Biodynamic Imaging of Drug Resistance in Living Tissue

这项提议针对人类健康的两个重要领域，通过开发一种创新技术来提高癌症患者的化疗效果，以及用于感染性休克治疗患者的抗生素治疗。生物动力学成像是在之前的NSF拨款下开发的一种检测细胞内运动的方法，具有满足这一需求的潜力。该项目将扩展生物动态成像，以测量细胞信号对免疫疗法的反应，以及细菌感染的抗生素。生物动力学成像使用低相干数字全息术来检测来自体外和/或体外活体组织内不同细胞运动的细胞内多普勒信号。细胞运动是内源性的，并提供了一种功能成像方法来测量患者肿瘤或感染组织对治疗的药效反应。该项目的长期目标是在耐药性是有效治疗的主要障碍时，将生物动态成像作为首选的表型技术，用于选择个性化的医疗治疗。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This proposal targets two important areas of human health through the development of an innovative technology that will improve the efficacy of chemotherapy for cancer patients, and also for antibiotic treatments for patients with septic shock therapy. Biodynamic imaging, a method to detect intracellular motion developed under a previous NSF grant, has the potential to fill this need. This project will extend biodynamic imaging to measure cell signals in response to immunotherapies as well as antibiotics for bacterial infections. Biodynamic imaging uses low-coherence digital holography to detect intracellular Doppler signals from diverse cellular motions inside in vitro and/or ex vivo living tissue. Cellular motions are endogenous and provide a functional imaging approach to measure the pharmacodynamic response of patient tumors or infected tissues to treatment. The long-range goal of this project is to establish biodynamic imaging as the premier phenotyping technology for the selection of personalized medical treatment when drug resistance is the principal barrier to effective treatment.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.