应，它们如何吸收它们，以及在什么情况下应用会导致不良影响，人们的理解有限。该项目利用敏感的生物分析工具研究植物对不同剂量纳米材料的反应。从这项研究中获得的知识应有助于设计更安全的纳米级农用化学品，提高其有效性，并通过减少活性成分的使用和农用化学品在土壤和相关水体中的不良积累来促进农业可持续性。这项研究的首要目标是应用先进的分析方法来了解农业中的潜在挑战，可以提高作物生产力和健康，同时尽量减少对整体环境健康的负担。拟议的项目将采用敏感的分子“组学”方法来阐明作物吸收金属氧化物纳米颗粒的机制以及植物对不同剂量的反应。考虑的多组学工具包括高通量蛋白质组学和代谢组学，使用先进的液相色谱-质谱。这些工具将用于探测通过叶面和土壤施用暴露于金属氧化物纳米颗粒（如氢氧化铜、氧化钼、氧化锰和氧化铈）的植物中蛋白质和代谢物水平的相互作用。质膜蛋白和质外体蛋白是本研究的关键候选者，因为它们参与细胞与细胞外环境之间的通信、离子转运、蛋白质易位/整合和信号转导。根和叶中的这些蛋白质可能选择性地富集在样品池中，有助于阐明主要的运输途径。亲水性和疏水性代谢物的分析也将被分级用于非靶向代谢组学。来自非靶向组学方法的候选蛋白质和代谢物将使用靶向方法在不同的暴露时间和浓度下进行验证和定量，然后进行元数据整合以识别金属氧化物纳米颗粒暴露的通用生物标志物。这些发现将为纳米颗粒的进入和定位途径以及必需营养素和植物代谢产物的同时调节提供更全面的了解。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。