Bacterial Reaction Centers With New Photochemical Properties

具有新光化学特性的细菌反应中心

• 批准号: 1904860
• 负责人: James Allen
• 金额: $ 64.2万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1904860&HistoricalAwards=false
• 关键词: Bacterial; Reaction; Centers; New; Photochemical

Deciphering the mechanisms by which the movement of protons is linked to electron transfer is an important challenge in understanding biochemical catalysis. Professors James Allen and JoAnn Williams of Arizona State University are directing their efforts to elucidate this linkage for chemical reactions that occur in photosynthesis, nature's fundamental process for converting light into chemical energy. Because control of coupling between proton and electron transfer is critical for achieving efficient energy conversion and chemical transformations, this project aims to generate a more complete understanding of the physical principles that govern these processes in complex systems. The activities offer societal benefits through broadening participation in science and engagement of the wider community. To overcome traditional low graduation rates and limited participation in science degree programs, this project provides students from a local community college with a personal research experience in a supportive environment that encourages their continuation in science. In another avenue of engagement, students work with artists to express the scientific concepts behind research in bioenergy and photosynthesis and present their integration of science and art to the local community.With this award, the Chemistry of Life Processes Program, with partial co-funding from the Molecular Biophysics Cluster in the Division of Molecular and Cellular Biosciences, is supporting Professors James Allen and JoAnn Williams of Arizona State University to help establish molecular concepts that explain how proteins effectively perform proton-coupled, multi-electron-transfer reactions. They are using novel protein design strategies to modify the bacterial reaction center, an integral membrane protein serving as the site of the primary photochemistry in photosynthesis. The yields of proton and electron transfer are being measured for different cofactors and protein environments. The experimental methods combine structure-based protein design, enabled by molecular biology and biochemical techniques, with analysis of the properties of the resulting complexes by optical and magnetic resonance spectroscopy. The project is designed to expand the tools available for designing proton-coupled, electron-transfer reactions for catalysis in biological systems and establish a framework applicable for understanding the progression of oxidation states in the fundamental water oxidation reactions of photosystem II.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

破解质子运动与电子转移的联系机制是理解生物化学催化的一个重要挑战。亚利桑那州立大学的詹姆斯艾伦和乔安威廉姆斯教授正致力于阐明光合作用中发生的化学反应的这种联系，光合作用是自然界将光转化为化学能的基本过程。由于控制质子和电子转移之间的耦合对于实现有效的能量转换和化学转化至关重要，因此该项目旨在更全面地了解在复杂系统中管理这些过程的物理原理。这些活动通过扩大对科学的参与和更广泛的社区参与来提供社会效益。为了克服传统的低毕业率和科学学位课程的参与有限，该项目为当地社区学院的学生提供了一个支持性的环境，鼓励他们继续从事科学研究的个人研究经验。在另一种参与方式中，学生与艺术家合作，表达生物能源和光合作用研究背后的科学概念，并向当地社区展示科学与艺术的融合。有了这个奖项，生命过程化学计划，部分共同资助来自分子和细胞生物科学部门的分子生物物理学集群，正在支持亚利桑那州立大学的詹姆斯艾伦和乔安威廉姆斯教授帮助建立分子概念，解释蛋白质如何有效地进行质子耦合，多电子转移反应。他们正在使用新的蛋白质设计策略来修改细菌反应中心，这是一种完整的膜蛋白，作为光合作用中主要光化学的位点。质子和电子转移的产量正在测量不同的辅因子和蛋白质环境。实验方法结合联合收割机基于结构的蛋白质设计，使分子生物学和生物化学技术，通过光学和磁共振光谱分析所得复合物的性质。该项目旨在扩展可用于设计质子耦合，表示"电子"之义该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查进行评估来支持的搜索.

项目成果

Influence of Hydrogen Bonds on the Electron-Phonon Coupling Strength/Marker Mode Structure and Charge Separation Rates in Reaction Centers from Rhodobacter sphaeroides.

• DOI: 10.1021/acs.jpcb.9b08388
• 发表时间: 2019-09
• 期刊: The journal of physical chemistry. B
• 作者: A. Khmelnitskiy;Joann C. Williams;James P. Allen;R. Jankowiak

A bound iron porphyrin is redox active in hybrid bacterial reaction centers modified to possess a four-helix bundle domain

结合的铁卟啉在修饰为具有四螺旋束结构域的杂合细菌反应中心中具有氧化还原活性

• DOI: 10.1007/s43630-021-00142-7
• 发表时间: 2022
• 期刊: Photochemical & Photobiological Sciences
• 影响因子: 3.1
• 作者: Allen, J. P.;Chamberlain, K. D.;Olson, T. L.;Williams, J. C.
• 通讯作者: Williams, J. C.

Bound manganese oxides capable of reducing the bacteriochlorophyll dimer of modified reaction centers from Rhodobacter sphaeroides

能够还原球形红细菌修饰反应中心的细菌叶绿素二聚体的结合锰氧化物

• DOI: 10.1007/s11120-019--00680-3
• 发表时间: 2020
• 期刊: Photosynthesis research
• 影响因子: 3.7
• 作者: E. Espiritu, K. Chamberlain

Identification of amino acid residues in a proton release pathway near the bacteriochlorophyll dimer in reaction centers from Rhodobacter sphaeroides

• DOI: 10.1007/s11120-022-00968-x
• 发表时间: 2022-10-05
• 期刊: PHOTOSYNTHESIS RESEARCH
• 影响因子: 3.7
• 作者: Allen, J. P.;Chamberlain, K. D.;Williams, J. C.
• 通讯作者: Williams, J. C.