OPUS: CRS: Integrating organismal biology into the geographic mosaic of coevolution

OPUS：CRS：将有机生物学整合到共同进化的地理镶嵌中

• 批准号: 1911485
• 负责人: Edmund Brodie
• 金额: $ 36.28万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1911485&HistoricalAwards=false
• 关键词: OPUS; CRS; Integrating; organismal; biology

This project studies a predator-prey arms race between toxic newts and the garter snakes that eat them. The coevolutionary dynamics that push arms races such as this one onward are determined by many factors. The coevolutionary dynamics may involve the geographic distributions of the species, the physiological effects of toxins, or the structure of proteins that are used in attack or defense. Synthesizing how these different levels of biological organization interact and dictate arms races will help us better predict outcomes of coevolutionary battles. Understanding what drives one enemy to outpace the other, or eventually win the race, can inform strategies for managing interactions between humans and disease, hosts and parasites, and plants and their herbivore pests. This synthesis of several integrative studies will explore the connections between landscape ecology, microevolution processes, and underlying genetic and protein variation. Previous work on these newts and snakes has amassed thousands of physical specimens with associated phenotypic, genetic, and spatial data spread across multiple labs and collections. The proposed synthesis activities will aggregate these resources into a single relational database. These data will be used in spatially explicit analyses to test predictions about the historical, ecological, and environmental factors that structure geographic mosaics of coevolution. The intersection of organismal mechanisms and evolutionary process will be explored in a single-authored popular science book that follows the pathways to discoveries within the newt-snake system. The book will focus on how natural history observations led to hypotheses, and how tests of those ideas in turn generated new questions to pursue. Additional products include the publicly accessible database, three new papers from spatially explicit analyses, and a series of educational video trailers that introduce evolutionary concepts to high school audiences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个项目研究了有毒蝾螈和吃它们的吊袜带蛇之间的捕食者和猎物之间的军备竞赛。推动军备竞赛向前发展的共同进化动力是由许多因素决定的。共同进化的动力可能涉及物种的地理分布、毒素的生理效应或用于攻击或防御的蛋白质的结构。综合这些不同水平的生物组织如何相互作用并决定军备竞赛，将有助于我们更好地预测共同进化战斗的结果。了解是什么驱使一个敌人超过另一个敌人，或者最终赢得比赛，可以为管理人类与疾病、宿主与寄生虫、植物与它们的食草动物害虫之间的互动提供策略。这几项综合研究将探索景观生态学、微进化过程以及潜在的遗传和蛋白质变异之间的联系。之前对这些蝾螈和蛇的研究已经积累了数千个物理标本，以及分布在多个实验室和收藏中的相关表型、遗传和空间数据。拟议的综合活动将把这些资源聚集到一个单一的关系数据库中。这些数据将被用于空间显式分析，以测试对构成共同进化的地理马赛克的历史、生态和环境因素的预测。有机体机制和进化过程的交集将在一本单一作者的科普书籍中进行探索，这本书遵循了在纽特-蛇系统内发现的途径。这本书将专注于自然历史观察如何导致假说，以及对这些想法的测试如何产生新的问题来追寻。其他产品包括可公开访问的数据库，来自空间显性分析的三篇新论文，以及一系列向高中观众介绍进化论概念的教育视频预告片。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Sex linkage of the skeletal muscle sodium channel gene (SCN4A) explains apparent deviations from Hardy–Weinberg equilibrium of tetrodotoxin-resistance alleles in garter snakes (Thamnophis sirtalis)

• DOI: 10.1038/s41437-020-0300-5
• 发表时间: 2020-02
• 期刊: Heredity
• 影响因子: 3.8
• 作者: Kerry L. Gendreau;Michael T. J. Hague;C. Feldman;Edmund D. Brodie;Edmund D. Brodie;J. McGlothlin

The geographic mosaic in parallel: Matching patterns of newt tetrodotoxin levels and snake resistance in multiple predator–prey pairs

平行的地理马赛克：多个捕食者与猎物对中蝾螈河豚毒素水平和蛇抗性的匹配模式

• DOI: 10.1111/1365-2656.13212
• 发表时间: 2020
• 期刊: Journal of Animal Ecology
• 影响因子: 4.8
• 作者: Reimche, Jessica S.;Brodie, Jr., Edmund D.;Stokes, Amber N.;Ely, Erica J.;Moniz, Haley A.;Thill, Vicki L.;Hallas, Joshua M.;Pfrender, Michael E.;Brodie, III, Edmund D.;Feldman, Chris R.
• 通讯作者: Feldman, Chris R.

Tetrodotoxin levels in lab-reared Rough-Skinned Newts (Taricha granulosa) after 3 years and comparison to wild-caught juveniles

实验室饲养的粗皮蝾螈（Taricha capsulosa）3年后的河豚毒素水平与野生捕获的幼鱼的比较

• DOI: 10.1016/j.toxicon.2022.04.007
• 发表时间: 2022
• 期刊: Toxicon
• 影响因子: 2.8
• 作者: Gall, Brian G.;Stokes, Amber N.;Brodie, Edmund D.;Brodie, Edmund D.
• 通讯作者: Brodie, Edmund D.

The Epigenome and Beyond: How Does Non-genetic Inheritance Change Our View of Evolution?

• DOI: 10.1093/icb/icab084
• 发表时间: 2022-02-05
• 期刊: INTEGRATIVE AND COMPARATIVE BIOLOGY
• 影响因子: 2.6
• 作者: Brodie, Edmund D., III;Gregory, Brian;Riddle, Nicole C.
• 通讯作者: Riddle, Nicole C.

The road not taken: Evolution of tetrodotoxin resistance in the Sierra garter snake (Thamnophis couchii) by a path less travelled.

未走的路：塞拉袜带蛇（Thamnophis couchii）的河豚毒素抗性沿着一条少有人走的路进化。

• DOI: 10.1111/mec.16538
• 发表时间: 2022
• 期刊: Molecular ecology
• 影响因子: 4.9
• 作者: Reimche,JessicaS;DelCarlo,RobertE;BrodieJr,EdmundD;McGlothlin,JoelW;Schlauch,Karen;Pfrender,MichaelE;Brodie3rd,EdmundD;Leblanc,Normand;Feldman,ChrisR
• 通讯作者: Feldman,ChrisR