Collaborative Research: A New Yeast Biomanufacturing Platform for Making High-value Products from Oils and Fats

合作研究：利用油脂生产高价值产品的新型酵母生物制造平台

• 批准号: 1911480
• 负责人: Dongming Xie
• 金额: $ 35.55万
• 依托单位: University of Massachusetts Lowell
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1911480&HistoricalAwards=false
• 关键词: Collaborative; Research; New; Yeast; Biomanufacturing

The United States produces more than 20 million tons of plant oils and animal fats every year. Used for food and feed applications, they generate low or limited economic value. The plant oils and fats wasted during food production negatively impact the environment. This project aims to develop a yeast to convert oils and fats into a series of high-value products. These products might include omega-3 fatty acids, carotenoids, and wax esters. If successful, this project could use yeast to create products that improve human health with minimal environmental impact. High school teachers will be trained in the lab, as will undergraduates and graduate students. Current outreach activities to the Texas School for the Deaf and a local K-5 elementary school will be augmented, as will efforts to recruit underrepresented minorities and women to pursue STEM training. Overall, the project will support the development of a workforce prepared for leadership in the growing bioeconomy industry.The oleaginous yeast Yarrowia lipolytica will be metabolically engineered to convert oils and fats into high-value products. Both cellular engineering and bioreaction engineering research will be integrated in this project to establish this biomanufacturing platform. First, the yeast will be engineered both metabolically and physiologically to facilitate the uptake of extracellular substrate and the bioconvesion of intracellular fatty acids. Second, the uptake of fatty acid substrates will be enhanced through modifying cellular transporters. Third, biosynthesis pathways will be created and optimized to convert the intracellular fatty acids into omega-3 fatty acids, wax esters, carotenoids, and other products. Forth, on the bioprocess side, mass transfer limitations of extracellular oils/fats/fatty acids in a bioreactor caused by the insolubility of the hydrophobic substrates in aqueous medium will be reduced through the use of a multi-phase (gas, oil, aqueous) computational fluid dynamics (CFD) model. Ultimately, a general bioreactor model will be built to describe processes for both extracellular mass transfer of oils/fats and the intracellular bioconversion of fatty acids into each specific high-value product.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

美国每年生产2000多万吨植物油和动物脂肪。用于食品和饲料应用，它们产生的经济价值很低或有限。食品生产过程中浪费的植物油脂对环境产生负面影响。该项目旨在开发一种酵母，将油脂转化为一系列高价值产品。这些产品可能包括ω-3脂肪酸，类胡萝卜素和蜡酯。如果成功，该项目可以使用酵母创造改善人类健康的产品，同时对环境的影响最小。高中教师将在实验室接受培训，本科生和研究生也将在实验室接受培训。 目前对得克萨斯州聋人学校和当地一所K-5小学的外联活动将得到加强，招募代表性不足的少数民族和妇女参加STEM培训的努力也将得到加强。总的来说，该项目将支持一支为在不断发展的生物经济行业中发挥领导作用做好准备的劳动力队伍的发展。将对产油酵母Yarrowia lipolytica进行代谢工程改造，将油脂转化为高价值产品。本计画将整合细胞工程与生物反应工程之研究，以建立此生物制造平台。首先，酵母将在代谢和生理上进行工程改造，以促进细胞外底物的摄取和细胞内脂肪酸的生物降解。第二，通过修饰细胞转运蛋白将增强脂肪酸底物的摄取。第三，将创建和优化生物合成途径，以将细胞内脂肪酸转化为ω-3脂肪酸、蜡酯、类胡萝卜素和其他产物。第四，在生物过程方面，通过使用多相（气体、油、水）计算流体动力学（CFD）模型，将减少由疏水底物在水性介质中的不溶性引起的生物反应器中细胞外油/脂肪/脂肪酸的传质限制。最终，将建立一个通用的生物反应器模型，以描述油/脂肪的细胞外质量转移和脂肪酸的细胞内生物转化为每种特定的高价值产品的过程。该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

Biomanufacturing of value‐added products from oils or fats: A case study on cellular and fermentation engineering of Yarrowia lipolytica

利用油脂生物制造增值产品：解脂耶氏酵母细胞和发酵工程案例研究

• DOI: 10.1002/bit.27685
• 发表时间: 2021
• 期刊: Biotechnology and Bioengineering
• 影响因子: 3.8
• 作者: Liu, Na;Soong, Ya‐Hue V.;Mirzaee, Iman;Olsen, Andrew;Yu, Peng;Wong, Hsi‐Wu;Xie, Dongming
• 通讯作者: Xie, Dongming

Microbial synthesis of wax esters

• DOI: 10.1016/j.ymben.2021.08.002
• 发表时间: 2021-08-24
• 期刊: METABOLIC ENGINEERING
• 影响因子: 8.4
• 作者: Soong, Ya-Hue Valerie;Zhao, Le;Xie, Dongming
• 通讯作者: Xie, Dongming