Biodynamic Imaging of Drug Resistance in Living Tissue

活组织耐药性的生物动力学成像

• 批准号: 1911357
• 负责人: David Nolte
• 金额: $ 51.04万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2023-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1911357&HistoricalAwards=false
• 关键词: Biodynamic; Imaging; Drug; Resistance; Living

This proposal targets two important areas of human health through the development of an innovative technology that will improve the efficacy of chemotherapy for cancer patients, and also for antibiotic treatments for patients with septic shock therapy. Biodynamic imaging, a method to detect intracellular motion developed under a previous NSF grant, has the potential to fill this need. This project will extend biodynamic imaging to measure cell signals in response to immunotherapies as well as antibiotics for bacterial infections. Biodynamic imaging uses low-coherence digital holography to detect intracellular Doppler signals from diverse cellular motions inside in vitro and/or ex vivo living tissue. Cellular motions are endogenous and provide a functional imaging approach to measure the pharmacodynamic response of patient tumors or infected tissues to treatment. The long-range goal of this project is to establish biodynamic imaging as the premier phenotyping technology for the selection of personalized medical treatment when drug resistance is the principal barrier to effective treatment.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该提案通过开发一种创新技术，针对人类健康的两个重要领域，该技术将提高对癌症患者的化疗效果，以及对感染性休克治疗患者的抗生素治疗效果。生物动力成像，一种检测细胞内运动的方法，在以前的NSF资助下发展起来，有可能满足这一需求。该项目将扩展生物动力成像，以测量细胞信号对免疫疗法的反应，以及细菌感染的抗生素。生物动力学成像使用低相干数字全息技术来检测细胞内来自体外和/或离体活组织内不同细胞运动的多普勒信号。细胞运动是内源性的，提供了一种功能成像方法来测量患者肿瘤或感染组织对治疗的药效学反应。该项目的长期目标是在耐药性是有效治疗的主要障碍时，将生物动力成像作为选择个性化治疗的首要表型技术。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Biodynamic prediction of neoadjuvant chemotherapy response: Results from a prospective multicenter study of predictive accuracy among muscle-invasive bladder cancer patients

新辅助化疗反应的生物动力学预测：肌层浸润性膀胱癌患者预测准确性的前瞻性多中心研究结果

• DOI: 10.1016/j.urolonc.2022.11.017
• 发表时间: 2023
• 期刊: Urologic Oncology: Seminars and Original Investigations
• 作者: Laviana, Aaron A.;Schiftan, Elizabeth G.;Mashni, Joseph W.;Large, Michael C.;Kaimakliotis, Hristos Z.;Nolte, David D.;Turek, John J.;An, Ran;Morgan, Travis A.;Chang, Sam S.
• 通讯作者: Chang, Sam S.

Biodynamic optical assay for embryo viability

胚胎活力的生物动力学光学测定

• DOI: 10.1117/1.jbo.24.6.060502
• 发表时间: 2019
• 期刊: Journal of Biomedical Optics
• 影响因子: 3.5
• 作者: Li, Zhe;Ehmke, Natalie;Lorenzo, Ilka M.;Machaty, Zoltan;Nolte, David
• 通讯作者: Nolte, David

Phase-Sensitive Intracellular Doppler Fluctuation Spectroscopy

• DOI: 10.1103/physrevapplied.15.024043
• 发表时间: 2021-02-18
• 期刊: PHYSICAL REVIEW APPLIED
• 影响因子: 4.6
• 作者: Choi, Honggu;Jeong, Kwan;Nolte, David D.
• 通讯作者: Nolte, David D.

Doppler fluctuation spectroscopy of intracellular dynamics in living tissue

• DOI: 10.1364/josaa.36.000665
• 发表时间: 2019-04-01
• 期刊: JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
• 影响因子: 1.9
• 作者: Li, Zhe;Sun, Hao;Nolte, David D.
• 通讯作者: Nolte, David D.